LOCUS       sequence-203910-       13362 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  transgenesis, ubiquitous expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13362)
  AUTHORS   Daniel K, Icha J, Horenburg C, Muller D, Norden C, Mansfeld J
  TITLE     Conditional control of fluorescent protein degradation by an 
            auxin-dependent nanobody.
  JOURNAL   Nat Commun. 2018 Aug 17;9(1):3297. doi: 10.1038/s41467-018-05855-5.
  PUBMED    30120238
REFERENCE   2  (bases 1 to 13362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-08-17"; volume: "9(1):3297. doi"; pages: " 
            10.1038/s41467-018-05855-5"
COMMENT     Sequence Label: bactin:GFP-PCNA
FEATURES             Location/Qualifiers
     source          1..13362
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(1075..1086,1087..1249)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1622..1638
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1658..1678
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6985..7009
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7012..7032
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      7045..7054
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7051..7053,7054..7056,7057..7767)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7096..7117)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7357..7376)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7704..7725
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(8609..8633)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8635..8769
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(8832..8852)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(8860..8876)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    8892..9026
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(9465..9652,9653..9664)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9698..9714)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9698..9714)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9751..9771)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9751..9769)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9790..9806)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9803..9825)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9814..9830
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9838..9844,9845..9862,9863..9868))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9883..9904
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10021..10038)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10192..10780)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10272..10291)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10951..11742,11743..11811))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11574..11593
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11812..11916)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11942..12397)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12079..12100)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12291..12310
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12524..12546
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12539..12555
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12565..12584
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12565..12583
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
       61 gattgattga ttgattgatt ggtagtcaaa ataagaaata atttccacag attcattaca
      121 gaaatgatta aatgcataca taaaaaactg gggggggggg ggatacaaca acacacttaa
      181 gtcacatttg cctacgtaaa gaaaagtaaa gaaaatcaat agctatattt tacatctcct
      241 ttttttgctg tcttttaatt agccttgttt tgctgttatc ttgattgaaa ctgtaacttc
      301 ttcacctgct tcttttcttt gtaggttttg ccagcccaga aacaggcatg gctgtgcaag
      361 gccagagcac catgcacaat gcgctgcatg tcttcatgaa cggctcaatg tcctcagtcc
      421 agggctcagc caacgacccc atcttcctcc ttcaccatgc tttcattgac aggtaacaaa
