LOCUS       sequence-204030-        6759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HLA-A0201 his tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Lampen MH, Uchtenhagen H, Blom K, Varnaite R, Pakalniene J, 
            Dailidyte L, Walchli S, Lindquist L, Mickiene A, Michaelsson J, 
            Schumacher TN, Ljunggren HG, Sandberg JK, Achour A, Gredmark-Russ S
  TITLE     Breadth and Dynamics of HLA-A2- and HLA-B7-Restricted CD8(+) T Cell 
            Responses against Nonstructural Viral Proteins in Acute Human 
            Tick-Borne Encephalitis Virus Infection.
  JOURNAL   Immunohorizons. 2018 Jul 2;2(6):172-184. doi: 
            10.4049/immunohorizons.1800029.
  PUBMED    31022685
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/immunohorizons"; journalName: "Immunohorizons"; date: 
            "2018-07-2- 2"; volume: "2"; issue: "6"; pages: "172-184"
COMMENT     Sequence Label: pMP71-HLA-A0201-His
FEATURES             Location/Qualifiers
     source          1..6759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1196..1213
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(1239..1263)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1508..1911
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             2418..3008
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3033..3049)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3033..3049)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3046..3068)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3057..3073
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3081..3087,3088..3105,3106..3111))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3126..3147
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3264..3281)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3435..4023)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3515..3534)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4194..4985,4986..5054))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4817..4836
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5055..5159)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5227..5245
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5283..5305)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5405..5424
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5618..5640
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5632..5649
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5633..5649
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgagtaata cgactcacta tagggagacc caagctggct aggtaagctt gatcaacaag
       61 tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atggccgtca tggcgccccg
      121 aaccctcgtc ctgctactct cgggggctct ggccctgacc cagacctggg cgggctctca
      181 ctccatgagg tatttcttca catccgtgtc ccggcccggc cgcggggagc cccgcttcat
      241 cgcagtgggc tacgtggacg acacgcagtt cgtgcggttc gacagcgacg ccgcgagcca
      301 gaggatggag ccgcgggcgc cgtggataga gcaggagggt ccggagtatt gggacgggga
      361 gacacggaaa gtgaaggccc actcacagac tcaccgagtg gacctgggga ccctgcgcgg
      421 ctactacaac cagagcgagg ccggttctca caccgtccag aggatgtatg gctgcgacgt
      481 ggggtcggac tggcgcttcc tccgcgggta ccaccagtac gcctacgacg gcaaggatta
      541 catcgccctg aaagaggacc tgcgctcttg gaccgcggcg gacatggcag ctcagaccac
      601 caagcacaag tgggaggcgg cccatgtggc ggagcagttg agagcctacc tggagggcac
      661 gtgcgtggag tggctccgca gatacctgga gaacgggaag gagacgctgc agcgcacgga
      721 cgcccccaaa acgcatatga ctcaccacgc tgtctctgac catgaagcca ccctgaggtg
      781 ctgggccctg agcttctacc ctgcggagat cacactgacc tggcagcggg atggggagga
      841 ccagacccag gacacggagc tcgtggagac caggcctgca ggggatggaa ccttccagaa
      901 gtgggcggct gtggtggtgc cttctggaca ggagcagaga tacacctgcc atgtgcagca
      961 tgagggtttg cccaagcccc tcaccctgag atgggagccg tcttcccagc ccaccatccc
     1021 catcgtgggc atcattgctg gcctggttct ctttggagct gtgatcactg gagctgtggt
     1081 cgctgctgtg atgtggagga ggaagagctc agatagaaaa ggagggagct actctcaggc
     1141 tgcaagcagt gacagtgccc agggctctga tgtgtctctc acagcttgta aagtgcatca
     1201 ccatcaccat cactgactcg agaagggtgg gcgcgccgac ccagctttct tgtacaaagt
     1261 ggttgatcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc aattcgagca
     1321 tcttaccgcc atttattccc atatttgttc tgtttttctt gatttgggta tacatttaaa
     1381 tgttaataaa acaaaatggt ggggcaatca tttacatttt atgggatatg taattactag
     1441 ttcaggtgta ttgccacaag acaaacatgt taagaaactt tcccgttatt tacgctctgt
     1501 tcctgttaat caacctctgg attacaaaat ttgtgaaaga ttgactgata ttcttaacta
     1561 tgttgctcct tttacgctgt gtggatatgc tgctttaatg cctctgtatc atgctattgc
     1621 ttcccgtacg gctttcgttt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     1681 ggagttgtgg cccgttgtcc gtcaacgtgg cgtggtgtgc tctgtgtttg ctgacgcaac
     1741 ccccactggc tggggcattg ccaccacctg tcaactcctt tctgggactt tcgctttccc
     1801 cctcccgatc gccacggcag aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     1861 taggttgctg ggcactgata attccgtggt gttgtcgggg aagctgacgt cctttccatg
     1921 gctgctcgcc tgtgttgcca actggatcct gcgcgggacg tccttctgct acgtcccttc
     1981 ggctctcaat ccagcggacc tcccttcccg aggccttctg ccggttctgc ggcctctccc
     2041 gcgtcttcgc tttcggcctc cgacgagtcg gatctccctt tgggccgcct ccccgcctgt
