LOCUS       sequence-20408-e         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG416GAL-ccdB
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(120..244)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(277..293)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(278..294)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      296..543
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(312..330)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(561..580)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(562..580)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(590..607)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(590..606)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(599..621)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
       61 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
      121 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
      181 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
      241 tggtgatggg ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc tcgagtcatg
      301 taattagtta tgtcacgctt acattcacgc cctcccccca catccgctct aaccgaaaag
      361 gaaggagtta gacaacctga agtctaggtc cctatttatt tttttatagt tatgttagta
      421 ttaagaacgt tatttatatt tcaaattttt cttttttttc tgtacagacg cgtgtacgca
      481 tgtaacatta tactgaaaac cttgcttgag aaggttttgg gacgctcgaa ggctttaatt
      541 tgcggccggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
      601 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      661 atcccccttt
//