      481 cacgtcatga cattagactg cacagttttt gacaaagttc atacaatctg ttgtttatag
      541 ctgctacaat tagtgaagtt tgtgaatgta cttggatgag cagcgaaaga tcaattgaga
      601 tcaattgtta gagtttggtt gccctgcaga gcaaagaaca aaaaataatc tggtggcttt
      661 actgcgtgag gttattattg gtggaataga aacacaaaac ataattgcat ttatttgttt
      721 aattttttat cttatcttaa ctttcatctt gcatatttgt ttcttacatc atttctagca
      781 tctttgagcg ctggctaaga actcatcagc ctccccggtc catctaccca cgtaccaatg
      841 caccaattgg ccacaatgac ggctactaca tggtgccatt ccttcctctt tataggaatg
      901 gagactacct cctgtccaac aatgctcttg gatacgagta cgcctacctg ttggacccag
      961 gtcattgcac aacaccagaa atgccctctg atctgcaaaa gacgtgaata tctgttcaga
     1021 cacccatatc cactctgttc cacacaggtc agaggtttgt ccaggagttc ttgacagagg
     1081 tgtaaaaagt actcaaaaat tttactcaag tgaaagtaca agtacttagg gaaaatttta
     1141 ctcaattaaa agtaaaagta tctggctaga atcttacttg agtaaaagta aaaaagtact
     1201 ccattaaaat tgtacttgag tattaaggaa gtaaaagtaa aagcaagaaa gaaaactaga
     1261 gattcttgtt taagctttta atctcaaaaa acattaaatg aaatgcatac aaggttttat
     1321 cctgctttag aactgtttgt atttaattat caaactataa gacagacaat ctaatgccag
     1381 tacacgctac tcaaagttgt aaaacctcag atttaacttc agtagaagct gattctcaaa
     1441 attgttagtg tcaagcctag ctcttttggg gctgaaaagc aatcctgcag tgctgaaaag
     1501 cctctcacag gcagccgatg cgggaagagg tgtattagtc ttgatagaga ggctgcaaat
     1561 agcaggaaac gtgagcagag actccctggt gtctgaaaca caggccagat gggccctcga
     1621 gcaggaaaca gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttgaa
     1681 aattccagtt tgaagaaact tttcaagact caccttttca ctgttggtct taatttaggt
     1741 tgattatttt tgtcaattta ataattacat tgtattgttc atattttatt gtcttattgt
     1801 ggtgttttta cagctttttt attgttctgc tttgttactg tttgccttgt atgcattgta
     1861 gcgctttggg tctgagaaaa gcgcactata aataaactgt attattatta ttagggatgg
     1921 acatcacggt ggcgcgatgg gtggcacaat tacctcacag caagtaggtt gctgtttaga
     1981 gccccggctg gatcagttgg cagttctgtg tggagtttgc atgttctccc tgttcaagta
     2041 ggtttcctcc cggtgctctg gtttcccctc acaagtccaa agacatgatg tataggtgaa
     2101 tggggtaagc taaattgacc atagtgagtg tgtgtgtgtg tgtgaatgag agtgtatagg
     2161 tgtttcccag tgttaggttg caactggaag gacatctgct gtgtaaaaca tatgcaggat
     2221 aagttggcgg ttcattcccc tatggtgacc cctggttaat taaaggacta agttgaaaat
     2281 gaatgaatga atgaatgatt agagataaac cgataccact tttttacaaa ccgatatgag
     2341 tacaagtata atagtttctg ttactcgaca atactgagta ccgattctga tacttcttgg
     2401 atttggtttc aatgtaatta atttgaaaga aggttttatt tcttgcctgt cgcaaggatg
     2461 aacacaaaaa tctttaccag aaggatattt taagctaaga atatacgcac gttgttgaca
     2521 accctgtatc gattaccgat taaacgtgga catttaaagg ataagttatg cttgcgccat
     2581 cacaacttta cacattacat gatcaaagat catattgaat attcggttta ttcgtcagaa
     2641 gcctatagat ttgttaaaag tgtattttat aaacggatgg tttataaaga ctaagctatt
     2701 aatatatcag acattcatta atacatcaag acatcgatgt caggtaggac aacaaatcac
     2761 atccatgtaa tgaaatcttg ttgagtggta ttggtgcgtg gtttcagcat ttcatgtgca
     2821 atacgagtaa gaatatttta accaagtatc gacccaaaac ctgatactgg tatcggtgca
     2881 tcccttatta ttattattat caaaaacaca cttgttttat ttatgtgggt gccaaacaac
     2941 aaactatgaa atgcagcatg acacgcccaa aaagtgttcc tttgagaaca acttcataaa
     3001 tatgtttctt atcattatgc atgaatggtg tactgggaga gctttggtat cagaattgag
     3061 ataagagact ttggcattct aaaggacaat gacattatca cactgacagt caacaacact
     3121 ttattgctct ggaaatctta tgtcatgggg caaagctgag gttcattagg acagtacctt
     3181 atttagagat gaatgggaag gctgtagtgg gctgctatca gggaagggtg gtggtcctcg
     3241 ggggaactat ggactcacaa aagcacatct gctgtaacaa gatcccctgt gcaatctaca
     3301 aaagttacaa agagaagcat ggttggttaa aaaatagaat tgactggata tgctgaatgc
     3361 attgtttgtt gtgtgttaca tcacattaac cacagattgg tgctaaatat ttgcttatct
     3421 tataaaaacc tgtgtgttta taaagggggg tgtggatgga tgcttggcat tctttgaaaa
     3481 gatgttgggg gatggaagga ggagacctta ttgatgagta tccagtttgc agataacaag
