LOCUS       sequence-20389-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG413GAL-ccdB
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(129..253)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(286..302)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(287..303)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      305..552
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(321..339)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(570..589)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(571..589)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(599..616)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(599..615)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(608..630)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tctccgttat cggggaagag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
       61 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
      121 aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
      181 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
      241 tgtacaaagt ggtgatgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct
      301 cgagtcatgt aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta
      361 accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt
      421 atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc
      481 gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag
      541 gctttaattt gcggccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
      601 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      661 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
      721 cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag
      781 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca c
//
