LOCUS       sequence-203995-       13089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mouse cDNA of HAL refractory to the guide in plasmid 
            4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13089)
  AUTHORS   Kanarek N, Keys HR, Cantor JR, Lewis CA, Chan SH, Kunchok T, 
            Abu-Remaileh M, Freinkman E, Schweitzer LD, Sabatini DM
  TITLE     Histidine catabolism is a major determinant of methotrexate 
            sensitivity.
  JOURNAL   Nature. 2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 
            10.1038/s41586-018-0316-7.
  PUBMED    29995852
REFERENCE   2  (bases 1 to 13089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 
            10.1038/s41586-018-0316-7."
COMMENT     Sequence Label: pWP1_mHAL
FEATURES             Location/Qualifiers
     source          1..13089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             131..764
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    811..936
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1429..1662
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1847..1891
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2274..3452
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2505..3443
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3400..3420
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3500..3617
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5652..6219
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5670..6221
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5814..5831)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6041..6060
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6224..6226,6227..6229,6230..6940)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6224..6226,6227..6229,6230..6940)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6269..6290)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6530..6549)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6877..6898
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7019..7035)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7020..7036)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    7049..7637
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7102..7122)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7520..7531)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7781..7814)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7834..8014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8039..8057)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8040..8057)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8347..8366)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8580..8599)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8699..8721
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8759..8777)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8845..8949
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8950..9018,9019..9810)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9168..9187)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9981..10569
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10470..10489
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10723..10740
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10810..10830)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10815..11144
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10995..11130
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12278..12343
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12473..12493
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12918..13052
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12955..12974)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13009..13028
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
       61 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
      121 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
      181 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
      241 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
      301 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
      361 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
      421 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
      481 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
      541 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
      601 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
      661 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
      721 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
      781 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
      841 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
      901 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
      961 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     1021 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     1081 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     1141 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     1201 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     1261 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     1321 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     1381 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     1441 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     1501 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     1561 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     1621 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     1681 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     1741 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     1801 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     1861 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     1921 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     1981 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     2041 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     2101 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga
     2161 ctagcctcga ccatcgatgt cgacgataag ctttgcaaag atggataaag ttttaaacag
     2221 agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg
     2281 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     2341 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     2401 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     2461 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
     2521 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
     2581 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
     2641 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
     2701 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
     2761 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
     2821 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
     2881 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
     2941 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
     3001 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     3061 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
     3121 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     3181 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     3241 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     3301 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     3361 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     3421 aaagtttttt tcttccattt caggtgtcgt gaggaatttc gacatttaaa tttaattaat
     3481 ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg
     3541 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     3601 caaaaattca aaattttatc gatcacgaga ctagcctcga ggtttctcga ggccaccatg
     3661 cctaggtaca cagtgcacgt gcgtggggaa tggctggcgg tgccctgtca ggatgggaag
     3721 ctcactgttg gctggctggg ccgtgaggct gtgcgacgct acatgaagaa caaaccggac
     3781 aatggtggct tcacttcggt ggatgaagtg caattccttg tgcatcggtg caagggcctg
     3841 ggcctgctgg acaatgagga cgagctggag gtggccttag aggacaatga gttcgtagaa
     3901 gtggtgattg aaggtgatgt gatgtctcct gatttcattc cgtcgcagcc agaaggagtt
     3961 ttcctataca gcaaataccg ggagcctgaa aaatacattg ccctagatgg ggacagtctg
     4021 agcacagagg atctggtcaa cctgggcaag ggacgttaca agataaagct cacttcaatt
     4081 gctgagaaga aagtgcagca gtcccgggag gtcatcgaca gcatcatcaa agaaagaaca
     4141 gttgtttatg gcatcaccac aggttttgga aaatttgcta gaactgtaat ccctgccaat
     4201 aagctacagg agcttcaagt caacttagtc cgttcccatt cttcaggtgt tgggaaacca
     4261 ctcagtcctg agaggtgtcg gatgctcttg gctttgagga tcaacgtctt agccaaaggt
     4321 tacagtggga tttccttgga gacgctgaaa caagttattg aagcatttaa tgcctcctgc
     4381 ctgtcctatg ttccagagaa aggaactgtg ggtgccagcg gagaccttgc cccactctct
     4441 catcttgctc tggggctcat cggagaagga aagatgtggt ccccgaagag tggctgggca
     4501 gatgctaaat atgttctaga agcccatgga ctgaaaccaa ttgtcttgaa accaaaagaa
     4561 ggcctggcac tcatcaatgg gacacagatg atcacttccc tgggctgtga agccctggag
     4621 cgagccagcg ccattgcccg gcaagctgat attgtggctg ccttgaccct ggaggtgctg
     4681 aagggcacca ccaaagcctt cgataccgac atccatgctg tccgacctca ccgtgggcaa
     4741 attgaagttg ctttccgatt ccggtccctc ctggactcgg atcaccaccc atcagaaatc
