LOCUS       sequence-203898-        9196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression retroviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Lindsey JC, Kawauchi D, Schwalbe EC, Solecki DJ, Selby MP, McKinnon 
            PJ, Olson JM, Hayden JT, Grundy RG, Ellison DW, Williamson D, Bailey
            S, Roussel MF, Clifford SC
  TITLE     Cross-species epigenetics identifies a critical role for VAV1 in SHH
            subgroup medulloblastoma maintenance.
  JOURNAL   Oncogene. 2015 Sep 3;34(36):4746-57. doi: 10.1038/onc.2014.405. Epub
            2014 Dec 22.
  PUBMED    25531316
REFERENCE   2  (bases 1 to 9196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/onc.2014.405"; journalName: "Oncogene"; date: "2015-09-3- 
            3"; volume: "34"; issue: "36"; pages: "4746-57"
COMMENT     Sequence Label: MSCV mouse Vav1 IRES GFP
FEATURES             Location/Qualifiers
     source          1..9196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1512..1521
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4151..4699
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4295..4312)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4522..4541
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4708..4710,4711..4713,4714..5427)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4708..4710,4711..4713,4714..5424)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4753..4774)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5014..5033)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5361..5382
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5589..6103
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6265..6281
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6289..6295,6296..6313,6314..6319))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6334..6355
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6472..6489)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6643..7231)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6723..6742)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7402..8193,8194..8262))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8025..8044
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8263..8367)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8435..8453
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8491..8513)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8613..8632
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8826..8848
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8976..8995
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9195..9196,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaattg ggatcccagt gtggtggtac gtaggaattc
     1441 agatctggcg gcggtggtga aggaacgagg gtgcacgggg cctccccgag gggccaagtg
     1501 agaggccggc agccaccatg gagctctggc gacagtgcac ccactggctg atccagtgtc
     1561 gggtgctgcc tcccagccac cgtgtgacct gggagggggc ccaggtgtgt gagctggcac
     1621 aggcactgcg ggacggtgtc ctcttgtgcc aattgcttaa caacctgctt ccccaggcca
     1681 ttaatcttcg cgaggttaac ttgcggcccc agatgtccca gttcctttgt cttaagaaca
     1741 ttcgaacctt cctgtctact tgctgtgaga agttcggcct caagcgcagt gaactctttg
     1801 aggcttttga cctcttcgat gtgcaggact ttggaaaggt catctacacc ctgtctgctc
     1861 tgtcatggac acccattgcc cagaacaaag gaatcatgcc cttcccaaca gaggacagcg
     1921 ctctgaacga cgaagatatt tacagtggcc tttcagacca gattgatgac accgcagagg
     1981 aagacgagga cctttatgac tgcgtggaaa atgaggaggc agagggggac gagatctacg
     2041 aggacctaat gcgcttggag tcggtgccta cgccacccaa gatgacagag tatgataagc
     2101 gctgctgctg cctgcgggag atccagcaga cggaggagaa gtatacagac acactgggct
     2161 ccatccagca gcacttcatg aagcctctgc agcgattcct taagcctcaa gacatggaga
     2221 ccatctttgt caacattgag gagctgttct ctgtgcatac ccacttctta aaggaactga
     2281 aggatgccct ggctggcccg ggagcaacaa cactgtatca ggtcttcatc aagtacaagg
     2341 agaggttcct ggtttatggc cgttattgca gtcaggtgga gtcagccagc aagcacttgg
     2401 atcaagtggc cacagcacgg gaggatgtgc agatgaagct ggaggaatgt tctcaaagag
     2461 ctaacaatgg ccgattcacc ctacgggatc tgctgatggt acctatgcag cgggtgctga
     2521 agtaccacct ccttctccag gagctagtga aacacacaca ggatgctaca gagaaggaga
     2581 acctgcggtt ggccctggac gccatgaggg acctggcaca gtgcgtgaac gaggtcaaga
     2641 gggacaatga aaccctacgg cagatcacaa actttcagct gtccattgag aacctggacc
     2701 agtctctggc taactatggc cggcccaaga ttgacggtga gctcaagatt acctcagtgg
     2761 agcgtcgctc aaagacagac aggtatgcct tcctgctgga caaagcactg ctcatctgta
     2821 aacgccgcgg ggactcttac gacctcaaag cctcggtgaa cttgcacagc ttccaagttc
     2881 gagatgactc ctccggggag cgagacaaca agaagtggag ccatatgttc cttctgattg
     2941 aggatcaagg cgcccagggc tatgagctgt tcttcaagac tcgggagctg aagaagaagt
     3001 ggatggaaca gttcgaaatg gccatctcca acatttaccc agagaatgct acagccaatg
     3061 ggcatgattt tcagatgttc tcctttgagg agaccacttc ctgcaaggcc tgccagatgt
     3121 tactcagagg cacattctac cagggatatc gctgttacag gtgccgggca cctgcacaca
     3181 aggagtgtct ggggagagtg cctccctgtg gtcgccatgg gcaagatttc gcaggaacca
     3241 tgaagaagga caagctccat cgaagggccc aggacaagaa aaggaatgaa ttgggtctgc
     3301 ctaagatgga agtgtttcag gaatactatg ggatccctcc tccacctgga gcctttgggc
     3361 catttttacg gctcaaccct ggggacattg tggagctcac taaggcagag gctgagcaca
     3421 actggtggga gggaaggaat actgctacaa atgaagtcgg ctggtttccc tgtaacagag
     3481 tgcatcccta tgtccacggc cctcctcagg acctgtctgt gcatctctgg tatgcgggcc
     3541 ctatggaacg agcaggcgct gagggcatcc tcaccaaccg ttctgatggg acctatctgg
     3601 tgcggcagag ggtgaaagat acagcggagt tcgccatcag cattaagtat aacgtggagg
     3661 tcaagcatat taaaatcatg acgtcagagg ggttgtaccg gatcacagag aagaaggctt
     3721 tccggggcct tctggaactg gtagagtttt atcagcagaa ttccctcaaa gattgcttca
     3781 agtcgttgga caccaccttg cagtttcctt ataaggaacc tgagaggaga gccatcagca