     3541 tttttagaca ttcatactat gatgctgttt atgtctgttt tttctcattt tttctataca
     3601 caaacagcta taaaacattc acttttatat ctgatgctgt atattaaatt tcgttgtatt
     3661 tattctgttt gctctgttgg gtcatagcta gggccagaca gaatctgctg acattttttg
     3721 ctttttctgc agagaatttt gtaaacaatc tgtgatttat gcgagatttt ttcgggagta
     3781 tcgtaactaa aaacttacta tatgaaataa aaaaaaataa taccttttta acttttaata
     3841 aatatataca atgcaagtcc acctagatcc aattatttgg tcgacaaagc aagtctctca
     3901 cataatatat cttctaaaag acagaaaaca ttactttcag accatattgt aatcaaatga
     3961 acactttcat attagtcaat aatattacgg aaatgtattt aaaaactgaa taaacatata
     4021 tttacacctt tgtctttatt tctagagtgc ttttacaatg tagattgtgt caaagcagct
     4081 taacttagag gattagtata gtaaattgaa actgtgtcag tccatttttt aagagtttaa
     4141 gttcagttca gtttagttca gtgtgagtta atttttactg ctgagagtcc aaacactaaa
     4201 gagcaaatcc aaatacacaa gtcaataaat aaactcaatg atgggctaaa gatctgggga
     4261 aatctgcaga tttctgtgcg cacagattct gtgtgggcct agtcattacc tatactcaac
     4321 gtttactctg agaaacttcc atattttaga ggtaaaatgc tcagaattgt aaaaagtctt
     4381 gtcaaaatac ttggaatttg ccaaaaacaa aataaaagtc acatcacctc attgtccttg
     4441 gtatcagcaa gcacttttaa cacaatcagc tgtttttgtt tataactccc actgaaggcc
     4501 atgtgcatta ctcccaagtt acacagtaaa aagtcacatg tagtttatca tgatcactgc
     4561 tgctcaagca cagctttatc tgttattcag tgtggcaata tctgaaatgt gtgcaaaata
     4621 ttatttattg tagctgatca ccaacacttt cagtgttttt tttttctact tttaatgaga
     4681 attatgttct taggatttat ccttataaac ctctttaaaa agaaatgtta tgtctgcata
     4741 gacacgacat gaaattatga acagaaaatt taactattta agatcaagac ttgacatata
     4801 acatttctaa tgaacattaa acctgaggta ggcccacttg caaaaatgta actgctatca
     4861 ctcactttat acatcattgt gtaacttgta cacacaaaaa tagtgaagca atgtggcatg
     4921 gaatgcaggt cccattcatt caaagttatt aaagcacttt cttaaagtgc attactactg
     4981 agctaagacc agctagttgc tcatacaaca tactacaagt agtgtcatcc taatttggat
     5041 aaaaaaaatg tccattttat acatggaaaa gtactcatta gatcccccat tgtttgtatt
     5101 acggtatttc gtgaacacaa gaggtaaatg acctacagag ctgctgttgt gttagattga
     5161 aaacacaaca caggatcatg gaggcatcca tcattatgac gcattctgcc acttgtaact
     5221 ctgcacagac tttttgaaaa gtttaattga gtcttggcgt tgtcgcagaa aaatacgaaa
     5281 aattctgtgt taaaatttgc aaataaaaat tccagtttta taggaaatcg ccatggcctt
     5341 gttctttaac cagttaagct tccccttctt tcactctcaa gttgcaagaa gcaagtgtag
     5401 caatgtgcac gcgacagccg ggtgtgtgac gctggaccaa tcagagcaca gagctccgaa
     5461 agtttacctt ttatggctag agccgggtat gtgccgtcat ataaaagagc gcgcccagct
     5521 tttcagcctc actttgagct cctccacacg cagctagtgc ggaatatcat ctgcttgtaa
     5581 cccattctct taagtcgaca accccccaaa cccaaggtga gttggtttta cagtttttaa
     5641 gcgtttaatt atagtttcta tctattaatg ttaagtaaaa tgtgtaaatg gataaatatg
     5701 atttgatgaa gtctttagac agttcataac ggtctctttg tagttatcta ctaggtttga
     5761 tttataagtg gtggttcttt tgataatcta tgttacattt aataaatatt ggctgtatct
     5821 taactttttt gtagcgttag tggatgtggc aggtgagaat gtcggtaatt taacgtgacc
     5881 agtcgtaggc acgacattga acattgaata ggccggtgtg aaatgggtgt taagtcttaa
     5941 tataaagttg tgttctggct aatctctctt taacagccat aaatgtgatt tttaactaca
     6001 gttttgaggg tgctggtgag catgttgcac acttgatgga tagccggcat gggaagttct
     6061 ttgtgcaggc agtgctgcag cagggtgtga cctactttag ctagccggct aaccagctct
     6121 catctgatgt aacgtaaacc ccatcaatct aacatcggct cattcgtgct ttataagatc
     6181 gaagatcttg ttattagtaa cgggtattgg catttagttt taacttggtt aattttcttt
     6241 ttgttatgtg ttctatttaa atgtagcctg cagccccctc tgtctttaat gaagctgaag
     6301 attaacgtta acgttagtga agtgttgtat ccgctatgaa agatttgaat tttagaggtg
     6361 tggtccactt taataacggc tgcggagttc tctggctgca cttcagccgc tgtgactgtc
     6421 gaagcagagc ccatcagttt ttcaggatct cggtgatttt tgaggctaga ctatgaactg
     6481 aaccgactga gagtgaccgt ccagaaatgc tttagtgtga agtctccgct cacgaggagt
     6541 ccgtggactg actgcagatc tgtgacgcag taacacccgg cggcagacac ccgttgaaat
