LOCUS       sequence-204041-       10989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of human SIK3 cDNA (gatekeeper 
            mutation) with neomycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Tarumoto Y, Lu B, Somerville TDD, Huang YH, Milazzo JP, Wu XS, 
            Klingbeil O, El Demerdash O, Shi J, Vakoc CR
  TITLE     LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in 
            Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011.
  PUBMED    29526696
REFERENCE   2  (bases 1 to 10989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011."
COMMENT     Sequence Label: LentiV_Neo_SIK3_T142Q
FEATURES             Location/Qualifiers
     source          1..10989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             5961..6017
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6036..6830
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6090..6109)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6700..6719
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6846..7434
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6899..6919)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7317..7328)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7506..7739
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7817..7938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7854..7873)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7908..7927
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7978..8113
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8133..8152)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8134..8152)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8162..8179)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8162..8178)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8171..8193)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8320..8775
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8407..8426)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8617..8638
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8801..8905
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8906..8974,8975..9766)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9124..9143)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9937..10525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10426..10445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10679..10696
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10813..10834
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10849..10854,10855..10872,10873..10879)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10887..10903
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10892..10914
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10911..10927
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10911..10927
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10946..10966
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10948..10966
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccacccggag gtttcactgc
     2161 acaacaagat ggcggcggcg gcggcgagcg gagctggcgg ggctgccggg gccgggactg
     2221 ggggagccgg gcccgcgggc cgcctgctgc ctccgcccgc gccggggtcc ccagccgccc
     2281 ccgctgccgt gtcccctgcg gccggccagc cgcgtccccc agccccggcc tcccgcggac
     2341 ccatgcccgc ccgtatcggc tactacgaga tcgaccgcac catcggcaag ggcaacttcg
     2401 cggtggtcaa gcgggccacg cacctcgtca ccaaggccaa ggttgctatc aagatcatag
     2461 ataagaccca gctggatgaa gaaaacttga agaagatttt ccgggaagtt caaattatga
     2521 agatgctttg ccacccccat atcatcaggc tctaccaggt tatggagaca gaacggatga
     2581 tttatctggt gcaagaatat gctagtggag gggaaatatt tgaccacctg gtggcccatg
     2641 gtagaatggc agaaaaggag gcacgtcgga agttcaaaca gatcgtcaca gctgtctatt
     2701 tttgtcactg tcggaacatt gttcatcgtg atttaaaagc tgaaaattta cttctggatg
     2761 ccaatctgaa tatcaaaata gcagattttg gtttcagtaa cctcttcact cctgggcagc
     2821 tgctgaagac ctggtgtggc agccctccct atgctgcacc tgaactcttt gaaggaaaag
     2881 aatatgatgg gcccaaagtg gacatctgga gccttggagt tgtcctctac gtgcttgtgt
     2941 gcggtgccct gccatttgat ggaagcacac tgcagaatct gcgggcccgc gtgctgagtg
     3001 gaaagttccg catcccattt tttatgtcca cagaatgtga gcatttgatc cgccatatgt
     3061 tggtgttaga tcccaataag cgcctctcca tggagcagat ctgcaagcac aagtggatga
     3121 agctagggga cgccgatccc aactttgaca ggttaatagc tgaatgccaa caactaaagg
     3181 aagaaagaca ggtggacccc ctgaatgagg atgtcctctt ggccatggag gacatgggac
     3241 tggacaaaga acagacactg caggcggagc aggcaggtac tgctatgaac atcagcgttc
     3301 cccaggtgca gctgatcaac ccagagaacc aaattgtgga gccggatggg acactgaatt
     3361 tggacagtga tgagggtgaa gagccttccc ctgaagcatt ggtgcgctat ttgtcaatga
     3421 ggaggcacac agtgggtgtg gctgacccac gcacggaagt tatggaagat ctgcagaagc
     3481 tcctacctgg ctttcctgga gtcaaccccc aggctccatt cctgcaggtg gcccctaatg
     3541 tgaacttcat gcacaacctg ttgcctatgc aaaacttgca accaaccggg caacttgagt
     3601 acaaggagca gtctctccta cagccgccca cgctacagct gttgaatgga atgggccccc
     3661 ttggccggag ggcatcagat ggaggagcca acatccaact gcatgcccag cagctgctga
     3721 agcgcccacg gggaccctct ccgcttgtca ccatgacacc agcagtgcca gcagttaccc
     3781 ctgtggacga ggagagctca gacggggagc cagaccagga agctgtgcag aggtacttgg
     3841 caaataggtc caaaagacat acactggcca tgaccaaccc tacagctgag atcccaccgg
     3901 acctacaacg gcagctagga cagcagcctt tccgttcccg ggtctggcct cctcacctgg
     3961 tacctgatca gcatcgctct acctacaagg actccaacac tctgcacctc cctacggagc
     4021 gtttctcccc tgtgcgccgg ttctcagatg gggctgcgag catccaggcc ttcaaagctc
     4081 acctggaaaa aatgggcaac aacagcagca tcaaacagct gcagcaggag tgtgagcagc
     4141 tgcagaagat gtacgggggg cagattgatg aaagaaccct ggagaagacc cagcagcagc
     4201 atatgttata ccagcaggag cagcaccatc aaattctcca gcaacaaatt caagactcta
     4261 tctgtcctcc tcagccatct ccacctcttc aggctgcatg tgaaaatcag ccagccctcc
     4321 ttacccatca gctccagagg ttaaggattc agccttcaag cccacccccc aaccacccca
     4381 acaaccatct cttcaggcag cccagtaata gtcctccccc catgagcagt gccatgatcc
     4441 agcctcacgg ggctgcatct tcttcccagt ttcaaggctt accttcccgc agtgcaatct
     4501 ttcagcagca acctgagaac tgttcctctc ctcccaacgt ggcactaacc tgcttgggta
     4561 tgcagcagcc tgctcagtca cagcaggtca ccatccaagt ccaagagcct gttgacatgc
     4621 tcagcaacat gccaggcaca gctgcaggct ccagtgggcg cggcatctcc atcagcccca
     4681 gtgctggtca gatgcagatg cagcaccgta ccaacctgat ggccaccctc agctatgggc
     4741 accgtccctt gtccaagcag ctgagtgctg acagtgcaga ggctcacagt gcacatcagc
     4801 agccgccaca ctataccacg tcggcactac agcaggccct gctgtctccc acgccgccag
     4861 actatacaag acaccagcag gtaccccaca tccttcaagg actgctttct ccccggcatt
     4921 cgctcaccgg ccactcggac atccggctgc ccccaacaga gtttgcacag ctcattaaaa
