LOCUS       sequence-204111-        6157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tight affinity yellow glutamate sensor ** A newer version of this 
            sensor is available. Please see iGluSnFR3 plasmids at 
            https://www.addgene.org/browse/article/28220233/ **.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Marvin JS, Scholl B, Wilson DE, Podgorski K, Kazemipour A, Muller 
            JA, Schoch S, Quiroz FJU, Rebola N, Bao H, Little JP, Tkachuk AN, 
            Cai E, Hantman AW, Wang SS, DePiero VJ, Borghuis BG, Chapman ER, 
            Dietrich D, DiGregorio DA, Fitzpatrick D, Looger LL
  TITLE     Stability, affinity, and chromatic variants of the glutamate sensor 
            iGluSnFR.
  JOURNAL   Nat Methods. 2018 Nov;15(11):936-939. doi: 
            10.1038/s41592-018-0171-3. Epub 2018 Oct 30.
  PUBMED    30377363
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0171-3"; journalName: "Nat Methods"; date: 
            "2018-11"; volume: "15"; issue: "11"; pages: "936-939"
COMMENT     Sequence Label: pAAV.hSynapsin.SF-Venus-iGluSnFR.A184S
FEATURES             Location/Qualifiers
     source          1..6157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   77..206
                     /label=AAV2 ITR
     polyA_signal    368..489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(405..424)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     459..478
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     518..534
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     519..535
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    538..1126
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             644..655
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1053..1073
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1142..1288)
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             complement(1292..1321)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     1786..1807
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1910..1931)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     sig_peptide     complement(2900..2959)
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     regulatory      2956..2965
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2994..3441)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     repeat_region   3517..3646
                     /label=AAV2 ITR
     rep_origin      3718..4173
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3805..3824)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4015..4036
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4199..4303
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4304..4372,4373..5164)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4522..4541)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5335..5923
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5824..5843
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
       61 cgattcatta atgcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg
      121 gcgtcgggcg acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt
      181 ggccaactcc atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc
      241 tacgtagcca tgctctagga agatccttat cgattttacc acatttgtag aggttttact
      301 tgctttaaaa aacctcccac atctccccct gaacctgaaa cataaaatga atgcaattgt
      361 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
      421 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
      481 tgtatcttat catgtctgct cgaagcggcc gcccgggtcg aggtcgacgg tatcgatgcg
      541 gggaggcggc ccaaagggag atccgactcg tctgagggcg aaggcgaaga cgcggaagag
      601 gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc tggattgagg gccgaaggga
      661 cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc agccaaggaa
      721 aggacgatga tttccccgac aacaccacgg aattgtcagt gcccaacagc cgagcccctg
      781 tccagcagcg ggcaaggcag gcggcgatga gttccgccgt ggcaataggg agggggaaag
      841 cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt
      901 cagcaaacac agtgcacacc acgccacgtt gcctgacaac gggccacaac tcctcataaa
      961 gagacagcaa ccaggattta tacaaggagg agaaaatgaa agccatacgg gaagcaatag
     1021 catgatacaa aggcattaaa gcagcgtatc cacatagcgt aaaaggagca acatagttaa
     1081 gaataccagt caatctttca caaattttgt aatccagagg ttgattatcg ataagcttct
     1141 aacgtggctt cttctgccaa agcatgatga ggatgataag ggagatgatg gtgagcacca
     1201 ccagggccag gatggctgag atcaccacca ccttaaaggg caaggagtgt ggcaccacga
     1261 tgacctcctg cgtgtcctgg cccacagcat tcagatcctc ttctgagatg agtttttgtt
     1321 cgtcgacctg cagtttcagt gccttgtcat tcggttcttt gaacagtgct ttcatttcgt
     1381 ctgacagttc gaaattcatg ttcagggggt tgttaaagtt gtactccagc ttgtgcccca
     1441 ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt
     1501 cgccctcgaa cttcacctcg gcgcgggtct tgtaggtgcc gtcgtccttg aagctgatgg
     1561 tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt
     1621 ggtcggggta gcgggcgaag cactgcacgc cgtagcccag ggtggtcacg agggtgggcc
