LOCUS       sequence-204080-       10932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses murine non-phosphorylatable Cep215 at S608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10932)
  AUTHORS   Colicino EG, Garrastegui AM, Freshour J, Santra P, Post DE, Kotula 
            L, Hehnly H
  TITLE     Gravin regulates centrosome function through PLK1.
  JOURNAL   Mol Biol Cell. 2017 Dec 27. pii: mbc.E17-08-0524. doi: 
            10.1091/mbc.E17-08-0524.
  PUBMED    29282278
REFERENCE   2  (bases 1 to 10932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2017 Dec 27. pii: mbc.E17-08-0524. doi: 
            10.1091/mbc.E17-08-0524."
COMMENT     Sequence Label: (PD-S608A)FLAG-Cep215-FL
FEATURES             Location/Qualifiers
     source          1..10932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..59)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        231..610
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        611..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     765..785
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     859..878
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        859..877
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      891..900
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             900..971
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     promoter        complement(6481..6499)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6482..6499)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6519..6536)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6525..6749
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6795..7223
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6882..6901)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7092..7113
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7232..7252)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7237..7566
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7417..7552
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7479..7498
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7633..8427
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7687..7706)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8297..8316
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8601..8722
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8638..8657)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8692..8711
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8771..8787)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8771..8787)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8784..8806)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8795..8811
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8819..8825,8826..8843,8844..8849))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8864..8885
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9002..9019)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9173..9761)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9253..9272)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9932..10723,10724..10792))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10555..10574
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10793..10897)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
       61 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
      121 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
      181 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
      241 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
      301 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      361 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      421 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      481 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      541 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      601 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      661 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      721 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      781 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc
      841 ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt gccaccatgg
      901 attacaagga tgacgacgat aaggattaca aggatgacga cgataaggat tacaaggatg
      961 acgacgataa gggatctatg gactcgggga tggaagagga aggcgccctg cctgggaccc
     1021 tcagcggttg cagtggcctt caccctgttc tgccaagtga cctggatgtc atcagtgaca
     1081 cttctggctt gggaaatgga gtgctcccaa gcatgtcaga agaaaaggtt tctcccacca
     1141 gagcccggaa catgaaggac tttgaaaacc aaatcactga gctgaagaag gagaacttca
     1201 atctaaagct ccggatctac ttcctggagg agagaatcca gcaggagttt gctggcccca
     1261 ccgagcacat ctacaagacg aacattgaac tcaaggtgga agtggaaagt ctgaagcgcg
     1321 agctccagga gaaagatcag ttgctcgtca aagcctccaa agcggtggag agcctagcag
     1381 aacggggtgg ttctgaagtc cagcgggtga aagaagatgc aaggaagaag gtgcagcagg
     1441 tggaagacct cctaacgaaa aggatacatc ttctagaaga agatgtgaaa gccgcccagg
     1501 cagaactgga gaaggccttt gcggggacag aaacagagaa ggctcttcgg ctcagcttag
     1561 aaagcaagct ctcggcgatg aagaagatgc aggaggggga cctggagatg actctggctc
     1621 tggaagagaa ggacagactg attgaggagt tgaagctgtc tttgaagagc aaagaagctt
     1681 taattcagtg ccttaaagag gaaaaatctc agatggcatc tcctgacgag aacgtgtcat
