LOCUS       sequence-204100-       10932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses murine phospho-mimetic CEP215 at S608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10932)
  AUTHORS   Colicino EG, Garrastegui AM, Freshour J, Santra P, Post DE, Kotula 
            L, Hehnly H
  TITLE     Gravin regulates centrosome function through PLK1.
  JOURNAL   Mol Biol Cell. 2017 Dec 27. pii: mbc.E17-08-0524. doi: 
            10.1091/mbc.E17-08-0524.
  PUBMED    29282278
REFERENCE   2  (bases 1 to 10932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2017 Dec 27. pii: mbc.E17-08-0524. doi: 
            10.1091/mbc.E17-08-0524."
COMMENT     Sequence Label: (PM-S608E)FLAG-Cep215-FL
FEATURES             Location/Qualifiers
     source          1..10932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(32..51)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        223..602
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        603..806
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     757..777
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     851..870
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        851..869
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      883..892
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             892..963
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     promoter        complement(6473..6491)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6474..6491)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6511..6528)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6517..6741
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6787..7215
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6874..6893)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7084..7105
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7224..7244)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7229..7558
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7409..7544
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7471..7490
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7625..8419
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7679..7698)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8289..8308
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8593..8714
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8630..8649)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8684..8703
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8763..8779)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8763..8779)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8776..8798)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8787..8803
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8811..8817,8818..8835,8836..8841))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8856..8877
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8994..9011)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9165..9753)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9245..9264)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9924..10715,10716..10784))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10547..10566
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10785..10889)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
       61 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
      121 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
      181 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
      241 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      301 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      361 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      421 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      481 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      541 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      601 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      661 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      721 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      781 ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact gcttactggc
      841 ttatcgaaat taatacgact cactataggg agacccaagc ttgccaccat ggattacaag
      901 gatgacgacg ataaggatta caaggatgac gacgataagg attacaagga tgacgacgat
      961 aagggatcta tggactcggg gatggaagag gaaggcgccc tgcctgggac cctcagcggt
     1021 tgcagtggcc ttcaccctgt tctgccaagt gacctggatg tcatcagtga cacttctggc
     1081 ttgggaaatg gagtgctccc aagcatgtca gaagaaaagg tttctcccac cagagcccgg
     1141 aacatgaagg actttgaaaa ccaaatcact gagctgaaga aggagaactt caatctaaag
     1201 ctccggatct acttcctgga ggagagaatc cagcaggagt ttgctggccc caccgagcac
     1261 atctacaaga cgaacattga actcaaggtg gaagtggaaa gtctgaagcg cgagctccag
     1321 gagaaagatc agttgctcgt caaagcctcc aaagcggtgg agagcctagc agaacggggt
     1381 ggttctgaag tccagcgggt gaaagaagat gcaaggaaga aggtgcagca ggtggaagac
     1441 ctcctaacga aaaggataca tcttctagaa gaagatgtga aagccgccca ggcagaactg
     1501 gagaaggcct ttgcggggac agaaacagag aaggctcttc ggctcagctt agaaagcaag
     1561 ctctcggcga tgaagaagat gcaggagggg gacctggaga tgactctggc tctggaagag
     1621 aaggacagac tgattgagga gttgaagctg tctttgaaga gcaaagaagc tttaattcag
     1681 tgccttaaag aggaaaaatc tcagatggca tctcctgacg agaacgtgtc atctggagaa
