LOCUS       sequence-204098-       11431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11431)
  AUTHORS   Osakada F, Mori T, Cetin AH, Marshel JH, Virgen B, Callaway EM
  TITLE     New rabies virus variants for monitoring and manipulating activity 
            and gene expression in defined neural circuits.
  JOURNAL   Neuron. 2011 Aug 25;71(4):617-31.
  PUBMED    21867879
REFERENCE   2  (bases 1 to 11431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2011-08-25"; volume: "71(4)"; pages: "617-31"
COMMENT     Sequence Label: pcDNA-SADB19L
FEATURES             Location/Qualifiers
     source          1..11431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2367..2378
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7205..7222)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7211..7435
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7481..7909
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7568..7587)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7778..7799
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7918..7938)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7923..8252
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8103..8238
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8165..8184
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8300..8347
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8366..8740
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8870..8991
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8907..8926)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8961..8980
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9040..9056)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9040..9056)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9053..9075)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9064..9080
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9088..9094,9095..9112,9113..9118))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9133..9154
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9271..9288)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9442..10030)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9522..9541)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10201..10992,10993..11061))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10824..10843
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11062..11166)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11241..11260)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtacctg gcaacacttc tcaacctgag acttacttca
      721 agatgctcga tcctggagag gtctatgatg accctattga cccaatcgag ttagaggctg
      781 aacccagagg aacccccatt gtccccaaca tcttgaggaa ctctgactac aatctcaact
      841 ctcctttgat agaagatcct gctagactaa tgttagaatg gttaaaaaca gggaatagac
      901 cttatcggat gactctaaca gacaattgct ccaggtcttt cagagttttg aaagattatt
      961 tcaagaaggt agatttgggt tctctcaagg tgggcggaat ggctgcacag tcaatgattt
     1021 ctctctggtt atatggtgcc cactctgaat ccaacaggag ccggagatgt ataacagact
     1081 tggcccattt ctattccaag tcgtccccca tagagaagct gttgaatctc acgctaggaa
     1141 atagagggct gagaatcccc ccagagggag tgttaagttg ccttgagagg gttgattatg
     1201 ataatgcatt tggaaggtat cttgccaaca cgtattcctc ttacttgttc ttccatgtaa
     1261 tcaccttata catgaacgcc ctagactggg atgaagaaaa gaccatccta gcattatgga
     1321 aagatttaac ctcagtggac atcgggaagg acttggtaaa gttcaaagac caaatatggg
     1381 gactgctgat cgtgacaaag gactttgttt actcccaaag ttccaattgt ctttttgaca
     1441 gaaactacac acttatgcta aaagatcttt tcttgtctcg cttcaactcc ttaatggtct
     1501 tgctctctcc cccagagccc cgatactcag atgacttgat atctcaacta tgccagctgt
     1561 acattgctgg ggatcaagtc ttgtctatgt gtggaaactc cggctatgaa gtcatcaaaa
     1621 tattggagcc atatgtcgtg aatagtttag tccagagagc agaaaagttt aggcctctca
     1681 ttcattcctt gggagacttt cctgtattta taaaagacaa ggtaagtcaa cttgaagaga
     1741 cgttcggtcc ctgtgcaaga aggttcttta gggctctgga tcaattcgac aacatacatg
     1801 acttggtttt tgtgtttggc tgttacaggc attgggggca cccatatata gattatcgaa
     1861 agggtctgtc aaaactatat gatcaggttc accttaaaaa aatgatagat aagtcctacc
     1921 aggagtgctt agcaagcgac ctagccagga ggatccttag atggggtttt gataagtact
     1981 ccaagtggta tctggattca agattcctag cccgagacca ccccttgact ccttatatca
     2041 aaacccaaac atggccaccc aaacatattg tagacttggt gggggataca tggcacaagc
     2101 tcccgatcac gcagatcttt gagattcctg aatcaatgga tccgtcagaa atattggatg
     2161 acaaatcaca ttctttcacc agaacgagac tagcttcttg gctgtcagaa aaccgagggg
     2221 ggcctgttcc tagcgaaaaa gttattatca cggccctgtc taagccgcct gtcaatcccc
     2281 gagagtttct gaggtctata gacctcggag gattgccaga tgaagacttg ataattggcc
     2341 tcaagccaaa ggaacgggaa ttgaagattg aaggtcgatt ctttgctcta atgtcatgga
     2401 atctaagatt gtattttgtc atcactgaaa aactcttggc caactacatc ttgccacttt
     2461 ttgacgcgct gactatgaca gacaacctga acaaggtgtt taaaaagctg atcgacaggg
     2521 tcaccgggca agggcttttg gactattcaa gggtcacata tgcatttcac ctggactatg
     2581 aaaagtggaa caaccatcaa agattagagt caacagagga tgtattttct gtcctagatc
     2641 aagtgtttgg attgaagaga gtgttttcta gaacacacga gttttttcaa aaggcctgga