     1741 ctggagaact ccgaggactt tctgccactc tgagggaaga aaaagagaga gacgctgagg
     1801 aatggcagaa ggagagaaac cactttgaag agaggatcca ggcacttcag gaggacctga
     1861 gagagaagga aagagaaatt gctacagaga agaaaaataa cttaaagagt tataaagcca
     1921 ttcagggtct aaccatggca ttaaaatcaa aggaaagaga ggttgaagaa cttgattcca
     1981 aaatcaaaga ggtcactacg gactccacca aaggcagaga ggatccctta aaaacccaga
     2041 tccccagatt ccagctaaga gaggggtctg aggactgtga agctgcgctg gtagagaagg
     2101 aagccctatt ggccaagcta cactcagaaa atgtcacaaa gagcactgag aaccataggt
     2161 tgctcaggaa tgttaaaaag gtcacccagg agttgaatga cttgaagaag gagaagttga
     2221 gactagagca ggccctggag gaagcccacc aagaggggaa cagaggagcc cgaaccattc
     2281 acgaccttag aaatgaagtt gagaaactac gcaaggaagt gagtgagaga gagaaggctg
     2341 tggaaaaaca ttacaagagt cttccaggtg aaagcaagac aaagtttcat actcaagaac
     2401 aagtggtcag aagtctgaca ggcagtggca gtcaggagga cctgctgctt cagaaatcca
     2461 atgaaaaaga tttggaagca atacagcaga actgctattt aatgactgca gaggagctca
     2521 agtttgggag cgatggctta ataacagaaa agtgttctca gcagtcacca gacagcaaac
     2581 tcatcttctc caaggaaaag caacaatcag agtatgaagg gctgactggg gacttaaaag
     2641 cagaacagaa catctatgcc catctggcaa agactcagga cacagacagt gtcagcaacc
     2701 tgcaggctga gctgaaagag gtccttgccc ttcggaagca gctggagcag gatgttcttg
     2761 cctaccggaa cctgcagaag gccctgcagg agcagctcgc tgagattcgg agtcgagaag
     2821 aagagccgtt ttcattttat agcgatcaaa catcgtatct gagtatttgc cttgaagagc
     2881 acaatcagtt ccaattggag catttttctc aagaagaact caagaaaaag gtcagtgacc
     2941 tcatacagct tgtgaaggac ctgcacacag acaaccagca cctgaagaaa accatttttg
     3001 atctctcttc tgtgggtttc cagggaagtg acagacttga gttaacaaaa caagaagagc
     3061 ttgtggctag caaggaggat gaggacacac tcaaatttga agcggacgtt gagactcctt
     3121 tccagagtga ccagcatttg gagcaaagtc gtgagatcat ggaggactat gctgagggtg
     3181 gctgcaaaag tggatatgga aggcacatgg attccaatat tttaggtcat gatggggccc
     3241 aaactcctgg tgcctctgaa gagcacaccc tagaagatga gctcctgggc ctgcttgcca
     3301 ctctcttcag taagaaggcc acaccgttgc tggaatccag gccagatctt ctgaaagcac
     3361 tcggtgccct acttttggaa cgcatatgct tggcagagca gggtcgtcct ggagatcacc
     3421 ttgatagtaa aactgagaag gcacttcagc aagtagctgt tcagttgaga gatgaacttg
     3481 gccattcctt ccctgccaac tcgttctcca agtcatacaa tgaagtcaag tcaatgtggg
     3541 gaaattggct ggtaaagaca ggagacgaag acacggtgga gctgaagagt gtttcagtgc
     3601 agaccatggc catagaggac accccgcatg gattcaaacc tcaaagcaag agagatgctt
     3661 gggcagagaa acaagaagag gccattttca gcactgagct tgagtcagag gcgttgggtg
     3721 agatgccagg gcaacaggcc actcacctct cattccccag tgccatcaac ccggatgccg
     3781 agaagacggg cttgttgatc caattaaaga cacctgagtt attggaaaat ttatataatc
     3841 ttcctgcttc gccagaggtg gtggtggctc aactccaagg ccaggttcta gagctgcaga
     3901 gggagctgaa agaattcaaa acccgcaaca agcaactcca tgagaagcta attctggcag
     3961 aggcaatgat ggaggggctg ccggtgccca actctgcgct ggtgaatgtg cctgcagccc
     4021 aggctgttgt gagaacagcc ttccaggaca acccaggcga gcaagaagga cctgaaacca
     4081 cacagtctgc tgggagagac aaggacatgg acagtgacca atatacaagc tttgagatcg
     4141 attctgaaat ctgtccacct gatgaccttg ccttattacc agcatgcaaa gagaatcttg
     4201 aagacctcct aggcccatcc tccatagcca cttacctgga ttccaagagt cagctctctg
     4261 ttaaagtcag cgtgaatgga actgaccagt ctgagaatat caatatccct gatgacacag
     4321 aagacttaaa acagaaaatc cacgacttgc aaaccgagct ggaaggctac cggaacatca
     4381 ttgtccagct actgaagcac tctcagtgca gtgaggctat tatcacagtt ttgtgtggga
     4441 cagaaggggc ccaagatggc ttgaacaagc ctaagggcca tattgatgag gaagagatga
     4501 cattttcaag tttgcaccaa gtacgatatg tgaagcatat gaaaatcctc cgtccactaa
     4561 ccccggagat gattgatggc aagatgctag agagcctcaa gcagcagctg gtggaccagg
     4621 agcaggagct acagaaggag caggacttga acttggaact tttcggtgaa atccatgacc
     4681 tgcagaacaa gttccaagac ctctcgccat caagatacga ttcattggtt cagtcccaag
     4741 cgagggagct ctcccttcag cgacagcaaa ttaaggatag ccatggcatc tgtgtcattt
     4801 accgtcaaca catgagcacc atgatcaagg cttttgagga gcttctgcag gccagtgatg
     4861 tggacagttg cgtggccgag ggcttccggg agcagctgac acagtgtgct ggactgctag
     4921 agcaactgga gaggctgttt ctccatggaa aatcagccag agtggaacca catccccaga
     4981 atgaactgct caaggggtta aggactgtgg aaggcaactt accctaccac catctcctgc
     5041 ctgaatctcc tgagccttct gcctcccatg ctctgtcaga tgatgaaatg tctgagaagt
     5101 ccttcctgtc tagagacccg aagccagaca gcgatactga gaagtaccca gcaatggcga
     5161 gccattttcc tcaagactta ctaatggaac acatacagga aattcgaact ttgagaaagc
     5221 atttggaaga atctattaaa acaaatgaga agctccggaa acagctggaa cggcaggggt
     5281 ctgagactga ccaaggctcc agaaatgttt ctgcttgtgg cttagcactg cacagctctc
     5341 tgacgtcaga gatacatttc ctgaggaagc agaacgaggc cctcagtatg atgctcgaga
     5401 aggggtccaa agataaacag aaagagagtg aaaaattacg tgagtccctg gctaggaaag
     5461 ctgagagtct tgagcaactt cagctagagt acaccagtgt aagggaagaa aatgaaaggt
     5521 tacagagaga catcattgag aaggagagac acaaccagga gctgactgag gaggtctgca
     5581 gcagccgcca ggaactgagc agggtgcagg aagaggcgaa gtcaagacag cagctgctct
     5641 cacagaagga caagctgctt cagtccctcc aaatggagct caaggtgtat gagaaactgg
     5701 ccgaggaaca tccgaggcta cagcaagatg gcagcaaatg tcctgaggcc agcgacaatt
     5761 catttgatct atttgaatcc acccaggcaa tggcacctaa atcagcttct gagactcctc
     5821 tgctctcagg aactgatgtt gactcgctct cctgtgacag taccagttct gccaccagcc
     5881 ctactagcat gccctgcctg gttgctggcc accacatgtg ggccagcaag agtggccacc