     1741 ctccgaggac tttctgccac tctgagggaa gaaaaagaga gagacgctga ggaatggcag
     1801 aaggagagaa accactttga agagaggatc caggcacttc aggaggacct gagagagaag
     1861 gaaagagaaa ttgctacaga gaagaaaaat aacttaaaga gttataaagc cattcagggt
     1921 ctaaccatgg cattaaaatc aaaggaaaga gaggttgaag aacttgattc caaaatcaaa
     1981 gaggtcacta cggactccac caaaggcaga gaggatccct taaaaaccca gatccccaga
     2041 ttccagctaa gagaggggtc tgaggactgt gaagctgcgc tggtagagaa ggaagcccta
     2101 ttggccaagc tacactcaga aaatgtcaca aagagcactg agaaccatag gttgctcagg
     2161 aatgttaaaa aggtcaccca ggagttgaat gacttgaaga aggagaagtt gagactagag
     2221 caggccctgg aggaagccca ccaagagggg aacagaggag cccgaaccat tcacgacctt
     2281 agaaatgaag ttgagaaact acgcaaggaa gtgagtgaga gagagaaggc tgtggaaaaa
     2341 cattacaaga gtcttccagg tgaaagcaag acaaagtttc atactcaaga acaagtggtc
     2401 agaagtctga caggcagtgg cagtcaggag gacctgctgc ttcagaaatc caatgaaaaa
     2461 gatttggaag caatacagca gaactgctat ttaatgactg cagaggagct caagtttggg
     2521 agcgatggct taataacaga aaagtgttct cagcagtcac cagacagcaa actcatcttc
     2581 tccaaggaaa agcaacaatc agagtatgaa gggctgactg gggacttaaa agcagaacag
     2641 aacatctatg cccatctggc aaagactcag gacacagaca gtgtcagcaa cctgcaggct
     2701 gagctgaaag aggtccttgc ccttcggaag cagctggagc aggatgttct tgcctaccgg
     2761 aacctgcaga aggccctgca ggagcagctc gaggagattc ggagtcgaga agaagagccg
     2821 ttttcatttt atagcgatca aacatcgtat ctgagtattt gccttgaaga gcacaatcag
     2881 ttccaattgg agcatttttc tcaagaagaa ctcaagaaaa aggtcagtga cctcatacag
     2941 cttgtgaagg acctgcacac agacaaccag cacctgaaga aaaccatttt tgatctctct
     3001 tctgtgggtt tccagggaag tgacagactt gagttaacaa aacaagaaga gcttgtggct
     3061 agcaaggagg atgaggacac actcaaattt gaagcggacg ttgagactcc tttccagagt
     3121 gaccagcatt tggagcaaag tcgtgagatc atggaggact atgctgaggg tggctgcaaa
     3181 agtggatatg gaaggcacat ggattccaat attttaggtc atgatggggc ccaaactcct
     3241 ggtgcctctg aagagcacac cctagaagat gagctcctgg gcctgcttgc cactctcttc
     3301 agtaagaagg ccacaccgtt gctggaatcc aggccagatc ttctgaaagc actcggtgcc
     3361 ctacttttgg aacgcatatg cttggcagag cagggtcgtc ctggagatca ccttgatagt
     3421 aaaactgaga aggcacttca gcaagtagct gttcagttga gagatgaact tggccattcc
     3481 ttccctgcca actcgttctc caagtcatac aatgaagtca agtcaatgtg gggaaattgg
     3541 ctggtaaaga caggagacga agacacggtg gagctgaaga gtgtttcagt gcagaccatg
     3601 gccatagagg acaccccgca tggattcaaa cctcaaagca agagagatgc ttgggcagag
     3661 aaacaagaag aggccatttt cagcactgag cttgagtcag aggcgttggg tgagatgcca
     3721 gggcaacagg ccactcacct ctcattcccc agtgccatca acccggatgc cgagaagacg
     3781 ggcttgttga tccaattaaa gacacctgag ttattggaaa atttatataa tcttcctgct
     3841 tcgccagagg tggtggtggc tcaactccaa ggccaggttc tagagctgca gagggagctg
     3901 aaagaattca aaacccgcaa caagcaactc catgagaagc taattctggc agaggcaatg
     3961 atggaggggc tgccggtgcc caactctgcg ctggtgaatg tgcctgcagc ccaggctgtt
     4021 gtgagaacag ccttccagga caacccaggc gagcaagaag gacctgaaac cacacagtct
     4081 gctgggagag acaaggacat ggacagtgac caatatacaa gctttgagat cgattctgaa
     4141 atctgtccac ctgatgacct tgccttatta ccagcatgca aagagaatct tgaagacctc
     4201 ctaggcccat cctccatagc cacttacctg gattccaaga gtcagctctc tgttaaagtc
     4261 agcgtgaatg gaactgacca gtctgagaat atcaatatcc ctgatgacac agaagactta
     4321 aaacagaaaa tccacgactt gcaaaccgag ctggaaggct accggaacat cattgtccag
     4381 ctactgaagc actctcagtg cagtgaggct attatcacag ttttgtgtgg gacagaaggg
     4441 gcccaagatg gcttgaacaa gcctaagggc catattgatg aggaagagat gacattttca
     4501 agtttgcacc aagtacgata tgtgaagcat atgaaaatcc tccgtccact aaccccggag
     4561 atgattgatg gcaagatgct agagagcctc aagcagcagc tggtggacca ggagcaggag
     4621 ctacagaagg agcaggactt gaacttggaa cttttcggtg aaatccatga cctgcagaac
     4681 aagttccaag acctctcgcc atcaagatac gattcattgg ttcagtccca agcgagggag
     4741 ctctcccttc agcgacagca aattaaggat agccatggca tctgtgtcat ttaccgtcaa
     4801 cacatgagca ccatgatcaa ggcttttgag gagcttctgc aggccagtga tgtggacagt
     4861 tgcgtggccg agggcttccg ggagcagctg acacagtgtg ctggactgct agagcaactg
     4921 gagaggctgt ttctccatgg aaaatcagcc agagtggaac cacatcccca gaatgaactg
     4981 ctcaaggggt taaggactgt ggaaggcaac ttaccctacc accatctcct gcctgaatct
     5041 cctgagcctt ctgcctccca tgctctgtca gatgatgaaa tgtctgagaa gtccttcctg
     5101 tctagagacc cgaagccaga cagcgatact gagaagtacc cagcaatggc gagccatttt
     5161 cctcaagact tactaatgga acacatacag gaaattcgaa ctttgagaaa gcatttggaa
     5221 gaatctatta aaacaaatga gaagctccgg aaacagctgg aacggcaggg gtctgagact
     5281 gaccaaggct ccagaaatgt ttctgcttgt ggcttagcac tgcacagctc tctgacgtca
     5341 gagatacatt tcctgaggaa gcagaacgag gccctcagta tgatgctcga gaaggggtcc
     5401 aaagataaac agaaagagag tgaaaaatta cgtgagtccc tggctaggaa agctgagagt
     5461 cttgagcaac ttcagctaga gtacaccagt gtaagggaag aaaatgaaag gttacagaga
     5521 gacatcattg agaaggagag acacaaccag gagctgactg aggaggtctg cagcagccgc
     5581 caggaactga gcagggtgca ggaagaggcg aagtcaagac agcagctgct ctcacagaag
     5641 gacaagctgc ttcagtccct ccaaatggag ctcaaggtgt atgagaaact ggccgaggaa
     5701 catccgaggc tacagcaaga tggcagcaaa tgtcctgagg ccagcgacaa ttcatttgat
     5761 ctatttgaat ccacccaggc aatggcacct aaatcagctt ctgagactcc tctgctctca
     5821 ggaactgatg ttgactcgct ctcctgtgac agtaccagtt ctgccaccag ccctactagc
