LOCUS       sequence-203905-        7284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  heat shock inducible transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Strzyz PJ, Lee HO, Sidhaye J, Weber IP, Leung LC, Norden C
  TITLE     Interkinetic nuclear migration is centrosome independent and ensures
            apical cell division to maintain tissue integrity.
  JOURNAL   Dev Cell. 2015 Jan 26;32(2):203-19. doi: 
            10.1016/j.devcel.2014.12.001. Epub 2015 Jan 15.
  PUBMED    25600237
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.12.001"; journalName: "Dev Cell"; date: 
            "2015-01-26- 26"; volume: "32"; issue: "2"; pages: "203-19"
COMMENT     Sequence Label: HS-DN-Plk1
FEATURES             Location/Qualifiers
     source          1..7284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1587..1616
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3726..3742)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3726..3742)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    3766..3900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3790..3809)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3844..3863
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4013..4033)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4013..4031)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4052..4068)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4052..4068)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4065..4087)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4076..4092
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4100..4106,4107..4124,4125..4130))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4145..4166
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4283..4300)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4454..5042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4534..4553)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5213..6004,6005..6073))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5836..5855
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6074..6178)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6204..6659)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6341..6362)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6553..6572
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcaggggtgt cgcttggtta tttccaaaaa tcaaattaat tttattaaac tattagaacg
       61 agcatgtttt gtctatatgc tacagaagat aaaaaataat aggagttaac agttataaaa
      121 caacacactt tgtttctatt gattgttgac cacactgggg tctcattaag ttagattaaa
      181 gacacactaa ctgggtcaaa agcagcagat tgatttcata gcaccagggt aaactttcta
      241 acacttttac ggcaatcata tacattaaaa ttaaatacag accacgactg aacaaggagg
      301 atgatctcca atattaaaca aagagacttg tgcctatttc tctgagggta aacatgacct
      361 ctcaagttag caagttgttt ttaacactac aaaaatagtt aagactgcaa tcccagaata
      421 aagtattggt tttaaccaat caatatagta cagtaaacat ccatttgttt tgttgaaacg
      481 ttaaacaaat ctgaccaaag ctattagctt atataaaaca ggtttgcctt ctatgtagct
      541 gaaaacacca caggcccgat tttgctactg tgtaaaacat ttcagcaaga tttttttatt
      601 gcattttttt ttactgaatc gttcaaacat tttatcattt tagtttgttc attcattgca
      661 actggaaaaa caacacatca cacaaccgca catatttcag caataagtac aataaaacac
      721 tcaaataaaa aaaacatttt aaatctcttt gtatttttga ccgctgtttc gcgtaatttc
      781 acggtaaaac tctggaaatc tccactacat tcctctcagc ggctcctctc aatgacagct
      841 gaagaagtga cgcggctgcc tgctgtgttt tgattggtcg aattcactgg aggcttccag
      901 aacagtgtag agtctgaacg ggtgcgcgct ctgctgtatt taaagggcga aagagagacc
      961 gcagagaaac tcaaccgaag agaagcgact tgacaaagaa gaaaagagca gcctgacagg
     1021 actttttccc cgacgaggtg tttattcgct ctatttaaga atctactgta aggtaagtct
     1081 caatatattg tactctattg gctaatcaga attatataga gattatatgt acttaatgtc
     1141 aaaaaatcaa ctttgtatat gtaatctttt tacatgtgga ctgcctatgt tcatcttatt
     1201 ttaggtctac tagaaaatta tatttcccgt tttcacaata aggattttta aaaaaagcaa
     1261 tgaacagacg ggcatttact ttatgttgct gacattattt tatatgagca taataaccat
     1321 aaatactagc aaatgtccta aatgaatttg tgttaatgtt gtctacaaaa gaaaattagc
     1381 gttttacttg tacaactaat aataacttgg ttattaagag aatttcactt gttgactaga
     1441 aaaatccttt cataatgaaa caattgcacc ataaattgta taaatataaa attaattcta
     1501 attgtttttt tttcctgcag tcgaagcttg cttacatttg cttctgacac aactgtgttc
     1561 actagcaacc tcaaacagac accatggagc agaagctgat ctccgaggag gacctgaaca
     1621 tgaattcctg cagcccggga tccatgagtg ctgcagtgac tgcagggaag ctggcacggg
     1681 caccggccga ccctgggaaa gccggggtcc ccggagttgc agctcccgga gctccggcgg
     1741 cggctccacc ggcgaaagag atcccggagg tcctagtgga cccacgcagc cggcggcgct
     1801 atgtgcgggg ccgctttttg ggcaagggcg gctttgccaa gtgcttcgag atctcggacg
     1861 cggacaccaa ggaggtgttc gcgggcagga ttgtgcctaa gtctctgctg ctcaagccgc
     1921 accagaggga gaagatgtcc atggaaatat ccattcaccg cagcctcgcc caccagcacg
     1981 tcgtaggatt ccacggcttt ttcgaggaca acgacttcgt gttcgtggtg ttggagctct
     2041 gccgccggag gtctctcctg gagctgcaca agaggaggaa agccctgact gagcctgagg
     2101 cccgatacta cctacggcaa attgtgcttg gctgccagta cctgcaccga aaccgagtta
     2161 ttcatcgaga cctcaagctg ggcaaccttt tcctgaatga agatctggag gtgaaaatag
     2221 gggattttgg actggcaacc aaagtcgaat atgacgggga gaggaagaag accctgtgtg
     2281 ggactcctaa ttacatagct cccgaggtgc tgagcaagaa agggcacagt ttcgaggtgg
     2341 atgtgtggtc cattgggtgt atcatgtata ccttgttagt gggcaaacca ccttttgaga
     2401 cttcttgcct aaaagagacc tacctccgga tcaagaagaa tgaatacagt attcccaagc
