LOCUS       sequence-204096-       10154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  full-length murine SSeCKs/AKAP12/Gravin in retroviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10154)
  AUTHORS   Colicino EG, Garrastegui AM, Freshour J, Santra P, Post DE, Kotula 
            L, Hehnly H
  TITLE     Gravin regulates centrosome function through PLK1.
  JOURNAL   Mol Biol Cell. 2017 Dec 27. pii: mbc.E17-08-0524. doi: 
            10.1091/mbc.E17-08-0524.
  PUBMED    29282278
REFERENCE   2  (bases 1 to 10154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2017 Dec 27. pii: mbc.E17-08-0524. doi: 
            10.1091/mbc.E17-08-0524."
COMMENT     Sequence Label: pBABE-SSeCKs
FEATURES             Location/Qualifiers
     source          1..10154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5078..5098)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5083..5412
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5263..5398
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5422..6021
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5422..5441)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5918..5938
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6242..6711
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6841..6858)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7012..7600)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7092..7111)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7760..8551,8552..8620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8383..8402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8621..8725)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             8756..9225
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    9290..9489
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             9690..10106
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     10036..10058
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     10074..10090
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 ggcagttcca ccgagcagcg gagccccgag cagccggcgg agagcgacac gccgagcgag
       61 ctggagctca gtggccatgg gcccgcagcg gaagcgtcgg gagcagctgg agatcccgct
      121 gacgcggacc ccgccaccaa gctcccacag aagaatggtc agctgtctgc cgtcaatggt
      181 gtagctgaac aagaagatgt ccacgtccaa gaggaaagcc aggatgggca agaggaagaa
      241 gtcactgttg aagatgttgg acagagagag tcagaagatg tgaaagaaaa agaccgagct
      301 aaagaaatgg cagccagttc cacagttgtt gaagatatca caaaggacga gcaggaggaa
      361 acaccggaaa taatcgaaca gatccctgct tcagagagca atgtggaaga aatggcgcag
      421 gctgctgagt cccaagctaa tgacgtcggc ttcaagaagg tatttaaatt tgttggtttt
      481 aaattcacgg tgaagaagga taaaaacgaa aagtcagata ccgtccagct actcactgtc
      541 aagaaggatg aaggcgaagg ggcagaagcc tccgtcggag caggagacca ccaagagccc
      601 ggagtggaga ccgtcggcga atcagcatcc aaagaaagtg agctgaagca atccacagag
      661 aagcaagaag gcaccctgaa gcaagcacag agcagcacag aaattcccct tcaagccgaa
      721 tctggtcaag ggaccgagga agaagcagcc aaagatggag aagaaaaccg agagaaagaa
      781 cctaccaagc ccctagaatc tccgaccagc cctgtcagca atgagacaac atcttccttc
      841 aagaaattct tcactcacgg ctgggccggc tggcgcaaga agaccagctt caagaaacca
      901 aaggaagatg atctggaaac ttccgagaag agaaaggagc aagaggctga aaaagtagac
      961 gaggaagaag gggaaaagac agagccagcc ccagccgagg agcaggagcc tgcagaaggc
     1021 acagaccagg ccaggttgtc agccgactat gagaaggtgg agttgccttt ggaagaccag
     1081 gtcggtgacc tggaggcatt gtcggagaag tgtgctcctt tggcaacgga agtgtttgat
     1141 gagaagacgg aagcccacca agaagttgtt gcagaggtcc acgtgagcac cgtggagaag
     1201 atgacgaaag ggcaaggagg agcagaggtg gaaggggatg tggtggtgga aggatcggga
     1261 gaatccttgc cccctgagaa actggctgag acccaggagg tcccccagga agctgagcct
     1321 gtggaggagc tgatgaagac caaagaagta tgcgtctctg ggggtgacca tactcagctg
     1381 acagatctaa gtcctgaaga gaagatgcta cccaaacacc ccgaaggcat tgtcagtgag
     1441 gtggagatgc tgtcctctca ggagagaatc aaggtacagg gaagtcccct gaagaagctc
     1501 ttcagcagtt cgggcttaaa gaagctctcc gggaagaagc agaaggggaa gagaggagga
     1561 ggcgggggag atgaagagcc aggagaatac caacacattc aaaccgagtc cccagagagt
     1621 gctgacgagc agaagggaga gagctctgcc tcttcccctg aagagcccga ggagatcgcg
     1681 tgtctggaga aggggccatc ggaagcaccc caggaagcgg aagctgagga aggagcgact
     1741 tccgacggag agaagaaaag ggaagggatc accccctggg catccttcaa aaagatggtg
     1801 acacccaaga aacgggtccg aagaccttct gagagcgaca aggaagaaga gctggataag
     1861 gtcaagagtg ccaccttgtc ctccacggag agcacggcgt ctggaatgca ggatgaggtc
     1921 agagcggttg gcgaggagca aaggtcagag gagccaaagc gcagggtgga tacttcagtg
     1981 tcttgggagg cgttgatttg tgtcggatcg tccaagaaga gagcgaggaa ggcatcctct