     6601 gcggttgtgt aattgatcgc aggcgagggt ggaagaggat gtgaaacttc atttgtgtaa
     6661 aatttaggga gtggtcctgg tgtgatgaat gtcgaaatct gttccttttt actgagccct
     6721 acgactctgg ctgagtgcca caccgccggc agccgcaaag cggctcaaac cattgccttt
     6781 tatggtaata atgagagaat gcagagggac ttcctttgtc tggcatatct gaggcgcgcg
     6841 ctgtcactag cgcccaccag cggtcagact gtagaatgca gagcaaaaca ggaagttgac
     6901 tccagatggt cacatgcttg ctgaaacgcc tctccctggc agcagtgcaa ttctaacagt
     6961 ttccctttct tttacagttc agcctcaagt ttgtacaaaa aagcaggctg gccgaagaag
     7021 aagcgcaaag tactggtacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
     7081 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     7141 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     7201 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     7261 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     7321 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     7381 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     7441 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     7501 gtctatatca tggccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac
     7561 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     7621 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     7681 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     7741 actctcggca tggacgagct gtacaagggc gaagggcaag ggcaagggca agggccgggc
     7801 cgcggctacg cgtatcgatc catgttcgag gcgcgcctgg tccagggctc catcctcaag
     7861 aaggtgttgg aggcactcaa ggacctcatc aacgaggcct gctgggatat tagctccagc
     7921 ggtgtaaacc tgcagagcat ggactcgtcc cacgtctctt tggtgcagct caccctgcgg
     7981 tctgagggct tcgacaccta ccgctgcgac cgcaacctgg ccatgggcgt gaacctcacc
     8041 agtatgtcca aaatactaaa atgcgccggc aatgaagata tcattacact aagggccgaa
     8101 gataacgcgg ataccttggc gctagtattt gaagcaccaa accaggagaa agtttcagac
     8161 tatgaaatga agttgatgga tttagatgtt gaacaacttg gaattccaga acaggagtac
     8221 agctgtgtag taaagatgcc ttctggtgaa tttgcacgta tatgccgaga tctcagccat
     8281 attggagatg ctgttgtaat ttcctgtgca aaagacggag tgaaattttc tgcaagtgga
     8341 gaacttggaa atggaaacat taaattgtca cagacaagta atgtcgataa agaggaggaa
     8401 gctgttacca tagagatgaa tgaaccagtt caactaactt ttgcactgag gtacctgaac
     8461 ttctttacaa aagccactcc actctcttca acggtgacac tcagtatgtc tgcagatgta
     8521 ccccttgttg tagagtataa aattgcggat atgggacact taaaatacta cttggctccc
     8581 aagatcgagg atgaagaagg atcttaggac ccagctttct tgtacaaagt gggggatcca
     8641 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     8701 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     8761 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     8821 gaggtttttt ccaactttat tatacatagt tgataattca ctggccgtcg ttttacggta
     8881 ccatcgatga tgatccagac atgataagat acattgatga gtttggacaa accacaacta
     8941 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     9001 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     9061 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     9121 ctgattatga tcctctagat cagatctgcg aagatacggc cacgggtgct cttgatcctg
     9181 tggctgattt tggactgtgc tgctcgcagc tgctgatgaa tcacatactt cctccatttt
     9241 cttccactga ttgactgtta taatttccct aatttccagg tcaaggtgct gtgcattgtg
     9301 gtaatagatg tgacatgacg tcacttccaa aggaccaatg aacatgtctg accaatttca
     9361 tataatgtga aaacgatttt cataggcaga ataaataaca tttaaattaa actgggcatc
     9421 agcgcaattc aattggtttg gtaatagcaa gggaaaatag aatgaagtga tctccaaaaa
     9481 ataagtactt tttgactgta aataaaattg taaggagtaa aaagtacttt tttttctaaa
     9541 aaaatgtaat taagtaaaag taaaagtatt gatttttaat tgtactcaag taaagtaaaa
     9601 atccccaaaa ataatactta agtacagtaa tcaagtaaaa ttactcaagt actttacacc
     9661 tctggttctt gaccccctac cttcagcaag cccagcagat ccactagttc tagagcggcc
