LOCUS       sequence-203911-       13395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transgenesis, ubiquitous expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13395)
  AUTHORS   Daniel K, Icha J, Horenburg C, Muller D, Norden C, Mansfeld J
  TITLE     Conditional control of fluorescent protein degradation by an 
            auxin-dependent nanobody.
  JOURNAL   Nat Commun. 2018 Aug 17;9(1):3297. doi: 10.1038/s41467-018-05855-5.
  PUBMED    30120238
REFERENCE   2  (bases 1 to 13395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-08-17"; volume: "9(1):3297. doi"; pages: " 
            10.1038/s41467-018-05855-5"
COMMENT     Sequence Label: bactin:GFP-PCNA
FEATURES             Location/Qualifiers
     source          1..13395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(2535..2546,2547..2709)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     3082..3098
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3118..3138
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    8445..8469
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8472..8492
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      8505..8514
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8511..8513,8514..8516,8517..9227)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8556..8577)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8817..8836)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9164..9185
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(10069..10093)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    10095..10229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(10292..10312)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(10320..10336)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    10352..10486
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(10925..11112,11113..11124)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(11158..11174)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11158..11174)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(11211..11231)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11211..11229)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11250..11266)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11263..11285)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11274..11290
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11298..11304,11305..11322,11323..11328))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11343..11364
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11481..11498)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11652..12240)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11732..11751)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12411..13202,13203..13271))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13034..13053
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13272..13376)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccaaatagta ggaattaccc acctgtacaa gtgctgaaaa cttggatgaa taagcccgtt
      721 tgccattttc tactgctatt ttaaatcttt tctgtatttg tctgcatttg tctttaccct
      781 tgaataaatg ttttagttgt tttttttcaa ttatgactgt gtttaacaga cattattaca
      841 ataatatgta atagtagtac acactattat tatgtaatag tacttgttga ctgtatttga
      901 gaactggacc gagtgagtgt tacgtcaccc attcaaaatg acttacttct ggctccaaca
      961 aaatgaagtt aattcagttg ccatttttca ctgtatggac atcgccgtgt tggagctaga
     1021 cgttgccaat aagcaagaaa gagccgagat gcgtcgagtc tgagtcaccg ttcctatggc
     1081 aacccctcta accaatcaga agtaagcttg ttggaagtcc acagcctacc acttgaaagc
     1141 gggctgcaca aaatctgtca aacgttttga acgttggatg tgagagcaca tacttttatt
     1201 aaggcatctg gttggtcagt ttataatatc aacaacttgg gctacagaaa agaaaagtta