     2041 tcagatgatg aaggagggcc aagaacactg ggaggggatg gccacagagc ggaggaggct
     2101 agcaaagaca aagaagcaga tgctcttcct gccagcaccc aggaacaaga ccaagcgcac
     2161 ggaagttcct cacccgagcc agctggaagc ccttctgaag gggagggcgt ctccacctgg
     2221 gagtcattta agagattagt cactccacga aaaaaatcca agtcaaaact ggaagagaga
     2281 gccgaagact ccggtgcaga gcagttggcc tccgagatcg aaccaagtag agaggaatct
     2341 tgggtttcca ttaagaaatt tattcctgga cggcggaaga aaagggcaga tgggaagcaa
     2401 gaacaggccg ccgttgaaga ctcggggcca ggagagatca atgaggacga ccccgacgtc
     2461 ccagctgttg tgcctctgtc tgagtacgat gcggtagaga gagagaagct ggaagcgcag
     2521 cgagctcagg agaacgtgga gctgccccag ctgaaggggg ctgtgtatgt gtctgaggag
     2581 cttagtaaga ctctggttca caccgtgagt gtcgcggtca ttgatgggac cagggcagtc
     2641 accagtgccg aagagcggtc cccttcgtgg atatctgctt ccatgacaga acctcttgag
     2701 cacgcagagg gagtggccac accgcctgtt ggagaggtca ctgaaaaaga catcactgca
     2761 gaagcaactc ctgcactcgc ccagacttta ccagggggca aagatgccca tgacgacata
     2821 gtcaccagtg aagtggattt tacctcagaa gcagtgacag ccgcagaaac cacagaggcg
     2881 ctccgcgctg aagaacttac cgaagcatca ggggcagaag agaccacaga catggtgtct
     2941 gcagtttccc agctgtccga ctccccggac accacagagg aagccacccc agttcaggag
     3001 gtagagggtg gcatgctaga tacggaagaa caggagcgcc agacgcaggc cgtcctccaa
     3061 gccgttgcag acaaagtgaa agaggactcc caggtgcctg caacccagac tctgcagaga
     3121 gcagggccga aagcactgga gaaggtggag gaggtagagg aggactccga ggtgctggct
     3181 accgagaaag agaaggatgt tgtgccggaa ggacccgtgc aggaagctga aactgagcat
     3241 cttgcacagg gctccgagac tgtacaggct accccagaga gccttgaagt tcctgaagtc
     3301 acagaggatg tagaccgtgc caccacatgc caggttatca agcaccagca gctgatggaa
     3361 caggctgtgg cccctgagtc atctgaaacc ttgacagaca gtgagacaaa tggaagtact
     3421 cccctcgcag attcagacac tccaaacggg acacagcaag acgagaccgt tgacagccag
     3481 gacagtaatg ccattgccgc cgtcaagcag tcacaggtca ctgaagagga ggcagctgct
     3541 gctcagacgg aggggccttc aacaccatct agttttccag cccaggaaga acacagggaa
     3601 aaaccaggaa gggatgttct agaacccaca caagcgctgg ctgccggggc agtgcctatt
     3661 ctggcaaagg ctgaggtggg tcaagagggt gaggctggcc agtttgatgg agaaaaagtc
     3721 aaagacggac agtgtgttaa agaactggag gtgcctgtgc acactggacc caacagtcaa
     3781 aagactgctg acttgacacg tgacagtgaa gtaatggaag tggccagatg tcaggaaact
     3841 gagagtaatg aagaacagag tattagcccg gagaaaagag agatgggaac cgacgttgaa
     3901 aaggaggaaa cagagaccaa gacagagcaa gccagtgaag aacatgagca ggaaacagct
     3961 gctcctgagc atgaaggaac ccaccctaag ccagtcctga cagctgacat gcctcactca
     4021 gagaggggaa aggcactggg cagccttgaa ggaagccctt ctctcccaga ccaagacaaa
     4081 gcagattgca tagaggttca agttcaaagc tcagacacac cagtcactca aacaaccgaa
     4141 gctgtgaaaa aggtcgaaga aactgtggca acttcagaga tggatgaaag tttggagtgt
     4201 gcaggtgcgc aatcattacc agctgagaag ctctccgaaa ccggtggcta cgggactctt
     4261 cagcatggag aggacaccgt gccccagggg cctgagtctc aggcagagtc catcccaata
     4321 atagtaactc ctgctcctga aagcatccta cattctgacc ttcaaagaga agtgagcgca
     4381 tcccagaaac agagatcaga tgaagataac aagccagatg ctggtcctga tgctgccagc
     4441 aaggagagtg cagcaagaga gaaaatcctc agggctgaac ctgagatctt ggaacttgag
     4501 agtaagagca ataagattgt ccagagtgtc atccagacag ccgtcgacca gtttgcacgt
     4561 acagaaacag cccccgaaac ccacgcttct gatttacaga atcaggttcc tgtgatgcag
     4621 gctgacagcc agggagcaca acagatgctg gacaaagatg aaagcgacct tcaagtctcc
     4681 ccccaagatg gaacactcag tgccgtagcc caggaaggac ttgcggtttc tgatagttct
     4741 gaaggcatga gcaaggcttc agaaatgatc accacgcttg cagttgaaag tgccagtgtc
     4801 aaagaaagtg ttgagaagct gcctcttcag tgcaaagatg aaaaggaaca tgctgctgac
     4861 ggcccccagc accaaagctt agccaaggca gaggcggatg cctctggaaa tctaaccaaa
     4921 gagtccccag acaccaacgg accaaagcta accgaggaag gagatgccct gaaagaagaa
     4981 atgaacaagg cccagacaga agaggacgac ctacaggagc caaagggaga cctgacagaa
     5041 tcctaactcg agtcgccagc acagtggtcg accctgtgga atgtgtgtca gttagggtgt
     5101 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5161 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5221 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     5281 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     5341 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5401 ggcttttgca aaaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     5461 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     5521 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     5581 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     5641 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     5701 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     5761 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     5821 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     5881 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     5941 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     6001 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
