LOCUS       sequence-204032-        6408 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression vector for fusion of Gal4 DNA binding domain to 
            estrogen receptor alpha hormone binding domain to VP16 
            transcriptional activation domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Louvion JF, Havaux-Copf B, Picard D
  TITLE     Fusion of GAL4-VP16 to a steroid-binding domain provides a tool for 
            gratuitous induction of galactose-responsive genes in yeast.
  JOURNAL   Gene 1993 Sep 6;131(1):129-34.
  PUBMED    8370533
REFERENCE   2  (bases 1 to 6408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1993-09-6"; volume: "131(1)"; pages: "129-34"
COMMENT     Sequence Label: pHCA/GAL4(1-93).ER.VP16
FEATURES             Location/Qualifiers
     source          1..6408
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..27
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     12..28
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        814..1518
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     primer_bind     complement(1551..1570)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1552..1570)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1577..1594)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1577..1593)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1586..1608)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1738..2193
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1825..1844)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2035..2056
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(2454..3113)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(3114..3301)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(3446..3465)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3565..3587
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3625..3643)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    3685..4188
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        4225..4329
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4330..4398,4399..5190)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4548..4567)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5361..5949
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6103..6120
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(6273..6278,6279..6296,6297..6303)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6311..6327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6316..6338
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6335..6351
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6335..6351
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        6372..6390
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagcccggg
       61 ggatccggga tcgaagaaat gatggtaaat gaaataggaa atcaaggagc atgaaggcaa
      121 aagacaaata taagggtcga acgaaaaata aagtgaaaag tgttgatatg atgtatttgg
      181 ctttgcggcg ccgaaaaaac gagtttacgc aattgcacaa tcatgctgac tctgtggcgg
      241 acccgcgctc ttgccggccc ggcgataacg ctgggcgtga ggctgtgccc ggcggagttt
      301 tttgcgcctg cattttccaa ggtttaccct gcgctaaggg gcgagattgg agaagcaata
      361 agaatgccgg ttggggttgc gatgatgacg accacgacaa ctggtgtcat tatttaagtt
      421 gccgaaagaa cctgagtgca tttgcaacat gagtatacta gaagaatgag ccaagacttg
      481 cgagacgcga gtttgccggt ggtgcgaaca atagagcgac catgaccttg aaggtgagac
      541 gcgcataacc gctagagtac tttgaagagg aaacagcaat agggttgcta ccagtataaa
      601 tagacaggta catacaacac tggaaatggt tgtctgtttg agtacgcttt caattcattt
      661 gggtgtgcac tttattatgt tacaatatgg aagggaactt tacacttctc ctatgcacat
      721 atattaatta aagtccaatg ctagtagaga aggggggtaa cacccctccg cgctcttttc
      781 cgattttttt ctaaaccgtg gaatatttcg gatatccttt tgttgtttcc gggtgtacaa
      841 tatggacttc ctcttttctg gcaaccaaac ccatacatcg ggattcctat aataccttcg
      901 ttggtctccc taacatgtag gtggcggagg ggagatatac aatagaacag ataccagaca
      961 agacataatg ggctaaacaa gactacacca attacactgc ctcattgatg gtggtacata
     1021 acgaactaat actgtagccc tagacttgat agccatcatc atatcgaagt ttcactaccc
     1081 tttttccatt tgccatctat tgaagtaata ataggcgcat gcaacttctt ttcttttttt
     1141 ttcttttctc tctcccccgt tgttgtctca ccatatccgc aatgacaaaa aaatgatgga
     1201 agacactaaa ggaaaaaatt aacgacaaag acagcaccaa cagatgtcgt tgttccagag
     1261 ctgatgaggg gtatctcgaa gcacacgaaa ctttttcctt ccttcattca cgcacactac
     1321 tctctaatga gcaacggtat acggccttcc ttccagttac ttgaatttga aataaaaaaa
     1381 agtttgctgt cttgctatca agtataaata gacctgcaat tattaatctt ttgtttcctc
     1441 gtcattgttc tcgttccctt tcttccttgt ttctttttct gcacaatatt tcaagctata
     1501 ccaagcatac aatcaactcc aagctttttt tttttttgag ctccaattcg ccctatagtg
     1561 agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     1621 ttacccaact taatcgcctt gcagcacatc cccccttcgc cagctggcgt aatagcgaag
     1681 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg
