LOCUS       sequence-204-exp        4163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3787
FEATURES             Location/Qualifiers
     source          1..4163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(534..562)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1310..1330
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        1908..2012
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2013..2081,2082..2873)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2231..2250)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3044..3632
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3533..3552
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3786..3803
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3920..3941
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3956..3961,3962..3979,3980..3986)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3994..4010
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3999..4021
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4018..4034
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4018..4034
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatcaagag catttgaatc agaatatgga
       61 gaacggagca tgagcatttt cgaagttttt tagatgcact agaacaaagc gtgttggctt
      121 cctctgagcc cgctttcctt atatacccgc attctgcagc cttacagaat gttctagaag
      181 gtcctagatg cattcgtttg aaaatactcc cggtgggtgc aaagagacgc agacggaaaa
      241 tgtatctggg tctctttatt gtgtacacta cttttccatg taccgaatgt gagtcgccct
      301 ccttttgcaa caagcagctc gaatgttcta gaaaaaggtg gaaaatagta taaataccgt
      361 tgaaaataaa taccgaacaa catttgctct aattgtgaaa ttagaaatct tcaaactata
      421 atcatctcac tggatcctat cgattcgcgg ccgctgtaca cccgggtgcc ctatagtgag
      481 tcgtattggt accggtagaa aaaatgagta aaggagaaga acttttcact ggagttgtcc
      541 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
      601 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
      661 tacctgttcc atgggtaagt ttaaacatat atatactaac taaccctgat tatttaaatt
      721 ttcagccaac acttgtcact actttctgtt atggtgttca atgcttctcg agatacccag
      781 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
      841 gaactatatt tttcaaagat gacgggaact acaagacacg taagtttaaa cagttcggta
      901 ctaactaacc atacatattt aaattttcag gtgctgaagt caagtttgaa ggtgataccc
      961 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
     1021 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga
     1081 atggaatcaa agttgtaagt ttaaacatga ttttactaac taactaatct gatttaaatt
     1141 ttcagaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt
     1201 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
     1261 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg
     1321 agtttgtaac agctgctggg attacacatg gcatggacga actatacaaa agcccacaag
     1381 ctttggaatt cgctagccgg ccatacaagt aatccggatg atcgacgcca acgtcgttga
     1441 attttcaaat tttaaatact gaatatttgt tttttttcct attatttatt tattctcttt
     1501 gtgttttttt tcttgctttc taaaaaatta attcaatcca aatctaaaca tttttttttc
     1561 tctttccgtc tcccaattcg tattccgctc ctctcatctg aacacaatgt gcaagtttat
     1621 ttatcttctc gctttcattt cattaggacg tggggggaat tggtggaagg gggaaacaca
     1681 caaaaggatg atggaaatga aataaggaca cacaatatgc aacaacattc aattcagaaa
     1741 tatggaggaa ggtttaaaag aaaacataaa aatatataga ggaggaagga aaactagtaa
     1801 aaaataagca aagaaattag gcgaacgatg agaattgtcc tcgcttgggc ccaaaaggcc
     1861 tttttttttt ggcgccgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1921 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1981 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     2041 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     2101 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     2161 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     2221 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     2281 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     2341 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2401 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     2461 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     2521 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     2581 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     2641 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     2701 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     2761 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     2821 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     2881 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2941 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3001 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3061 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3121 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3181 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3241 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3301 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3361 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3421 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3481 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3541 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3601 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3661 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3721 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3781 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     3841 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     3901 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     3961 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     4021 gaaacagcta tgaccatgat tacgccaagc tgtaagttta aacatgatct tactaactaa
     4081 ctattctcat ttaaattttc agagcttaaa aatggctgaa atcactcaca acgatggata
     4141 cgctaacaac ttggaaatga aat
//