     2701 tctattattc agacagatca gacctcatcg ggttacggga ggatcaaata tactgcttag
     2761 atgcgtccaa cggcccaacc tgttggaatg gccaggatgg cgggctagaa ggcttacggc
     2821 agaagggctg gagtctagtc agcttattga tgatagatag agaatctcaa atcaggaaca
     2881 caagaaccaa aatactagct caaggagaca accaggtttt atgtccgaca tacatgttgt
     2941 cgccagggct atctcaagag gggctcctct atgaattgga gagaatatca aggaatgcac
     3001 tttcgatata cagagccgtc gaggaagggg catctaagct agggctgatc atcaagaaag
     3061 aagagaccat gtgtagttat gacttcctca tctatggaaa aacccctttg tttagaggta
     3121 acatattggt gcctgagtcc aaaagatggg ccagagtctc ttgcgtctct aatgaccaaa
     3181 tagtcaacct cgccaatata atgtcgacag tgtccaccaa tgcgctaaca gtggcacaac
     3241 actctcaatc tttgatcaaa ccgatgaggg attttctgct catgtcagta caggcagtct
     3301 ttcactacct gctatttagc ccaatcttaa agggaagagt ttacaagatt ctgagcgctg
     3361 aaggggagag ctttctccta gccatgtcaa ggataatcta tctagatcct tctttgggag
     3421 ggatatctgg aatgtccctc ggaagattcc atatacgaca gttctcagac cctgtctctg
     3481 aagggttatc cttctggaga gagatctggt taagctccca agagtcctgg attcacgcgt
     3541 tgtgtcaaga ggctggaaac ccagatcttg gagagagaac actcgagagc ttcactcgcc
     3601 ttctagaaga tccgaccacc ttaaatatca gaggaggggc cagtcctacc attctactca
     3661 aggatgcaat cagaaaggct ttatatgacg aggtggacaa ggtggaaaat tcagagtttc
     3721 gagaggcaat cctgttgtcc aagacccata gagataattt tatactcttc ttaatatctg
     3781 ttgagcctct gtttcctcga tttctcagtg agctattcag ttcgtctttt ttgggaatcc
     3841 ccgagtcaat cattggattg atacaaaact cccgaacgat aagaaggcag tttagaaaga
     3901 gtctctcaaa aactttagaa gaatccttct acaactcaga gatccacggg attagtcgga
     3961 tgacccagac acctcagagg gttggggggg tgtggccttg ctcttcagag agggcagatc
     4021 tacttaggga gatctcttgg ggaagaaaag tggtaggcac gacagttcct cacccttctg
     4081 agatgttggg attacttccc aagtcctcta tttcttgcac ttgtggagca acaggaggag
     4141 gcaatcctag agtttctgta tcagtactcc cgtcctttga tcagtcattt ttttcacgag
     4201 gccccctaaa gggatacttg ggctcgtcca cctctatgtc gacccagcta ttccatgcat
     4261 gggaaaaagt cactaatgtt catgtggtga agagagctct atcgttaaaa gaatctataa
     4321 actggttcat tactagagat tccaacttgg ctcaagctct aattaggaac attatgtctc
     4381 tgacaggccc tgatttccct ctagaggagg cccctgtctt caaaaggacg gggtcagcct
     4441 tgcataggtt caagtctgcc agatacagcg aaggagggta ttcttctgtc tgcccgaacc
     4501 tcctctctca tatttctgtt agtacagaca ccatgtctga tttgacccaa gacgggaaga
     4561 actacgattt catgttccag ccattgatgc tttatgcaca gacatggaca tcagagctgg
     4621 tacagagaga cacaaggcta agagactcta cgtttcattg gcacctccga tgcaacaggt
     4681 gtgtgagacc cattgacgac gtgaccctgg agacctctca gatcttcgag tttccggatg
     4741 tgtcgaaaag aatatccaga atggtttctg gggctgtgcc tcacttccag aggcttcccg
     4801 atatccgtct gagaccagga gattttgaat ctctaagcgg tagagaaaag tctcaccata
     4861 tcggatcagc tcaggggctc ttatactcaa tcttagtggc aattcacgac tcaggataca
     4921 atgatggaac catcttccct gtcaacatat acggcaaggt ttcccctaga gactatttga
     4981 gagggctcgc aaggggagta ttgataggat cctcgatttg cttcttgaca agaatgacaa
     5041 atatcaatat taatagacct cttgaattgg tctcaggggt aatctcatat attctcctga
     5101 ggctagataa ccatccctcc ttgtacataa tgctcagaga accgtctctt agaggagaga
     5161 tattttctat ccctcagaaa atccccgccg cttatccaac cactatgaaa gaaggcaaca
     5221 gatcaatctt gtgttatctc caacatgtgc tacgctatga gcgagagata atcacggcgt
     5281 ctccagagaa tgactggcta tggatctttt cagactttag aagtgccaaa atgacgtacc
     5341 tatccctcat tacttaccag tctcatcttc tactccagag ggttgagaga aacctatcta
     5401 agagtatgag agataacctg cgacaattga gttctttgat gaggcaggtg ctgggcgggc
     5461 acggagaaga taccttagag tcagacgaca acattcaacg actgctaaaa gactctttac
     5521 gaaggacaag atgggtggat caagaggtgc gccatgcagc tagaaccatg actggagatt
     5581 acagccccaa caagaaggtg tcccgtaagg taggatgttc agaatgggtc tgctctgctc
     5641 aacaggttgc agtctctacc tcagcaaacc cggcccctgt ctcggagctt gacataaggg
     5701 ccctctctaa gaggttccag aaccctttga tctcgggctt gagagtggtt cagtgggcaa
     5761 ccggtgctca ttataagctt aagcctattc tagatgatct caatgttttc ccatctctct
     5821 gccttgtagt tggggacggg tcagggggga tatcaagggc agtcctcaac atgtttccag
     5881 atgccaagct tgtgttcaac agtcttttag aggtgaatga cctgatggct tccggaacac
     5941 atccactgcc tccttcagca atcatgaggg gaggaaatga tatcgtctcc agagtgatag
     6001 atcttgactc aatctgggaa aaaccgtccg acttgagaaa cttggcaacc tggaaatact
     6061 tccagtcagt ccaaaagcag gtcaacatgt cctatgacct cattatttgc gatgcagaag
     6121 ttactgacat tgcatctatc aaccggatca ccctgttaat gtccgatttt gcattgtcta
     6181 tagatggacc actctatttg gtcttcaaaa cttatgggac tatgctagta aatccaaact
     6241 acaaggctat tcaacacctg tcaagagcgt tcccctcggt cacagggttt atcacccaag
     6301 taacttcgtc tttttcatct gagctctacc tccgattctc caaacgaggg aagtttttca
     6361 gagatgctga gtacttgacc tcttccaccc ttcgagaaat gagccttgtg ttattcaatt
     6421 gtagcagccc caagagtgag atgcagagag ctcgttcctt gaactatcag gatcttgtga
     6481 gaggatttcc tgaagaaatc atatcaaatc cttacaatga gatgatcata actctgattg
     6541 acagtgatgt agaatctttt ctagtccaca agatggttga tgatcttgag ttacagaggg
     6601 gaactctgtc taaagtggct atcattatag ccatcatgat agttttctcc aacagagtct
