LOCUS       sequence-204104-       10932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of CEP215.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10932)
  TITLE     Mammalian expression of CEP215
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FLAG-Cep215-FL
FEATURES             Location/Qualifiers
     source          1..10932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(26..45)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        217..596
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        597..800
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     751..771
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     845..864
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        845..863
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      877..886
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             886..957
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     promoter        complement(6467..6485)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6468..6485)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6505..6522)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6511..6735
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6781..7209
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6868..6887)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7078..7099
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7218..7238)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7223..7552
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7403..7538
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7465..7484
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7619..8413
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7673..7692)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8283..8302
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8587..8708
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8624..8643)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8678..8697
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8757..8773)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8757..8773)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8770..8792)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8781..8797
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8805..8811,8812..8829,8830..8835))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8850..8871
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8988..9005)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9159..9747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9239..9258)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9918..10709,10710..10778))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10541..10560
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10779..10883)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
       61 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
      121 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
      181 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
      241 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      301 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      361 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      421 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      481 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      541 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      601 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      661 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      721 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      781 aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg
      841 aaattaatac gactcactat agggagaccc aagcttgcca ccatggatta caaggatgac
      901 gacgataagg attacaagga tgacgacgat aaggattaca aggatgacga cgataaggga
      961 tctatggact cggggatgga agaggaaggc gccctgcctg ggaccctcag cggttgcagt
     1021 ggccttcacc ctgttctgcc aagtgacctg gatgtcatca gtgacacttc tggcttggga
     1081 aatggagtgc tcccaagcat gtcagaagaa aaggtttctc ccaccagagc ccggaacatg
     1141 aaggactttg aaaaccaaat cactgagctg aagaaggaga acttcaatct aaagctccgg
     1201 atctacttcc tggaggagag aatccagcag gagtttgctg gccccaccga gcacatctac
     1261 aagacgaaca ttgaactcaa ggtggaagtg gaaagtctga agcgcgagct ccaggagaaa
     1321 gatcagttgc tcgtcaaagc ctccaaagcg gtggagagcc tagcagaacg gggtggttct
     1381 gaagtccagc gggtgaaaga agatgcaagg aagaaggtgc agcaggtgga agacctccta
     1441 acgaaaagga tacatcttct agaagaagat gtgaaagccg cccaggcaga actggagaag
     1501 gcctttgcgg ggacagaaac agagaaggct cttcggctca gcttagaaag caagctctcg
     1561 gcgatgaaga agatgcagga gggggacctg gagatgactc tggctctgga agagaaggac
     1621 agactgattg aggagttgaa gctgtctttg aagagcaaag aagctttaat tcagtgcctt
     1681 aaagaggaaa aatctcagat ggcatctcct gacgagaacg tgtcatctgg agaactccga
     1741 ggactttctg ccactctgag ggaagaaaaa gagagagacg ctgaggaatg gcagaaggag
     1801 agaaaccact ttgaagagag gatccaggca cttcaggagg acctgagaga gaaggaaaga
     1861 gaaattgcta cagagaagaa aaataactta aagagttata aagccattca gggtctaacc
