LOCUS       sequence-204094-        9048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dox-inducible mir30 shREN (control shRNA)/dsRED expression with 
            Venus marker and Neo resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Xu Y, Milazzo JP, Somerville TDD, Tarumoto Y, Huang YH, Ostrander 
            EL, Wilkinson JE, Challen GA, Vakoc CR
  TITLE     A TFIID-SAGA Perturbation that Targets MYB and Suppresses Acute 
            Myeloid Leukemia.
  JOURNAL   Cancer Cell. 2018 Jan 8;33(1):13-28.e8. doi: 
            10.1016/j.ccell.2017.12.002.
  PUBMED    29316427
REFERENCE   2  (bases 1 to 9048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2017.12"; journalName: "Cancer Cell"; date: 
            "2018-01-8- 8"; volume: "33"; issue: "1"; pages: "13-28.e8"
COMMENT     Sequence Label: TRIN-shREN
FEATURES             Location/Qualifiers
     source          1..9048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..463
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(57..74)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     284..303
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             479..1273
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             479..1273
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(533..552)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1143..1162
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             1412..1837
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(1940..1962)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2098..2118)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2103..2432
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2283..2418
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2454..2473
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2554..2571)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2725..3313)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2805..2824)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3484..4275,4276..4344))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4107..4126
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4345..4449)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4517..4535
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        4635..5014
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5015..5218
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             5219..5394
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    5457..5656
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             5857..6273
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     6203..6225
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6241..6257
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    6323..6341
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6365..6383
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6407..6425
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6449..6467
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6491..6509
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6533..6551
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        6584..6622
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6619..6643
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6721..7398
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(6901..6921)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     7313..7336
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     ncRNA           7467..7588
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           7616..7630
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           7658..7784
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        7807..8306
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7842..7865)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8239..8258
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(8329..8331,8332..8334,8335..9048)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8374..8395)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8635..8654)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8982..9003
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
       61 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
      121 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
      181 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
      241 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
      301 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
      361 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
      421 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttacc ggtccaccat
      481 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
      541 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
      601 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
      661 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
      721 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
      781 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
      841 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
      901 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
      961 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     1021 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     1081 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     1141 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     1201 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     1261 cgagttcttc tgatgtacga tgcatcgata ccgtcgagat atcagtggtc caggctctag
     1321 ttttgactca acaatatcac cagctgaagc ctatagagta cgagccatag ataaaataaa
     1381 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
     1441 agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga
     1501 agttcagatc aaggtcagga acagatggaa cagggtcgac cctagagaac catcagatgt
     1561 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
     1621 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
     1681 ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa
     1741 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc
     1801 tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc gggatcggga
     1861 gacccctgcc cagggaccac cgacccacca ccgggaggta agctggctgc ctcgcgcgtt
     1921 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     1981 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     2041 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtagatc cggctgtgga
     2101 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     2161 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     2221 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     2281 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     2341 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     2401 gaggcttttt tggaggccta ggcttttgca aaaagcttac tggcttaact atgcggcatc
     2461 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     2521 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2581 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2641 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2701 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2761 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2821 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2881 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2941 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3001 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3061 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3121 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     3181 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3241 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3301 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3361 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3421 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3481 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3541 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3601 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     3661 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     3721 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     3781 caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     3841 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     3901 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     3961 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4021 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4081 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
     4141 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4201 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4261 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4321 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4381 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4441 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     4501 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattagcttg
     4561 gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct catgtccaac
     4621 attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     4681 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     4741 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     4801 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     4861 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     4921 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     4981 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     5041 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     5101 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     5161 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctca
     5221 ataaaagagc ccacaacccc tcactcggcg cgccagtctt ccgatagact gcgtcgcccg
     5281 ggtacccgta ttcccaataa agcctcttgc tgtttgcatc cgaatcgtgg tctcgctgtt
     5341 ccttgggagg gtctcctctg agtgattgac tacccacgac gggggtcttt catttggggg
     5401 ctcgtccggg atttggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
     5461 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgtttga tgttatgcgc
     5521 ctgcgtctgt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
     5581 gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt
     5641 tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt
     5701 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
     5761 tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc
     5821 tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta
     5881 agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat
     5941 gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga
     6001 tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt
     6061 tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg
     6121 gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt
     6181 cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc
     6241 ctttatccag ccctcactcc ttctctaggc gccggaattg aagatctggg ggatcgatcc
     6301 tctagagcat cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     6361 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     6421 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     6481 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     6541 agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg
     6601 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
     6661 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctctcga cggatccacc
     6721 atggcctcct ccgagaacgt catcaccgag ttcatgcgct tcaaggtgcg catggagggc
     6781 accgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     6841 cacaacaccg tgaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
     6901 ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc cgacatcccc
     6961 gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     7021 gacggcggcg tggcgaccgt gacccaggac tcctccctgc aggacggctg cttcatctac
     7081 aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtgatgca gaagaagacc
     7141 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
     7201 acccacaagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagtccatc
     7261 tacatggcca agaagcccgt gcagctgccc ggctactact acgtggacgc caagctggac
     7321 atcacctccc acaacgagga ctacaccatc gtggagcagt acgagcgcac cgagggccgc
     7381 caccacctgt tcctgtagcg gccgcgactc tagaatttac tagtcatatg ataagatctc
     7441 tcgactaggg ataacagggt aattgtttga atgaggcttc agtactttac agaatcgttg
     7501 cctgcacatc ttggaaacac ttgctgggat tacttcttca ggttaaccca acagaaggct
     7561 cgagaaggta tattgctgtt gacagtgagc gcaggaatta taatgcttat ctatagtgaa
     7621 gccacagatg tatagataag cattataatt cctatgccta ctgcctcgga attcaagggg
     7681 ctactttagg agcaattatc ttgtttacta aaactgaata ccttgctatc tctttgatac
     7741 atttttacaa agctgaatta aaatggtata aattaaatca cttttttcaa ttctagcaat
     7801 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     7861 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     7921 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     7981 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     8041 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     8101 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     8161 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     8221 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     8281 cctcttcctc atctccgggc ctttcgacct gcagcccaag cttccaccat ggtgagcaag
     8341 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     8401 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     8461 ctgaagctga tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     8521 ctgggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     8581 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     8641 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     8701 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     8761 aactacaaca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     8821 aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag
     8881 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac
     8941 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     9001 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaa
//