     3841 agccaccagc tggaagcacc aagtattttg gcactgccaa agcccgctac gacttctgtg
     3901 cccgggacag gtcggaactg tcccttaagg agggtgatat catcaagatc ctcaataaga
     3961 agggacagca aggctggtgg cgtggggaga tctacggccg gatcggctgg ttcccttcta
     4021 actatgtgga ggaagactat tccgaatatt gctgacctgg tgccctgtag gacacagaga
     4081 gaggcagatg aaggctgagc ccaggatgct agagtatccc tcgagcggga tcaattccgc
     4141 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     4201 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     4261 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     4321 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     4381 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     4441 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     4501 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     4561 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     4621 tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     4681 tttgaaaaac acgataatac ctgtacaatg gtgagcaagg gcgaggagct gttcaccggg
     4741 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     4801 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     4861 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     4921 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     4981 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     5041 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     5101 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     5161 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     5221 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     5281 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     5341 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     5401 ctcggcatgg acgagctgta caagtaaagc ggccgcgact ctagagtcga cctgcaggca
     5461 tgcaagcttc aggtagccgg ctaacgttaa caaccggtac ctctagaact atagctagca
     5521 tgcgcaaatt taaagcgctg atatcgataa aataaaagat tttatttagt ctccagaaaa
     5581 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
     5641 gcaaggcatg gaaaatacat aactgagaat agagaagttc agatcaaggt taggaacaga
     5701 gagacagcag aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     5761 gggccaagaa cagatggtcc ccagatgcgg tcccgccctc agcagtttct agagaaccat
     5821 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
     5881 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
     5941 ccacaacccc tcactcggcg cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg
     6001 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
     6061 tctcctctga gtgattgact acccgtcagc gggggtcttt catgggtaac agtttcttga
     6121 agttggagaa caacattctg agggtaggag tcgaatatta agtaatcctg actcaattag
     6181 ccactgtttt gaatccacat actccaatac tcctgaaata gttcattatg gacagcgcag
     6241 aaagagctgg ggagaattgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6301 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6361 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     6421 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6481 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6541 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6601 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6661 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6721 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6781 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6841 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6901 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6961 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7021 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7081 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7141 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7201 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7261 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7321 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7381 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7441 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7501 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7561 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7621 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7681 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     7741 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     7801 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     7861 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     7921 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     7981 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     8041 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8101 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8161 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8221 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8281 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8341 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     8401 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     8461 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     8521 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     8581 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     8641 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     8701 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     8761 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     8821 gttttcccag tcacgacgtt gtaaaacgac ggcgcaagga atggtgcatg caaggagatg
     8881 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc
     8941 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca
     9001 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggcgatta
     9061 gtccaatttg ttaaagacag gatatcagtg gtccaggctc tagttttgac tcaacaatat
     9121 caccagctga agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc
     9181 cagaaaaagg ggggaa
//