     1921 atggcattaa aatcaaagga aagagaggtt gaagaacttg attccaaaat caaagaggtc
     1981 actacggact ccaccaaagg cagagaggat cccttaaaaa cccagatccc cagattccag
     2041 ctaagagagg ggtctgagga ctgtgaagct gcgctggtag agaaggaagc cctattggcc
     2101 aagctacact cagaaaatgt cacaaagagc actgagaacc ataggttgct caggaatgtt
     2161 aaaaaggtca cccaggagtt gaatgacttg aagaaggaga agttgagact agagcaggcc
     2221 ctggaggaag cccaccaaga ggggaacaga ggagcccgaa ccattcacga ccttagaaat
     2281 gaagttgaga aactacgcaa ggaagtgagt gagagagaga aggctgtgga aaaacattac
     2341 aagagtcttc caggtgaaag caagacaaag tttcatactc aagaacaagt ggtcagaagt
     2401 ctgacaggca gtggcagtca ggaggacctg ctgcttcaga aatccaatga aaaagatttg
     2461 gaagcaatac agcagaactg ctatttaatg actgcagagg agctcaagtt tgggagcgat
     2521 ggcttaataa cagaaaagtg ttctcagcag tcaccagaca gcaaactcat cttctccaag
     2581 gaaaagcaac aatcagagta tgaagggctg actggggact taaaagcaga acagaacatc
     2641 tatgcccatc tggcaaagac tcaggacaca gacagtgtca gcaacctgca ggctgagctg
     2701 aaagaggtcc ttgcccttcg gaagcagctg gagcaggatg ttcttgccta ccggaacctg
     2761 cagaaggccc tgcaggagca gctcagtgag attcggagtc gagaagaaga gccgttttca
     2821 ttttatagcg atcaaacatc gtatctgagt atttgccttg aagagcacaa tcagttccaa
     2881 ttggagcatt tttctcaaga agaactcaag aaaaaggtca gtgacctcat acagcttgtg
     2941 aaggacctgc acacagacaa ccagcacctg aagaaaacca tttttgatct ctcttctgtg
     3001 ggtttccagg gaagtgacag acttgagtta acaaaacaag aagagcttgt ggctagcaag
     3061 gaggatgagg acacactcaa atttgaagcg gacgttgaga ctcctttcca gagtgaccag
     3121 catttggagc aaagtcgtga gatcatggag gactatgctg agggtggctg caaaagtgga
     3181 tatggaaggc acatggattc caatatttta ggtcatgatg gggcccaaac tcctggtgcc
     3241 tctgaagagc acaccctaga agatgagctc ctgggcctgc ttgccactct cttcagtaag
     3301 aaggccacac cgttgctgga atccaggcca gatcttctga aagcactcgg tgccctactt
     3361 ttggaacgca tatgcttggc agagcagggt cgtcctggag atcaccttga tagtaaaact
     3421 gagaaggcac ttcagcaagt agctgttcag ttgagagatg aacttggcca ttccttccct
     3481 gccaactcgt tctccaagtc atacaatgaa gtcaagtcaa tgtggggaaa ttggctggta
     3541 aagacaggag acgaagacac ggtggagctg aagagtgttt cagtgcagac catggccata
     3601 gaggacaccc cgcatggatt caaacctcaa agcaagagag atgcttgggc agagaaacaa
     3661 gaagaggcca ttttcagcac tgagcttgag tcagaggcgt tgggtgagat gccagggcaa
     3721 caggccactc acctctcatt ccccagtgcc atcaacccgg atgccgagaa gacgggcttg
     3781 ttgatccaat taaagacacc tgagttattg gaaaatttat ataatcttcc tgcttcgcca
     3841 gaggtggtgg tggctcaact ccaaggccag gttctagagc tgcagaggga gctgaaagaa
     3901 ttcaaaaccc gcaacaagca actccatgag aagctaattc tggcagaggc aatgatggag
     3961 gggctgccgg tgcccaactc tgcgctggtg aatgtgcctg cagcccaggc tgttgtgaga
     4021 acagccttcc aggacaaccc aggcgagcaa gaaggacctg aaaccacaca gtctgctggg
     4081 agagacaagg acatggacag tgaccaatat acaagctttg agatcgattc tgaaatctgt
     4141 ccacctgatg accttgcctt attaccagca tgcaaagaga atcttgaaga cctcctaggc
     4201 ccatcctcca tagccactta cctggattcc aagagtcagc tctctgttaa agtcagcgtg
     4261 aatggaactg accagtctga gaatatcaat atccctgatg acacagaaga cttaaaacag
     4321 aaaatccacg acttgcaaac cgagctggaa ggctaccgga acatcattgt ccagctactg
     4381 aagcactctc agtgcagtga ggctattatc acagttttgt gtgggacaga aggggcccaa
     4441 gatggcttga acaagcctaa gggccatatt gatgaggaag agatgacatt ttcaagtttg
     4501 caccaagtac gatatgtgaa gcatatgaaa atcctccgtc cactaacccc ggagatgatt
     4561 gatggcaaga tgctagagag cctcaagcag cagctggtgg accaggagca ggagctacag
     4621 aaggagcagg acttgaactt ggaacttttc ggtgaaatcc atgacctgca gaacaagttc
     4681 caagacctct cgccatcaag atacgattca ttggttcagt cccaagcgag ggagctctcc
     4741 cttcagcgac agcaaattaa ggatagccat ggcatctgtg tcatttaccg tcaacacatg
     4801 agcaccatga tcaaggcttt tgaggagctt ctgcaggcca gtgatgtgga cagttgcgtg
     4861 gccgagggct tccgggagca gctgacacag tgtgctggac tgctagagca actggagagg
     4921 ctgtttctcc atggaaaatc agccagagtg gaaccacatc cccagaatga actgctcaag
     4981 gggttaagga ctgtggaagg caacttaccc taccaccatc tcctgcctga atctcctgag
     5041 ccttctgcct cccatgctct gtcagatgat gaaatgtctg agaagtcctt cctgtctaga
     5101 gacccgaagc cagacagcga tactgagaag tacccagcaa tggcgagcca ttttcctcaa
     5161 gacttactaa tggaacacat acaggaaatt cgaactttga gaaagcattt ggaagaatct
     5221 attaaaacaa atgagaagct ccggaaacag ctggaacggc aggggtctga gactgaccaa
     5281 ggctccagaa atgtttctgc ttgtggctta gcactgcaca gctctctgac gtcagagata
     5341 catttcctga ggaagcagaa cgaggccctc agtatgatgc tcgagaaggg gtccaaagat
     5401 aaacagaaag agagtgaaaa attacgtgag tccctggcta ggaaagctga gagtcttgag
     5461 caacttcagc tagagtacac cagtgtaagg gaagaaaatg aaaggttaca gagagacatc
     5521 attgagaagg agagacacaa ccaggagctg actgaggagg tctgcagcag ccgccaggaa
     5581 ctgagcaggg tgcaggaaga ggcgaagtca agacagcagc tgctctcaca gaaggacaag
     5641 ctgcttcagt ccctccaaat ggagctcaag gtgtatgaga aactggccga ggaacatccg
     5701 aggctacagc aagatggcag caaatgtcct gaggccagcg acaattcatt tgatctattt
     5761 gaatccaccc aggcaatggc acctaaatca gcttctgaga ctcctctgct ctcaggaact
     5821 gatgttgact cgctctcctg tgacagtacc agttctgcca ccagccctac tagcatgccc
     5881 tgcctggttg ctggccacca catgtgggcc agcaagagtg gccaccatat gctgggcctg
     5941 attgaggact atgatgccct ctataagcag atcagctggg gccagacact gcttgccaag
