LOCUS       sequence-204124-        6168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Weak affinity yellow glutamate sensor ** A newer version of this 
            sensor is available. Please see iGluSnFR3 plasmids at 
            https://www.addgene.org/browse/article/28220233/ **.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Marvin JS, Scholl B, Wilson DE, Podgorski K, Kazemipour A, Muller 
            JA, Schoch S, Quiroz FJU, Rebola N, Bao H, Little JP, Tkachuk AN, 
            Cai E, Hantman AW, Wang SS, DePiero VJ, Borghuis BG, Chapman ER, 
            Dietrich D, DiGregorio DA, Fitzpatrick D, Looger LL
  TITLE     Stability, affinity, and chromatic variants of the glutamate sensor 
            iGluSnFR.
  JOURNAL   Nat Methods. 2018 Nov;15(11):936-939. doi: 
            10.1038/s41592-018-0171-3. Epub 2018 Oct 30.
  PUBMED    30377363
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0171-3"; journalName: "Nat Methods"; date: 
            "2018-11"; volume: "15"; issue: "11"; pages: "936-939"
COMMENT     Sequence Label: pAAV.hSynapsin.SF-Venus-iGluSnFR.S72A
FEATURES             Location/Qualifiers
     source          1..6168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(260..848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(340..359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1019..1810,1811..1879))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1642..1661
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1880..1984)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2010..2465)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2147..2168)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2359..2378
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2537..2666
                     /label=AAV2 ITR
     promoter        2742..3189
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3218..3227
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     3224..3283
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     4252..4273
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4376..4397)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             4862..4891
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4895..5041
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     misc_feature    5057..5645
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5110..5130)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5528..5539)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5648..5664)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5649..5665)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    5694..5815
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5705..5724)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5759..5778
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   5977..6106
                     /label=AAV2 ITR
ORIGIN
        1 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
       61 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      121 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      181 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      241 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      301 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      361 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      421 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      481 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      541 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      601 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      661 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      721 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      781 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      841 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      901 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
      961 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1021 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1081 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1141 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1201 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1261 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1321 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1381 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1441 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1501 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1561 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1621 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1681 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1741 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1801 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     1861 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     1921 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     1981 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
     2041 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     2101 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     2161 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     2221 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     2281 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     2341 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     2401 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     2461 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     2521 ttcgctatta cgccagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg
     2581 gcgtcgggcg acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt
     2641 ggccaactcc atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc
     2701 tacgtagcca tgctctagga agatccaggg ccctgcgtat gagtgcaagt gggttttagg
     2761 accaggatga ggcggggtgg gggtgcctac ctgacgaccg gccccgaccc actggacaag
     2821 cacccaaccc ccattcccca aattgcgcat cccctatcag agagggggag gggaaacagg
     2881 atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc ttcgcccccg
     2941 cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc actcgccggt
     3001 cccccgcaaa ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc cggcccagcc
     3061 ggaccgcacc acgcgaggcg cgagataggg gggcacgggc gcgaccatct gcgctgcggc
     3121 gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt gtcgtgcctg
     3181 agagcgcagt ccaattcaag ctgctagcaa ggatcccgcc accatggaga cagacacact
     3241 cctgctatgg gtactgctgc tctgggttcc aggttccact ggtgacagat ctgccgcagg
     3301 cagcacgctg gacaaaatcg ccaaaaacgg tgtgattgtc gtcggtcacc gtgaatcttc
     3361 agtgcctttc tcttattacg acaatcagca aaaagtggtg ggttactcgc aggattactc
     3421 caacgccatt gttgaagcag tgaaaaagaa actcaacaaa ccggacttgc aggtaaaact
     3481 gattccgatt accgcacaaa accgtattcc actgctgcaa aacggcactt tcgattttga
     3541 atgtggttct accaccaaca acgtcgaacg ccaaaaacag gcggctttct ctgacactat
     3601 tttcgtggtc ggtacgcgcc tgttgaccaa aaagggtggc gatatcaaag attttgccaa
     3661 cctgaaagac aaagccgtag tcgtcacttc cggcactacc tctgaagttt tgctcaacaa
     3721 actgaatgaa gagcaaaaaa tgaatatgcg catcatcagc gccaaagatc acggtgactc
     3781 tttccgcacc ctggaaagcg gtcgtgccgt tgcctttatg atggatgacg tgctgctggc
     3841 cggtgaacgt gcgaaagcga agaaaccaga caactgggaa atcgtcggca agccgcagtc
     3901 tcaggaggcc tacggttgta tgttgcgtaa agatgatccg cagttcaaaa agctgatgga
     3961 tgacaccatc gctcaggtgc agacctccgg tgaagcggaa aaatggtttg ataagtggtt
     4021 caaaaatcca attctggtga gccacaacgt ctatatcacc gccgacaagc agaagaacgg
     4081 catcaaggcg aacttcaaga tccgccacaa cgtggaggac ggcagcgtgc agctcgccga
     4141 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     4201 cctgagctat cagtccgtgc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     4261 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg
     4321 taccggaggg agcatgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     4381 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg cgcggcgagg gcgagggcga
     4441 tgccaccaac ggcaagctga ccctgaagct catctgcacc accggcaagc tgcccgtgcc
     4501 ctggcccacc ctcgtgacca ccctgggcta cggcgtgcag tgcttcgccc gctaccccga
     4561 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     4621 caccatcagc ttcaaggacg acggcaccta caagacccgc gccgaggtga agttcgaggg
     4681 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     4741 cctggggcac aagctggagt acaactttaa caaccccctg aacatgaatt tcgaactgtc
     4801 agacgaaatg aaagcactgt tcaaagaacc gaatgacaag gcactgaaac tgcaggtcga
     4861 cgaacaaaaa ctcatctcag aagaggatct gaatgctgtg ggccaggaca cgcaggaggt
     4921 catcgtggtg ccacactcct tgccctttaa ggtggtggtg atctcagcca tcctggccct
     4981 ggtggtgctc accatcatct cccttatcat cctcatcatg ctttggcaga agaagccacg
     5041 ttagaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     5101 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     5161 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     5221 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     5281 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     5341 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     5401 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     5461 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     5521 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     5581 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     5641 cccgcatcga taccgtcgac ctcgacccgg gcggccgctt cgagcagaca tgataagata
     5701 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     5761 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     5821 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag
     5881 caagtaaaac ctctacaaat gtggtaaaat cgataaggat cttcctagag catggctacg
     5941 tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg
     6001 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     6061 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc attaatgaat
     6121 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgc
//