     2101 ttcgcctcgg cgtccggtcc gtgttgcttg gtcgtcacct gtgcagaatt gcgaaccatg
     2161 gattccaccg tgaactttgt ctcctggcat gcaaatcgtc aacttggcat gccaagaatt
     2221 aattcggatc caagcttagg cctgctcgct ttcttgctgt cccatttcta ttaaaggttc
     2281 ctttgttccc taagtccaac tactaaactg ggggatatta tgaagggcct tgagcatctg
     2341 gattctgcct agcgctaagc ttaacacgag ccatagatag aataaaagat tttatttagt
     2401 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta
     2461 acgccatttt gcaaggcatg gaaaatacat aactgagaat agagaagttc agatcaaggt
     2521 taggaacaga gagacagcag aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     2581 ccccggctca gggccaagaa cagttggaac agcagaatat gggccaaaca ggatatctgt
     2641 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtcccg
     2701 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
     2761 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
     2821 tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat
     2881 agactgcgtc gcccgggtac ccgtgttctc aataaaccct cttgcagttg catccgactc
     2941 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactgccca cctcgggggt
     3001 ctttcattct cgagcagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3061 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3121 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3181 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3241 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3301 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3361 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3421 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3481 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3541 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3601 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3661 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3721 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3781 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3841 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3901 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3961 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4021 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4081 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     4141 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     4201 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     4261 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     4321 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4381 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     4441 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     4501 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     4561 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     4621 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     4681 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     4741 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     4801 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4861 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4921 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     4981 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5041 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5101 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5161 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     5221 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     5281 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     5341 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     5401 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     5461 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     5521 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     5581 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     5641 acggccagtg aattagtact ctagcttaag taacgccatt ttgcaaggca tggaaaatac
     5701 ataactgaga atagagaagt tcagatcaag gttaggaaca gagagacagc agaatatggg
     5761 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagttgga
     5821 acagcagaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     5881 ccaagaacag atggtcccca gatgcggtcc cgccctcagc agtttctaga gaaccatcag
     5941 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat
     6001 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca
     6061 caacccctca ctcggcgcgc cagtcctccg atagactgcg tcgcccgggt acccgtattc
     6121 ccaataaagc ctcttgctgt ttgcatccga atcgtggact cgctgatcct tgggagggtc
     6181 tcctcagatt gattgactgc ccacctcggg ggtctttcat ttggaggttc caccgagatt
     6241 tggagacccc tgcccaggga ccaccgaccc ccccgccggg aggtaagctg gccagcggtc
     6301 gtttcgtgtc tgtctctgtc tttgtgcgtg tttgtgccgg catctaatgt ttgcgcctgc
     6361 gtctgtacta gttggctaac tagatctgta tctggcggtc ccgcggaaga actgacgagt
     6421 tcgtattccc ggccgcagcc cctgggagac gtcccagcgg cctcgggggc ccgttttgtg
     6481 gcccattctg tatcagttaa cctacccgag tcggactttt tggagctccg ccactgtccg
     6541 aggggtacgt ggctttgttg ggggacgaga gacagagaca cttcccgccc ccgtctgaat
     6601 ttttgctttc ggttttacgc cgaaaccgcg ccgcgcgtct tgtctgctgc agcatcgttc
     6661 tgtgttgtct ctgtctgact gtgtttctgt atttgtctga aaattagctc gacaaagtta
     6721 agtaatagtc cctctctcca agctcactta caggcggcc
//