     1261 ttacagaaat tatgattaac aagtacatgt taaataaaga ttttaatatg aatgccacca
     1321 ctggagcatt catgccattt ggagcttctt cctgtttgga tcactagaag gaggaggtca
     1381 ctcattacag ttctcatata cagtcgttgg ttggttggtt ggttggttgg ttggtagatt
     1441 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
     1501 gattgattga ttgattgatt gattgattga ttgattgatt ggtagtcaaa ataagaaata
     1561 atttccacag attcattaca gaaatgatta aatgcataca taaaaaactg gggggggggg
     1621 ggatacaaca acacacttaa gtcacatttg cctacgtaaa gaaaagtaaa gaaaatcaat
     1681 agctatattt tacatctcct ttttttgctg tcttttaatt agccttgttt tgctgttatc
     1741 ttgattgaaa ctgtaacttc ttcacctgct tcttttcttt gtaggttttg ccagcccaga
     1801 aacaggcatg gctgtgcaag gccagagcac catgcacaat gcgctgcatg tcttcatgaa
     1861 cggctcaatg tcctcagtcc agggctcagc caacgacccc atcttcctcc ttcaccatgc
     1921 tttcattgac aggtaacaaa cacgtcatga cattagactg cacagttttt gacaaagttc
     1981 atacaatctg ttgtttatag ctgctacaat tagtgaagtt tgtgaatgta cttggatgag
     2041 cagcgaaaga tcaattgaga tcaattgtta gagtttggtt gccctgcaga gcaaagaaca
     2101 aaaaataatc tggtggcttt actgcgtgag gttattattg gtggaataga aacacaaaac
     2161 ataattgcat ttatttgttt aattttttat cttatcttaa ctttcatctt gcatatttgt
     2221 ttcttacatc atttctagca tctttgagcg ctggctaaga actcatcagc ctccccggtc
     2281 catctaccca cgtaccaatg caccaattgg ccacaatgac ggctactaca tggtgccatt
     2341 ccttcctctt tataggaatg gagactacct cctgtccaac aatgctcttg gatacgagta
     2401 cgcctacctg ttggacccag gtcattgcac aacaccagaa atgccctctg atctgcaaaa
     2461 gacgtgaata tctgttcaga cacccatatc cactctgttc cacacaggtc agaggtttgt
     2521 ccaggagttc ttgacagagg tgtaaaaagt actcaaaaat tttactcaag tgaaagtaca
     2581 agtacttagg gaaaatttta ctcaattaaa agtaaaagta tctggctaga atcttacttg
     2641 agtaaaagta aaaaagtact ccattaaaat tgtacttgag tattaaggaa gtaaaagtaa
     2701 aagcaagaaa gaaaactaga gattcttgtt taagctttta atctcaaaaa acattaaatg
     2761 aaatgcatac aaggttttat cctgctttag aactgtttgt atttaattat caaactataa
     2821 gacagacaat ctaatgccag tacacgctac tcaaagttgt aaaacctcag atttaacttc
     2881 agtagaagct gattctcaaa attgttagtg tcaagcctag ctcttttggg gctgaaaagc
     2941 aatcctgcag tgctgaaaag cctctcacag gcagccgatg cgggaagagg tgtattagtc
     3001 ttgatagaga ggctgcaaat agcaggaaac gtgagcagag actccctggt gtctgaaaca
     3061 caggccagat gggccctcga gcaggaaaca gctatgacca tgattacgcc aagctatcaa
     3121 ctttgtatag aaaagttgaa aattccagtt tgaagaaact tttcaagact caccttttca
     3181 ctgttggtct taatttaggt tgattatttt tgtcaattta ataattacat tgtattgttc
     3241 atattttatt gtcttattgt ggtgttttta cagctttttt attgttctgc tttgttactg
     3301 tttgccttgt atgcattgta gcgctttggg tctgagaaaa gcgcactata aataaactgt
     3361 attattatta ttagggatgg acatcacggt ggcgcgatgg gtggcacaat tacctcacag
     3421 caagtaggtt gctgtttaga gccccggctg gatcagttgg cagttctgtg tggagtttgc
     3481 atgttctccc tgttcaagta ggtttcctcc cggtgctctg gtttcccctc acaagtccaa
     3541 agacatgatg tataggtgaa tggggtaagc taaattgacc atagtgagtg tgtgtgtgtg
     3601 tgtgaatgag agtgtatagg tgtttcccag tgttaggttg caactggaag gacatctgct
     3661 gtgtaaaaca tatgcaggat aagttggcgg ttcattcccc tatggtgacc cctggttaat
     3721 taaaggacta agttgaaaat gaatgaatga atgaatgatt agagataaac cgataccact
     3781 tttttacaaa ccgatatgag tacaagtata atagtttctg ttactcgaca atactgagta
     3841 ccgattctga tacttcttgg atttggtttc aatgtaatta atttgaaaga aggttttatt
     3901 tcttgcctgt cgcaaggatg aacacaaaaa tctttaccag aaggatattt taagctaaga
     3961 atatacgcac gttgttgaca accctgtatc gattaccgat taaacgtgga catttaaagg
     4021 ataagttatg cttgcgccat cacaacttta cacattacat gatcaaagat catattgaat
     4081 attcggttta ttcgtcagaa gcctatagat ttgttaaaag tgtattttat aaacggatgg