     1681 agggcacggg cagcttgccg gtggtgcaga tgagcttcag ggtcagcttg ccgttggtgg
     1741 catcgccctc gccctcgccg cgcacgctga acttgtggcc gtttacgtcg ccgtccagct
     1801 cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcatg ctccctccgg
     1861 taccgccctt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
     1921 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagcacggac tgatagctca
     1981 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
     2041 ggtcggcgag ctgcacgctg ccgtcctcca cgttgtggcg gatcttgaag ttcgccttga
     2101 tgccgttctt ctgcttgtcg gcggtgatat agacgttgtg gctcaccaga attggatttt
     2161 tgaaccactt atcaaaccat ttttccgctt caccggaggt ctgcacctga gcgatggtgt
     2221 catccatcag ctttttgaac tgcggatcat ctttacgcaa catacaaccg taggcctcct
     2281 gagactgcgg cttgccgacg atttcccagt tgtctggttt cttcgctttc gcacgttcac
     2341 cggccagcag gctgtcatcc atcataaagg caacggcacg accgctttcc agggtgcgga
     2401 aagagtcacc gtgatctttg gcgctgatga tgcgcatatt cattttttgc tcttcattca
     2461 gtttgttgag caaaacttca gaggtagtgc cggaagtgac gactacggct ttgtctttca
     2521 ggttggcaaa atctttgata tcgccaccct ttttggtcaa caggcgcgta ccgaccacga
     2581 aaatagtgtc agagaaagcc gcctgttttt ggcgttcgac gttgttggtg gtagaaccac
     2641 attcaaaatc gaaagtgccg ttttgcagca gtggaatacg gttttgtgag gtaatcggaa
     2701 tcagttttac ctgcaagtcc ggtttgttga gtttcttttt cactgcttca acaatggcgt
     2761 tggagtaatc ctgcgagtaa cccaccactt tttgctgatt gtcgtaataa gagaaaggca
     2821 ctgaagattc acggtgaccg acgacaatca caccgttttt ggcgattttg tccagcgtgc
     2881 tgcctgcggc agatctgtca ccagtggaac ctggaaccca gagcagcagt acccatagca
     2941 ggagtgtgtc tgtctccatg gtggcgggat ccttgctagc agcttgaatt ggactgcgct
     3001 ctcaggcacg acacgactcc tccgctgccc accgcagact gaggcagcgc tgagtcgccg
     3061 gcgccgcagc gcagatggtc gcgcccgtgc ccccctatct cgcgcctcgc gtggtgcggt
     3121 ccggctgggc cggcggcggc gcggacgcga ccaaggtggc cgggaagggg agtttgcggg
     3181 ggaccggcga gtgacgtcag cgcgccttca gtgctgaggc ggcggtggcg cgcgccgcca
     3241 ggcgggggcg aaggcactgt ccgcggtgct gaagctggca gtgcgcacgc gcctcgccgc
     3301 atcctgtttc ccctccccct ctctgatagg ggatgcgcaa tttggggaat gggggttggg
     3361 tgcttgtcca gtgggtcggg gccggtcgtc aggtaggcac ccccaccccg cctcatcctg
     3421 gtcctaaaac ccacttgcac tcatacgcag ggccctggat cttcctagag catggctacg
     3481 tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg
     3541 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     3601 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgg cgtaatagcg
     3661 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     3721 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     3781 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     3841 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     3901 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     3961 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4021 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     4081 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4141 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     4201 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4261 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     4321 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4381 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     4441 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4501 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     4561 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     4621 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     4681 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     4741 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4801 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     4861 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4921 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4981 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     5041 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     5101 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     5161 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     5221 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5281 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5341 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5401 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5461 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5521 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5581 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5641 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5701 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5761 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5821 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5881 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5941 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6001 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     6061 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     6121 accgcctctc cccgcgcgtt ggccgattca ttaatgc
//