     4801 gcagaaagcc acaggttctg cgatcgtgtt caggacgcgt acaccttacg ctgctgtcca
     4861 caggtccacg gtgtggtgaa cgacacaata gcctttgtga aggacatcat cactacagaa
     4921 ctgaacagcg ccacagacaa tcctatggtc tttgccagtc gaggggagac aatttcagga
     4981 ggaaacttcc atggtgaata cccagccaaa gccctggact atttggccat tggtgtccat
     5041 gaacttgcgg caattagtga aagaagaatc gaaaggctgt gtaatccttc cctcagcgag
     5101 ctgcctgcct tcctggtggc tgaaggaggt ctcaattctg ggttcatgat agcccactgc
     5161 accgcagcag ccctggtgtc tgagagcaag gctctgtgcc acccttcatc tgtggactca
     5221 ctctcgacca gtgctgctac agaagaccac gtctccatgg gaggatgggc agccaggaaa
     5281 gccctcaggg tcgtcgagca cgtggaacaa gtgctagcca ttgagcttct cgcggcctgc
     5341 cagggtatag agtttctgcg ccccctgaaa acaaccactc cgctggagaa ggtgtacgac
     5401 ctggtgcgct ccgtagtaag gccctggata aaagatcgct tcatggcccc ggacattgag
     5461 gcagcacaca ggttgcttct tgaccaaaag gtttgggagg ttgccgcacc gtacatcgag
     5521 aaatacagaa tggagcacat tccggaatcc agaccgcttt ctccaacagc gttttcacta
     5581 gaatctctcc gaaagaactc agccacaatc ccagagtctg acgatcttta aaaactacgg
     5641 gctgcaggaa ttccgccccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     5701 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     5761 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     5821 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     5881 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     5941 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     6001 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     6061 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     6121 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac
     6181 cacggggacg tggttttcct ttgaaaaaca cgatgataat accatggtga gcaagggcga
     6241 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     6301 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     6361 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     6421 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     6481 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     6541 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     6601 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     6661 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     6721 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     6781 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     6841 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     6901 cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca gatctcgact
     6961 agctagtagc tagctagcta gtcgagctca agcttcgaat tcgatatcaa gcttatcgcg
     7021 ataccgtcga cctcgaggga attccgataa tcaacctctg gattacaaaa tttgtgaaag
     7081 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     7141 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     7201 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     7261 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     7321 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     7381 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     7441 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     7501 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     7561 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     7621 ttgggccgcc tccccgcatc gggaattcga gctcggtacc tttaagacca atgacttaca
     7681 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     7741 actcccaacg aagacaagat gggatcaatt caccatggga ataacttcgt atagcataca
     7801 ttatacgaag ttatgctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     7861 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7921 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7981 agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc
     8041 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
     8101 gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc
     8161 tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt
     8221 ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta
     8281 gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
     8341 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
     8401 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
     8461 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
     8521 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt
     8581 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     8641 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     8701 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     8761 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     8821 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     8881 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     8941 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     9001 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     9061 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     9121 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     9181 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     9241 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     9301 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     9361 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     9421 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     9481 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     9541 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     9601 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     9661 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     9721 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     9781 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     9841 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     9901 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9961 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    10021 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    10081 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
    10141 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    10201 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    10261 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    10321 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    10381 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    10441 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    10501 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    10561 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    10621 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    10681 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    10741 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
    10801 tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
    10861 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
    10921 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
    10981 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    11041 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    11101 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga
    11161 caggcttgcg agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg
    11221 taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc
    11281 gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt
    11341 cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc
    11401 gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt
    11461 ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
    11521 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
    11581 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga
    11641 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
    11701 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg
    11761 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac
    11821 tgccgggcgt tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg
    11881 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
    11941 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag
    12001 tcggcccttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
    12061 atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
    12121 gatgccgaac gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac
    12181 tggatttatg agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata
    12241 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
    12301 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
    12361 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
    12421 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
    12481 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
    12541 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
    12601 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
    12661 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
    12721 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
    12781 ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
    12841 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
    12901 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    12961 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    13021 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
    13081 atcccaaac
//