     9721 gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     9781 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     9841 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     9901 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     9961 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    10021 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    10081 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    10141 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    10201 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    10261 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    10321 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    10381 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    10441 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    10501 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    10561 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    10621 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    10681 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    10741 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    10801 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa ggcattttgg tcatgagatt
    10861 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    10921 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    10981 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    11041 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    11101 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    11161 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    11221 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    11281 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    11341 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    11401 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    11461 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    11521 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    11581 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    11641 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    11701 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    11761 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    11821 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    11881 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    11941 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
    12001 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
    12061 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
    12121 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
    12181 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
    12241 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
    12301 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
    12361 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
    12421 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
    12481 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
    12541 aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtaccaaa
    12601 tagtaggaat tacccacctg tacaagtgct gaaaacttgg atgaataagc ccgtttgcca
    12661 ttttctactg ctattttaaa tcttttctgt atttgtctgc atttgtcttt acccttgaat
    12721 aaatgtttta gttgtttttt ttcaattatg actgtgttta acagacatta ttacaataat
    12781 atgtaatagt agtacacact attattatgt aatagtactt gttgactgta tttgagaact
    12841 ggaccgagtg agtgttacgt cacccattca aaatgactta cttctggctc caacaaaatg
    12901 aagttaattc agttgccatt tttcactgta tggacatcgc cgtgttggag ctagacgttg
    12961 ccaataagca agaaagagcc gagatgcgtc gagtctgagt caccgttcct atggcaaccc
    13021 ctctaaccaa tcagaagtaa gcttgttgga agtccacagc ctaccacttg aaagcgggct
    13081 gcacaaaatc tgtcaaacgt tttgaacgtt ggatgtgaga gcacatactt ttattaaggc
    13141 atctggttgg tcagtttata atatcaacaa cttgggctac agaaaagaaa agttattaca
    13201 gaaattatga ttaacaagta catgttaaat aaagatttta atatgaatgc caccactgga
    13261 gcattcatgc catttggagc ttcttcctgt ttggatcact agaaggagga ggtcactcat
    13321 tacagttctc atatacagtc gttggttggt tggttggttg gt
//