     2461 acatcaaccc cgtggccgcc tccctcatcc agaagatgct tcagacagat cccactgccc
     2521 gcccaaccat taacgagctg cttaatgacg agttctttac ttctggctat atccctgccc
     2581 gtctccccat cacctgcctg accattccac caaggttttc gattgctccc agcagcctgg
     2641 accccagcaa ccggaagccc ctcacagtcc tcaataaagg cttggagaac cccctgcctg
     2701 agcgtccccg ggaaaaagaa gaaccagtgg ttcgagagac aggtgaggtg gtcgactgcc
     2761 acctcagtga catgctgcag cagctgcaca gtgtcaatgc ctccaagccc tcggagcgtg
     2821 ggctggtcag gcaagaggag gctgaggatc ctgcctgcat ccccatcttc tgggtcagca
     2881 agtgggtgga ctattcggac aagtacggcc ttgggtatca gctctgtgat aacagcgtgg
     2941 gggtgctctt caatgactca acacgcctca tcctctacaa tgatggtgac agcctgcagt
     3001 acatagagcg tgacggcact gagtcctacc tcaccgtgag ttcccatccc aactccttga
     3061 tgaagaagat caccctcctt aaatatttcc gcaattacat gagcgagcac ttgctgaagg
     3121 caggtgccaa catcacgccg cgcgaaggtg atgagctcgc ccggctgccc tacctacgga
     3181 cctggttccg cacccgcagc gccatcatcc tgcacctcag caacggcagc gtgcagatca
     3241 acttcttcca ggatcacacc aagctcatct tgtgcccact gatggcagcc gtgacctaca
     3301 tcgacgagaa gcgggacttc cgcacatacc gcctgagtct cctggaggag tacggctgct
     3361 gcaaggagct ggccagccgg ctccgctacg cccgcactat ggtggacaag ctgctgagct
     3421 cacgctcggc cagcaaccgt ctcaaggcct cctaatagct gccctcccct ccggactggt
     3481 gccctcctca ctcccacctg catctggggc ccatactggt tggctcccgc ggtgccatgt
     3541 ctgcagtgtg ccccccagcc ccggtggctg ggcagagctg catcatcctt gcaggtgggg
     3601 gttgctgtat aagttatttt tgtacatgtt cgggtgtggg ttctacagcc ttgtccccct
     3661 ccccctcaac cccaccatat gaattgtaca gaatatttct attgaattcg agctcggtac
     3721 ccggggatcc actagttcta gaactatagt gagtcgtatt acgtagatcc agacatgata
     3781 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     3841 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     3901 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     3961 taattcgcgg ccgcggcgcc aatgcattgg gcccggtacc cagcttttgt tccctttagt
     4021 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     4081 atccgctcac aattccacac aacatacgag ccgggagcat aaagtgtaaa gcctggggtg
     4141 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     4201 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     4261 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4321 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4381 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4441 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4501 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4561 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4621 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4681 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4741 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4801 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4861 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4921 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4981 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5041 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5101 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     5161 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     5221 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5281 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5341 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5401 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5461 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5521 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5581 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5641 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5701 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5761 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5821 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5881 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5941 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     6001 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     6061 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     6121 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     6181 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     6241 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     6301 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     6361 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     6421 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     6481 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     6541 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     6601 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     6661 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     6721 attacgccag tcgaccgcca attcaatatg gcgtatatgg actcatgcca attcaatatg
     6781 gtggatctgg acctgtgcca attcaatatg gcgtatatgg actcgtgcca attcaatatg
     6841 gtggatctgg accccagcca attcaatatg gcggacttgg caccatgcca attcaatatg
     6901 gcggacctgg cactgtgcca actggggagg ggtctacttg gcacggtgcc aagtttgagg
     6961 aggggtcttg gccctgtgcc aagtccgcca tattgaattg gcatggtgcc aataatggcg
     7021 gccatattgg ctatatgcca ggatcaatat ataggcaata tccaatatgg ccctatgcca
     7081 atatggctat tggccaggtt caatactatg tattggccct atgccatata gtattccata
     7141 tatgggtttt cctattgacg tagatagccc ctcccaatgg gcggtcccat ataccatata
     7201 tggggcttcc taataccgcc catagccact cccccattga cgtcaatggt ctctatatat
     7261 ggtctttcct attgacgtca tatg
//