     1741 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     1801 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     1861 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     1921 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     1981 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     2041 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     2101 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     2161 cgaattttaa caaaatatta acgtttacaa tttcctgatg cggtattttc tccttacgca
     2221 tctgtgcggt atttcacacc gcatatgatc cgtcgagttc aagagaaaaa aaaagaaaaa
     2281 gcaaaaagaa aaaaggaaag cgcgcctcgt tcagaatgac acgtatagaa tgatgcatta
     2341 ccttgtcatc ttcagtatca tactgttcgt atacatactt actgacattc ataggtatac
     2401 atatatacac atgtatatat atcgtatgct gcagctttaa ataatcggtg tcactacata
     2461 agaacacctt tggtggaggg aacatcgttg gtaccattgg gcgaggtggc ttctcttatg
     2521 gcaaccgcaa gagccttgaa cgcactctca ctacggtgat gatcattctt gcctcgcaga
     2581 caatcaacgt ggagggtaat tctgctagcc tctgcaaagc tttcaagaaa atgcgggatc
     2641 atctcgcaag agagatctcc tactttctcc ctttgcaaac caagttcgac aactgcgtac
     2701 ggcctgttcg aaagatctac caccgctctg gaaagtgcct catccaaagg cgcaaatcct
     2761 gatccaaacc tttttactcc acgcgccagt agggcctctt taaaagcttg accgagagca
     2821 atcccgcagt cttcagtggt gtgatggtcg tctatgtgta agtcaccaat gcactcaacg
     2881 attagcgacc agccggaatg cttggccaga gcatgtatca tatggtccag aaaccctata
     2941 cctgtgtgga cgttaatcac ttgcgattgt gtggcctgtt ctgctactgc ttctgcctct
     3001 ttttctggga agatcgagtg ctctatcgct aggggaccac cctttaaaga gatcgcaatc
     3061 tgaatcttgg tttcatttgt aatacgcttt actagggctt tctgctctgt catctttgcc
     3121 ttcgtttatc ttgcctgctc attttttagt atattcttcg aagaaatcac attactttat
     3181 ataatgtata attcattatg tgataatgcc aatcgctaag aaaaaaaaag agtcatccgc
     3241 taggtggaaa aaaaaaaatg aaaatcatta ccgaggcata aaaaaatata gagtgtacta
     3301 gaggaggcca agagtaatag aaaaagaaaa ttgcgggaaa ggactgtgtt atgacttccc
     3361 tgactaatgc cgtgttcaaa cgatacctgg cagtgactcc tagcgctcac caagctctta
     3421 aaacggaatt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     3481 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     3541 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     3601 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     3661 tgataataat ggtttcttag gacggatcgc ttgcctgtaa cttacacgcg cctcgtatct
     3721 tttaatgatg gaataatttg ggaatttact ctgtgtttat ttatttttat gttttgtatt
     3781 tggattttag aaagtaaata aagaaggtag aagagttacg gaatgaagaa aaaaaaataa
     3841 acaaaggttt aaaaaatttc aacaaaaagc gtactttaca tatatattta ttagacaaga
     3901 aaagcagatt aaatagatat acattcgatt aacgataagt aaaatgtaaa atcacaggat
     3961 tttcgtgtgt ggtcttctac acagacaaga tgaaacaatt cggcattaat acctgagagc
     4021 aggaagagca agataaaagg tagtatttgt tggcgatccc cctagagtct tttacatctt
     4081 cggaaaacaa aaactatttt ttctttaatt tcttttttta ctttctattt ttaatttata
     4141 tatttatatt aaaaaattta aattataatt atttttatag cacgtgatga aaaggaccca
     4201 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4261 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4321 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4381 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4441 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4501 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4561 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4621 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     4681 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     4741 acaacgatcg gaggaccgaa ggagctaacc gctttttttc acaacatggg ggatcatgta
     4801 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     4861 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     4921 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4981 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5041 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5101 gttatctaca cgacgggcag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5161 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5221 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5281 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5341 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5401 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5461 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     5521 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5581 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5641 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5701 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa
     5761 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5821 acaggagagc gcacgaggga gcttccaggg gggaacgcct ggtatcttta tagtcctgtc
     5881 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggccgagc
     5941 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6001 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6061 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6121 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     6181 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     6241 gtgagttacc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcctatg
     6301 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     6361 gccaagctcg gaattaaccc tcactaaagg gaacaaaagc tgggtacc
//