     6661 tcaacgtttc caaaccccta actgacccct cgttctatcc accgtctgat cccaaaatcc
     6721 tgaggcactt caacatatgt tgcagtacta tgatgtatct atctactgct ttaggtgacg
     6781 tccctagctt cgcaagactt cacgacctgt ataacagacc tataacttat tacttcagaa
     6841 agcaagtcat tcgagggaac gtttatctat cttggagttg gtccaacgac acctcagtgt
     6901 tcaaaagggt agcctgtaat tctagcctga gtctgtcatc tcactggatc aggttgattt
     6961 acaagatagt gaagactacc agactcgttg gcagcatcaa ggatctatcc agagaagtgg
     7021 aaagacacct tcataggtac aacaggtgga tcaccctaga ggatatcaga tctagatcat
     7081 ccctactaga ctacagttgc ctgtgaaccg gatactcctg gaagcctgaa gggcgaattc
     7141 tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga
     7201 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     7261 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     7321 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     7381 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     7441 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     7501 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     7561 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     7621 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     7681 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     7741 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     7801 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     7861 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     7921 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7981 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     8041 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     8101 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     8161 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     8221 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     8281 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     8341 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
     8401 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     8461 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     8521 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     8581 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     8641 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     8701 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
     8761 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     8821 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     8881 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8941 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9001 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     9061 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     9121 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     9181 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     9241 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     9301 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9361 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     9421 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9481 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9541 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9601 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9661 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9721 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9781 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9841 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     9901 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9961 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    10021 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    10081 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    10141 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    10201 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    10261 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    10321 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    10381 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    10441 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    10501 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    10561 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10621 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10681 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10741 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    10801 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    10861 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10921 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10981 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    11041 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    11101 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    11161 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
    11221 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
    11281 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
    11341 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
    11401 cttcgcgatg tacgggccag atatacgcgt t
//
