LOCUS       sequence-20398-e         629 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG414GPD-ccdB
FEATURES             Location/Qualifiers
     source          1..629
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(15..139)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(147..163)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(147..163)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(169..432)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(252..275)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
       61 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
      121 cttttttgta caaacttgtg atgggggatc cactagttct agaatccgtc gaaactaagt
      181 tctggtgttt taaaactaaa aaaaagacta actataaaag tagaatttaa gaagtttaag
      241 aaatagattt acagaattac aatcaatacc taccgtcttt atatacttat tagtcaagta
      301 ggggaataat ttcagggaac tggtttcaac cttttttttc agctttttcc aaatcagaga
      361 gagcagaagg taatagaagg tgtaagaaaa tgagatagat acatgcgtgg gtcaattgcc
      421 ttgtgtcatc atttactcca ggcaggttgc atcactccat tgaggttgtg cccgtttttt
      481 gcctgtttgt gcccctgttc tctgtagttg cgctaagaga atggacctat gaactgatgg
      541 ttggtgaaga aaacaatatt ttggtgctgg gattcttttt ttttctggat gccagcttaa
      601 aaagcgggct ccattatatt tagtggatg
//
