LOCUS       sequence-204035-        6857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for mCherry fusion to human Hsp90 beta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Picard D, Suslova E, Briand PA
  TITLE     2-color photobleaching experiments reveal distinct intracellular 
            dynamics of two components of the Hsp90 complex.
  JOURNAL   Exp Cell Res. 2006 Nov 15. 312(19):3949-58.
  PUBMED    17010336
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp Cell 
            Res. 2006 Nov 15. 312(19):3949-58."
COMMENT     Sequence Label: pCherry.90 beta
FEATURES             Location/Qualifiers
     source          1..6857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      595..604
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             601..1308
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(746..764)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1014..1033
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3645..3766
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3682..3701)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3736..3755
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3773..4228)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3910..3931)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4122..4141
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4255..4359
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4361..4718
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4361..4552
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4384..4404)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4569..4704
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4753..5547
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4753..5547
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4807..4826)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5417..5436
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5735..5754)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5779..5826
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6155..6743
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6644..6663
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc
      601 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
      661 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      721 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      781 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      841 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
      901 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      961 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     1021 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     1081 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     1141 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     1201 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     1261 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagag atctggaggt
     1321 atgcctgagg aagtgcacca tggagaggag gaggtggaga cttttgcctt tcaggcagaa
     1381 attgcccaac tcatgtccct catcatcaat accttctatt ccaacaagga gattttcctt
     1441 cgggagttga tctctaatgc ttctgatgcc ttggacaaga ttcgctatga gagcctgaca
     1501 gacccttcga agttggacag tggtaaagag ctgaaaattg acatcatccc caaccctcag
     1561 gaacgtaccc tgactttggt agacacaggc attggcatga ccaaagctga tctcataaat
     1621 aatttgggaa ccattgccaa gtctggtact aaagcattca tggaggctct tcaggctggt
     1681 gcagacatct ccatgattgg gcagtttggt gttggctttt attctgccta cttggtggca
     1741 gagaaagtgg ttgtgatcac aaagcacaac gatgatgaac agtatgcttg ggagtcttct
     1801 gctggaggtt ccttcactgt gcgtgctgac catggtgagc ccattggcag gggtaccaaa
     1861 gtgatcctcc atcttaaaga agatcagaca gagtacctag aagagaggcg ggtcaaagaa
     1921 gtagtgaaga agcattctca gttcataggc tatcccatca ccctttattt ggagaaggaa
     1981 cgagagaagg aaattagtga tgatgaggca gaggaagaga aaggtgagaa agaagaggaa
     2041 gataaagatg atgaagaaaa acccaagatc gaagatgtgg gttcagatga ggaggatgac
     2101 agcggtaagg ataagaagaa gaaaactaag aagatcaaag agaaatacat tgatcaggaa
     2161 gaactaaaca agaccaagcc tatttggacc agaaaccctg atgacatcac ccaagaggag
     2221 tatggagaat tctacaagag cctcactaat gactgggaag accacttggc agtcaagcac
     2281 ttttctgtag aaggtcagtt ggaattcagg gcattgctat ttattcctcg tcgggctccc
     2341 tttgaccttt ttgagaacaa gaagaaaaag aacaacatca aactctatgt ccgccgtgtg
     2401 ttcatcatgg acagctgtga tgagttgata ccagagtatc tcaattttat ccgtggtgtg
     2461 gttgactctg aggatctgcc cctgaacatc tcccgagaaa tgctccagca gagcaaaatc
     2521 ttgaaagtca ttcgcaaaaa cattgttaag aagtgccttg agctcttctc tgagctggca
     2581 gaagacaagg agaattacaa gaaattctat gaggcattct ctaaaaatct caagcttgga
     2641 atccacgaag actccactaa ccgccgccgc ctgtctgagc tgctgcgcta tcatacctcc
     2701 cagtctggag atgagatgac atctctgtca gagtatgttt ctcgcatgaa ggagacacag
     2761 aagtccatct attacatcac tggtgagagc aaagagcagg tggccaactc agcttttgtg
     2821 gagcgagtgc ggaaacgggg cttcgaggtg gtatatatga ccgagcccat tgacgagtac
     2881 tgtgtgcagc agctcaagga atttgatggg aagagcctgg tctcagttac caaggagggt
     2941 ctggagctgc ctgaggatga ggaggagaag aagaagatgg aagagagcaa ggcaaagttt
     3001 gagaacctct gcaagctcat gaaagaaatc ttagataaga aggttgagaa ggtgacaatc
     3061 tccaatagac ttgtgtcttc accttgctgc attgtgacca gcacctacgg ctggacagcc
     3121 aatatggagc ggatcatgaa agcccaggca cttcgggaca actccaccat gggctatatg
     3181 atggccaaaa agcacctgga gatcaaccct gaccacccca ttgtggagac gctgcggcag
     3241 aaggctgagg ccgacaagaa tgataaggca gttaaggacc tggtggtgct gctgtttgaa
     3301 accgccctgc tatcttctgg cttttccctt gaggatcccc agacccactc caaccgcatc
     3361 tatcgcatga tcaagctagg tctaggtatt gatgaagatg aagtggcagc agaggaaccc
     3421 aatgctgcag ttcctgatga gatcccccct ctcgagggcg atgaggatgc gtctcgcatg
     3481 gaagaagtcg attagtcgac ggtaccgcgg gcccgggatc caccggatct agataactga
     3541 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     3601 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     3661 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3721 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta
     3781 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     3841 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     3901 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     3961 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     4021 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     4081 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     4141 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     4201 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
     4261 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4321 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc
     4381 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     4441 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     4501 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     4561 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     4621 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     4681 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
     4741 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     4801 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     4861 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     4921 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     4981 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     5041 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     5101 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     5161 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     5221 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     5281 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     5341 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     5401 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     5461 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     5521 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     5581 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     5641 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     5701 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg
     5761 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
     5821 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     5881 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     5941 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt
     6001 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6061 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6121 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6181 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6241 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6301 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6361 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6421 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6481 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6541 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6601 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6661 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6721 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6781 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6841 gtattaccgc catgcat
//