     4141 tttataaaga ctaagctatt aatatatcag acattcatta atacatcaag acatcgatgt
     4201 caggtaggac aacaaatcac atccatgtaa tgaaatcttg ttgagtggta ttggtgcgtg
     4261 gtttcagcat ttcatgtgca atacgagtaa gaatatttta accaagtatc gacccaaaac
     4321 ctgatactgg tatcggtgca tcccttatta ttattattat caaaaacaca cttgttttat
     4381 ttatgtgggt gccaaacaac aaactatgaa atgcagcatg acacgcccaa aaagtgttcc
     4441 tttgagaaca acttcataaa tatgtttctt atcattatgc atgaatggtg tactgggaga
     4501 gctttggtat cagaattgag ataagagact ttggcattct aaaggacaat gacattatca
     4561 cactgacagt caacaacact ttattgctct ggaaatctta tgtcatgggg caaagctgag
     4621 gttcattagg acagtacctt atttagagat gaatgggaag gctgtagtgg gctgctatca
     4681 gggaagggtg gtggtcctcg ggggaactat ggactcacaa aagcacatct gctgtaacaa
     4741 gatcccctgt gcaatctaca aaagttacaa agagaagcat ggttggttaa aaaatagaat
     4801 tgactggata tgctgaatgc attgtttgtt gtgtgttaca tcacattaac cacagattgg
     4861 tgctaaatat ttgcttatct tataaaaacc tgtgtgttta taaagggggg tgtggatgga
     4921 tgcttggcat tctttgaaaa gatgttgggg gatggaagga ggagacctta ttgatgagta
     4981 tccagtttgc agataacaag tttttagaca ttcatactat gatgctgttt atgtctgttt
     5041 tttctcattt tttctataca caaacagcta taaaacattc acttttatat ctgatgctgt
     5101 atattaaatt tcgttgtatt tattctgttt gctctgttgg gtcatagcta gggccagaca
     5161 gaatctgctg acattttttg ctttttctgc agagaatttt gtaaacaatc tgtgatttat
     5221 gcgagatttt ttcgggagta tcgtaactaa aaacttacta tatgaaataa aaaaaaataa
     5281 taccttttta acttttaata aatatataca atgcaagtcc acctagatcc aattatttgg
     5341 tcgacaaagc aagtctctca cataatatat cttctaaaag acagaaaaca ttactttcag
     5401 accatattgt aatcaaatga acactttcat attagtcaat aatattacgg aaatgtattt
     5461 aaaaactgaa taaacatata tttacacctt tgtctttatt tctagagtgc ttttacaatg
     5521 tagattgtgt caaagcagct taacttagag gattagtata gtaaattgaa actgtgtcag
     5581 tccatttttt aagagtttaa gttcagttca gtttagttca gtgtgagtta atttttactg
     5641 ctgagagtcc aaacactaaa gagcaaatcc aaatacacaa gtcaataaat aaactcaatg
     5701 atgggctaaa gatctgggga aatctgcaga tttctgtgcg cacagattct gtgtgggcct
     5761 agtcattacc tatactcaac gtttactctg agaaacttcc atattttaga ggtaaaatgc
     5821 tcagaattgt aaaaagtctt gtcaaaatac ttggaatttg ccaaaaacaa aataaaagtc
     5881 acatcacctc attgtccttg gtatcagcaa gcacttttaa cacaatcagc tgtttttgtt
     5941 tataactccc actgaaggcc atgtgcatta ctcccaagtt acacagtaaa aagtcacatg
     6001 tagtttatca tgatcactgc tgctcaagca cagctttatc tgttattcag tgtggcaata
     6061 tctgaaatgt gtgcaaaata ttatttattg tagctgatca ccaacacttt cagtgttttt
     6121 tttttctact tttaatgaga attatgttct taggatttat ccttataaac ctctttaaaa
     6181 agaaatgtta tgtctgcata gacacgacat gaaattatga acagaaaatt taactattta
     6241 agatcaagac ttgacatata acatttctaa tgaacattaa acctgaggta ggcccacttg
     6301 caaaaatgta actgctatca ctcactttat acatcattgt gtaacttgta cacacaaaaa
     6361 tagtgaagca atgtggcatg gaatgcaggt cccattcatt caaagttatt aaagcacttt
     6421 cttaaagtgc attactactg agctaagacc agctagttgc tcatacaaca tactacaagt
     6481 agtgtcatcc taatttggat aaaaaaaatg tccattttat acatggaaaa gtactcatta
     6541 gatcccccat tgtttgtatt acggtatttc gtgaacacaa gaggtaaatg acctacagag
     6601 ctgctgttgt gttagattga aaacacaaca caggatcatg gaggcatcca tcattatgac
     6661 gcattctgcc acttgtaact ctgcacagac tttttgaaaa gtttaattga gtcttggcgt
     6721 tgtcgcagaa aaatacgaaa aattctgtgt taaaatttgc aaataaaaat tccagtttta
     6781 taggaaatcg ccatggcctt gttctttaac cagttaagct tccccttctt tcactctcaa
     6841 gttgcaagaa gcaagtgtag caatgtgcac gcgacagccg ggtgtgtgac gctggaccaa
     6901 tcagagcaca gagctccgaa agtttacctt ttatggctag agccgggtat gtgccgtcat
     6961 ataaaagagc gcgcccagct tttcagcctc actttgagct cctccacacg cagctagtgc
     7021 ggaatatcat ctgcttgtaa cccattctct taagtcgaca accccccaaa cccaaggtga