     6001 atggatgttc aaacccaaga ggctctgagc cccacaagtc acaagctggg accaaagggt
     6061 tcttcatccg tgcctctgag caagtttctt tccagtatga acacagccaa gctggtcctg
     6121 gaaaaagcat ccaggttgct gaagctcttc tggagggtct cagtccccac caacggccag
     6181 tgttcccttc actgtgagca gattggagaa atgaaggcag agaacacgaa actacacaaa
     6241 aaattgtttg aacaagaaaa gaagctgcag aacacagcaa aactattgca gcagagcaag
     6301 caccaggaga aagtcatctt tgatcagttg gtcatcaccc accaagtcct tcggaaggcc
     6361 aggggaaacc tggagctcag gcctggggcc acccgtccag gagcatccag tcccagcaga
     6421 ccgggctcat gagcggccgc tcgagcatgc atctagaggg ccctattcta tagtgtcacc
     6481 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     6541 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6601 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6661 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6721 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     6781 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6841 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6901 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6961 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7021 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7081 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7141 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7201 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     7261 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     7321 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     7381 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7441 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     7501 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     7561 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     7621 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     7681 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     7741 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     7801 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     7861 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     7921 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     7981 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     8041 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     8101 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     8161 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     8221 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     8281 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     8341 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     8401 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     8461 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     8521 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     8581 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8641 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8701 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     8761 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8821 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8881 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8941 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     9001 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9061 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9121 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9181 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9241 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9301 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9361 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9421 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9481 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9541 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9601 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9661 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9721 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9781 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9841 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9901 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9961 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10021 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10081 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10141 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10201 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10261 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10321 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10381 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10441 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10501 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10561 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10621 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10681 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10741 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10801 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10861 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
    10921 tcgggagatc tc
//