     5881 atgccctgcc tggttgctgg ccaccacatg tgggccagca agagtggcca ccatatgctg
     5941 ggcctgattg aggactatga tgccctctat aagcagatca gctggggcca gacactgctt
     6001 gccaagatgg atgttcaaac ccaagaggct ctgagcccca caagtcacaa gctgggacca
     6061 aagggttctt catccgtgcc tctgagcaag tttctttcca gtatgaacac agccaagctg
     6121 gtcctggaaa aagcatccag gttgctgaag ctcttctgga gggtctcagt ccccaccaac
     6181 ggccagtgtt cccttcactg tgagcagatt ggagaaatga aggcagagaa cacgaaacta
     6241 cacaaaaaat tgtttgaaca agaaaagaag ctgcagaaca cagcaaaact attgcagcag
     6301 agcaagcacc aggagaaagt catctttgat cagttggtca tcacccacca agtccttcgg
     6361 aaggccaggg gaaacctgga gctcaggcct ggggccaccc gtccaggagc atccagtccc
     6421 agcagaccgg gctcatgagc ggccgctcga gcatgcatct agagggccct attctatagt
     6481 gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     6541 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     6601 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     6661 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     6721 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     6781 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6841 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     6901 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     6961 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7021 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7081 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7141 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7201 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     7261 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     7321 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7381 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7441 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     7501 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     7561 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     7621 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     7681 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     7741 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     7801 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     7861 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     7921 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     7981 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     8041 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     8101 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     8161 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     8221 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     8281 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     8341 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     8401 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     8461 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     8521 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     8581 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8641 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8701 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     8761 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8821 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8881 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8941 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     9001 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9061 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9121 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9181 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9241 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9301 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9361 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9421 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9481 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9541 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9601 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9661 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9721 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9781 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9841 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9901 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9961 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    10021 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    10081 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10141 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10201 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10261 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10321 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10381 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10441 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10501 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10561 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10621 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10681 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10741 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10801 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10861 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    10921 gacggatcgg ga
//
