LOCUS       sequence-20404-e         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG415GPD-ccdB
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(28..152)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(160..176)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(160..176)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(182..445)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(265..288)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 aagcctgggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt tttacagtat
       61 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
      121 gtttctcgtt cagctttttt gtacaaactt gtgatggggg atccactagt tctagaatcc
      181 gtcgaaacta agttctggtg ttttaaaact aaaaaaaaga ctaactataa aagtagaatt
      241 taagaagttt aagaaataga tttacagaat tacaatcaat acctaccgtc tttatatact
      301 tattagtcaa gtaggggaat aatttcaggg aactggtttc aacctttttt ttcagctttt
      361 tccaaatcag agagagcaga aggtaataga aggtgtaaga aaatgagata gatacatgcg
      421 tgggtcaatt gccttgtgtc atcatttact ccaggcaggt tgcatcactc cattgaggtt
      481 gtgcccgttt tttgcctgtt tgtgcccctg ttctctgtaa ttgcgctaag agaatggacc
      541 tatgaactga aggttggtga agaaaacaat attttggtgc tgggattctt tttttttctg
      601 gatgccagct taaa
//