     4981 ggcagcagca acaacggcag cagcagcagc aacagcagca acagcaagaa taccaggaac
     5041 tgttcaggca catgaaccaa ggggatgcgg ggagtctggc tcccagcctt gggggacaga
     5101 gcatgacaga gcgccaggct ttatcttatc aaaatgctga ctcttatcac catcacacca
     5161 gcccccagca tctgctacaa atcagggcac aagaatgtgt ctcacaggct tcctcaccca
     5221 ccccgcccca cgggtatgct caccagccgg cactgatgca ttcagagagc atggaggagg
     5281 actgctcgtg tgagggggcc aaggatggct tccaagacag taagagttca agtacattga
     5341 ccaaaggttg ccatgacagc cctctgctct tgagtaccgg tggacctggg gaccctgaat
     5401 ctttgctagg aactgtgagt catgcccaag aattggggat acatccctat ggtcatcagc
     5461 caactgctgc attcagtaaa aataaggtgc ccagcagaga gcctgtcata gggaactgca
     5521 tggatagaag ttctccagga caagcagtgg agctgccgga tcacaatggg ctcgggtacc
     5581 cagcacgccc ctccgtccat gagcaccaca ggccccgggc cctccagaga caccacacga
     5641 tccagaacag cgacgatgct tatgtacagc tggataactt gccaggaatg agtctcgtgg
     5701 ctgggaaagc acttagctct gcccggatgt cggatgcagt tctcagtcag tcttcgctca
     5761 tgggcagcca gcagtttcag gatggggaaa atgaggaatg tggggcaagc ctgggaggtc
     5821 atgagcaccc agacctgagt gatggcagcc agcatttaaa ctcctcttgc tatccatcta
     5881 cgtgtattac agacattctg ctcagctaca agcaccccga agtctccttc agcatggagc
     5941 aggcaggcgt gggcagcggc gccaccaact tcagcctgct gaagcaggcc ggcgacgtgg
     6001 aggagaaccc cggccccatg accgagtaca agcccatgat tgaacaagat ggattgcacg
     6061 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     6121 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     6181 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     6241 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     6301 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     6361 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     6421 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     6481 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     6541 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     6601 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     6661 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     6721 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     6781 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga acgcgttaag
     6841 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6901 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6961 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     7021 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     7081 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     7141 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7201 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     7261 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7321 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7381 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac
     7441 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     7501 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     7561 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7621 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7681 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7741 tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     7801 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7861 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7921 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     7981 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8041 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     8101 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     8161 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     8221 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     8281 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     8341 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     8401 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     8461 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     8521 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     8581 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     8641 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     8701 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     8761 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     8821 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     8881 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     8941 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     9001 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     9061 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     9121 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     9181 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     9241 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     9301 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     9361 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     9421 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     9481 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     9541 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     9601 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     9661 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     9721 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     9781 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     9841 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     9901 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     9961 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    10021 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10081 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    10141 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    10201 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    10261 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    10321 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    10381 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    10441 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    10501 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    10561 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    10621 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    10681 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
    10741 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
    10801 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
    10861 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
    10921 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
    10981 gctgcaagc
//