     7081 gttggtttta cagtttttaa gcgtttaatt atagtttcta tctattaatg ttaagtaaaa
     7141 tgtgtaaatg gataaatatg atttgatgaa gtctttagac agttcataac ggtctctttg
     7201 tagttatcta ctaggtttga tttataagtg gtggttcttt tgataatcta tgttacattt
     7261 aataaatatt ggctgtatct taactttttt gtagcgttag tggatgtggc aggtgagaat
     7321 gtcggtaatt taacgtgacc agtcgtaggc acgacattga acattgaata ggccggtgtg
     7381 aaatgggtgt taagtcttaa tataaagttg tgttctggct aatctctctt taacagccat
     7441 aaatgtgatt tttaactaca gttttgaggg tgctggtgag catgttgcac acttgatgga
     7501 tagccggcat gggaagttct ttgtgcaggc agtgctgcag cagggtgtga cctactttag
     7561 ctagccggct aaccagctct catctgatgt aacgtaaacc ccatcaatct aacatcggct
     7621 cattcgtgct ttataagatc gaagatcttg ttattagtaa cgggtattgg catttagttt
     7681 taacttggtt aattttcttt ttgttatgtg ttctatttaa atgtagcctg cagccccctc
     7741 tgtctttaat gaagctgaag attaacgtta acgttagtga agtgttgtat ccgctatgaa
     7801 agatttgaat tttagaggtg tggtccactt taataacggc tgcggagttc tctggctgca
     7861 cttcagccgc tgtgactgtc gaagcagagc ccatcagttt ttcaggatct cggtgatttt
     7921 tgaggctaga ctatgaactg aaccgactga gagtgaccgt ccagaaatgc tttagtgtga
     7981 agtctccgct cacgaggagt ccgtggactg actgcagatc tgtgacgcag taacacccgg
     8041 cggcagacac ccgttgaaat gcggttgtgt aattgatcgc aggcgagggt ggaagaggat
     8101 gtgaaacttc atttgtgtaa aatttaggga gtggtcctgg tgtgatgaat gtcgaaatct
     8161 gttccttttt actgagccct acgactctgg ctgagtgcca caccgccggc agccgcaaag
     8221 cggctcaaac cattgccttt tatggtaata atgagagaat gcagagggac ttcctttgtc
     8281 tggcatatct gaggcgcgcg ctgtcactag cgcccaccag cggtcagact gtagaatgca
     8341 gagcaaaaca ggaagttgac tccagatggt cacatgcttg ctgaaacgcc tctccctggc
     8401 agcagtgcaa ttctaacagt ttccctttct tttacagttc agcctcaagt ttgtacaaaa
     8461 aagcaggctg gccgaagaag aagcgcaaag tactggtacc ggtcgccacc atggtgagca
     8521 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     8581 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     8641 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     8701 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     8761 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     8821 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     8881 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     8941 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     9001 ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     9061 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     9121 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     9181 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggc gaagggcaag
     9241 ggcaagggca agggccgggc cgcggctacg cgtatcgatc catgttcgag gcgcgcctgg
     9301 tccagggctc catcctcaag aaggtgttgg aggcactcaa ggacctcatc aacgaggcct
     9361 gctgggatat tagctccagc ggtgtaaacc tgcagagcat ggactcgtcc cacgtctctt
     9421 tggtgcagct caccctgcgg tctgagggct tcgacaccta ccgctgcgac cgcaacctgg
     9481 ccatgggcgt gaacctcacc agtatgtcca aaatactaaa atgcgccggc aatgaagata
     9541 tcattacact aagggccgaa gataacgcgg ataccttggc gctagtattt gaagcaccaa
     9601 accaggagaa agtttcagac tatgaaatga agttgatgga tttagatgtt gaacaacttg
     9661 gaattccaga acaggagtac agctgtgtag taaagatgcc ttctggtgaa tttgcacgta
     9721 tatgccgaga tctcagccat attggagatg ctgttgtaat ttcctgtgca aaagacggag
     9781 tgaaattttc tgcaagtgga gaacttggaa atggaaacat taaattgtca cagacaagta
     9841 atgtcgataa agaggaggaa gctgttacca tagagatgaa tgaaccagtt caactaactt
     9901 ttgcactgag gtacctgaac ttctttacaa aagccactcc actctcttca acggtgacac
     9961 tcagtatgtc tgcagatgta ccccttgttg tagagtataa aattgcggat atgggacact
    10021 taaaatacta cttggctccc aagatcgagg atgaagaagg atcttaggac ccagctttct