     5941 atatgctggg cctgattgag gactatgatg ccctctataa gcagatcagc tggggccaga
     6001 cactgcttgc caagatggat gttcaaaccc aagaggctct gagccccaca agtcacaagc
     6061 tgggaccaaa gggttcttca tccgtgcctc tgagcaagtt tctttccagt atgaacacag
     6121 ccaagctggt cctggaaaaa gcatccaggt tgctgaagct cttctggagg gtctcagtcc
     6181 ccaccaacgg ccagtgttcc cttcactgtg agcagattgg agaaatgaag gcagagaaca
     6241 cgaaactaca caaaaaattg tttgaacaag aaaagaagct gcagaacaca gcaaaactat
     6301 tgcagcagag caagcaccag gagaaagtca tctttgatca gttggtcatc acccaccaag
     6361 tccttcggaa ggccagggga aacctggagc tcaggcctgg ggccacccgt ccaggagcat
     6421 ccagtcccag cagaccgggc tcatgagcgg ccgctcgagc atgcatctag agggccctat
     6481 tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
     6541 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     6601 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     6661 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     6721 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     6781 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     6841 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     6901 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     6961 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     7021 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7081 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     7141 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     7201 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     7261 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7321 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7381 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7441 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7501 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     7561 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     7621 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     7681 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     7741 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     7801 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     7861 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     7921 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     7981 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     8041 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     8101 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     8161 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     8221 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     8281 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     8341 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     8401 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     8461 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     8521 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     8581 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     8641 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8701 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     8761 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     8821 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     8881 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     8941 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     9001 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     9061 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     9121 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     9181 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     9241 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     9301 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     9361 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     9421 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     9481 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     9541 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     9601 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9661 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     9721 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9781 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9841 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9901 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9961 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    10021 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    10081 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    10141 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    10201 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    10261 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    10321 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    10381 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    10441 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    10501 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    10561 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    10621 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    10681 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    10741 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10801 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10861 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    10921 ctgacgtcga cg
//
