LOCUS       sequence-204017-         234 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary vectors used for the constitutive expression of tripartite 
            split-sfGFP components (with a 3xHA tag) in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 234)
  AUTHORS   Liu TY, Chou WC, Chen WY, Chu CY, Dai CY, Wu PY
  TITLE     Detection of membrane protein-protein interaction in planta based on
            dual intein-coupled tripartite split-GFP association.
  JOURNAL   Plant J. 2018 Feb 16. doi: 10.1111/tpj.13874.
  PUBMED    29451720
REFERENCE   2  (bases 1 to 234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2018 Feb 16. doi: 10.1111/tpj.13874."
COMMENT     Sequence Label: P16-Delta-S:S10-(MCS)-3xHA
FEATURES             Location/Qualifiers
     source          1..234
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             136..225
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 ggatccatgg atttaccaga cgaccattac ctgtcaacac aaactatcct ttcgaaagat
       61 ctcaacggta cggacgttgg gtctggcggt ggctccggcg cgcccgggtt aattaactcg
      121 agtctagagt cgacttaccc atacgatgtt ccagattacg ctggctaccc atacgatgtt
      181 ccagattacg ctggatcata cccatacgat gttccagatt acgctactag ctaa
//