    10081 tgtacaaagt gggggatcca gacatgataa gatacattga tgagtttgga caaaccacaa
    10141 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
    10201 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
    10261 aggttcaggg ggaggtgtgg gaggtttttt ccaactttat tatacatagt tgataattca
    10321 ctggccgtcg ttttacggta ccatcgatga tgatccagac atgataagat acattgatga
    10381 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
    10441 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
    10501 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
    10561 cctctacaaa tgtggtatgg ctgattatga tcctctagat cagatctgcg aagatacggc
    10621 cacgggtgct cttgatcctg tggctgattt tggactgtgc tgctcgcagc tgctgatgaa
    10681 tcacatactt cctccatttt cttccactga ttgactgtta taatttccct aatttccagg
    10741 tcaaggtgct gtgcattgtg gtaatagatg tgacatgacg tcacttccaa aggaccaatg
    10801 aacatgtctg accaatttca tataatgtga aaacgatttt cataggcaga ataaataaca
    10861 tttaaattaa actgggcatc agcgcaattc aattggtttg gtaatagcaa gggaaaatag
    10921 aatgaagtga tctccaaaaa ataagtactt tttgactgta aataaaattg taaggagtaa
    10981 aaagtacttt tttttctaaa aaaatgtaat taagtaaaag taaaagtatt gatttttaat
    11041 tgtactcaag taaagtaaaa atccccaaaa ataatactta agtacagtaa tcaagtaaaa
    11101 ttactcaagt actttacacc tctggttctt gaccccctac cttcagcaag cccagcagat
    11161 ccactagttc tagagcggcc gccaccgcgg tggagctcca gcttttgttc cctttagtga
    11221 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
    11281 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    11341 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    11401 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    11461 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    11521 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    11581 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    11641 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    11701 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    11761 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    11821 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    11881 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    11941 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    12001 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    12061 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
    12121 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    12181 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    12241 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    12301 ggcattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    12361 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    12421 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    12481 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    12541 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    12601 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    12661 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    12721 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    12781 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    12841 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    12901 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    12961 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    13021 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    13081 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    13141 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    13201 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    13261 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    13321 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    13381 tttccccgaa aagtg
//