     6061 gcacgacccc atgcatcgat aaaataaaag attttattta gtctccagaa aaagggggga
     6121 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca
     6181 tggaaaaata cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca
     6241 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     6301 agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     6361 cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta
     6421 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
     6481 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
     6541 taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg
     6601 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
     6661 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc acatgcagca
     6721 tgtatcaaaa ttaatttggt tttttttctt aagtatttac attaaatggc catagttgca
     6781 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6841 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6901 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6961 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7021 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7081 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7141 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7201 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7261 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7321 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7381 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7441 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7501 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7561 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7621 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7681 atcaaaaagg atcttcacct agatcctttt gcggccgcaa atcaatctaa agtatatatg
     7741 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7801 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7861 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7921 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7981 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8041 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8101 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8161 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8221 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8281 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8341 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8401 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8461 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8521 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8581 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8641 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8701 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gcagcctgaa
     8761 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     8821 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     8881 tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac
     8941 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     9001 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     9061 agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc
     9121 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
     9181 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc
     9241 gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggca agctggccag
     9301 caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg cgcctgcgtc
     9361 ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttct
     9421 gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt ttttgtggcc
     9481 cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg gttctggtag
     9541 gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttggaacc
     9601 gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt ctctgtctga
     9661 ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc ttaagtttga
     9721 ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga
     9781 agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc
     9841 gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg
     9901 gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg
     9961 acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat
    10021 ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc
    10081 cagccctcac tccttctcta ggcgccggcc ggatcccagt gtggtggtac gtaggaatga
    10141 attcaatggg tgca
//
