LOCUS       sequence-204136-       18973 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector: the coding sequence of M71 is replaced by the 
            sequence encoding amino acids 1-447 of Drd1a and an IRES-tauGFP 
            followed by ACNF cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18973)
  AUTHORS   Katidou M, Grosmaitre X, Lin J, Mombaerts P
  TITLE     G-protein coupled receptors Mc4r and Drd1a can serve as surrogate 
            odorant receptors in mouse olfactory sensory neurons.
  JOURNAL   Mol Cell Neurosci. 2018 Apr;88:138-147. doi: 
            10.1016/j.mcn.2018.01.010. Epub 2018 Jan 31.
  PUBMED    29407371
REFERENCE   2  (bases 1 to 18973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2018-04"; volume: "88:138-147. doi"; pages: " 
            10.1016/j.mcn.2018.01.010. Epub 2018 Jan 31"
COMMENT     Sequence Label: Drd1a->M71-IRES-tauGFP ACNF TV
FEATURES             Location/Qualifiers
     source          1..18973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2004..2021)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    5879..6430
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6023..6040)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     regulatory      7606..7615
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7612..7614,7615..7617,7618..8331)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7612..7614,7615..7617,7618..8328)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7918..7937)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8265..8286
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    8378..8411
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    8438..8486
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(8493..9296)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8604..8623)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9214..9233
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     polyA_signal    10034..10082
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     intron          10432..10497
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(11323..11343)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(11367..11378)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    12053..12086
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(16038..16054)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16039..16055)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(16079..16098)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(16080..16098)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(16105..16122)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(16105..16121)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16114..16136)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      16263..16718
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(16350..16369)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     16560..16581
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        16744..16848
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(16849..16917,16918..17709)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(17067..17086)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      17880..18468
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     18369..18388
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     18622..18639
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    18756..18777
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(18792..18797,18798..18815,18816..18822)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    18830..18846
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     18835..18857
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     18854..18870
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     18854..18870
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        18891..18909
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        18952..18970
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcgactcgat caacatgcgg atacttcatt cctccttaga atagggaaca aaataccaat
       61 ggaaggagtt acagagacaa agtttggagc taagacaaaa ggatagatca tccagagact
      121 actccaccca ggggtccatc ccataatcag ccaccaaatg cagacactat tgcacatgcc
      181 accaagattt tgctgaaagg accctgatat agctgtctct tgtgaggcta cgccagtgcc
      241 tggcaaatac agaagtggat gctcacagtc atctattgga tggaacacag ggcccccaat
      301 gaaggagcta gagaaagaac ccaaggaact gaaggggtct gcaaccctat aggttgaaca
      361 acaatataaa ctaaccagta cccccagagc tcgtgactct agctgcatat gtagcagagg
      421 atggcctagt cagccatcat tgggaagaga ggccccttga tcttgcaaac tttatatgcc
      481 ccagtacagg gcaactccag ggccaagaag tgggagtggg tgggtagggg agcagggcgg
      541 gggggggggt gtatagggaa ctttcaggat agcatttgaa atgtaaataa agaaaatatc
      601 taataaaatt taaaaaaaaa aagaaaagaa aactgtccca gaaagaacaa taaaaatatt
      661 ctattaaact tgccagactt ctcttttaag aaggctataa gggggaaagt gtagaaaaca
      721 gacacagaga aacaagatta actgacaatg tataataaca ctcatgtgta atggcactga
      781 aaatgatgtt gcaggtagtc actgggtacc tataggaatg aaaataatga taatgatgat
      841 acattggtag taatttgtag tactgatttc ctgtcagaaa agatctggaa acaatgatct
      901 tccagaagca atgagcttct ctggcatcag attcacattc ctggggtgtc atttccctac
      961 tgaaagaacc aagattcttg gtgaaatgaa tgactcagct ctgaagaatg aaaagaatgt
     1021 ggtaaagtca caacatcttg tcataccaga tggtaaggaa gatattagag actagtatat
     1081 taagttcaga gacaaattga gaagtccaga gactgtcaat acctggggca gtagcttact
     1141 ggttaagtgt gcgcactgct gttgctgagc acctgagtgt ggttctcagc acatagctca
     1201 acttataact caaggtccaa gggggaattt attgctgtcc cctccaaagg tatttgtgtt
     1261 catgtgaaca taccaacaca gacacatgta caatttaaaa taatgaaaat aaaatgtaaa
     1321 acataaaatt aaattctgat aaaagcatag taattgtatt gggctgaagc acattaaata
     1381 tgttcacact agtgaattta taaagggggg gatataagaa accaactatt gttttaaaat
     1441 tataaaacat atctttttat tataactgta atagcaaata caggaactat tctgttgaat
     1501 agatagcaca aaaataagca ccaaaatgag tgtgattaaa attggagagc tctgaataaa
     1561 attaaaacta gtgtcaatat tctggccatg atgagtacag ctgttttcaa agacattact
     1621 ctctccccgg gaaggaattt tagcaacagg cttccacttt gtgcttcaag ttaaaattgc
     1681 aaaacaaaga agccaggacc tgaatttgag aaataatgtg tgtcatttgt ctttctgtcc
     1741 ctgagttgcc tcacttggca aaactttgtt tcaacaaaac tctttaaaac aagcatgtca
     1801 aggctagctc atgatgcatc atggtgacta gctaacaata tgtaataacc atcatggcac
     1861 tgagaatgat gttgctggta gttactgtgg tgcctattga gatgaaaatg acaataatga
     1921 taatacatca gtagtaaagg tgatagccat ggtcataatg gtaatgatga taaggatggg
     1981 tgggtagtgg tgatggatgg ttggtggtgg tggtggtgat ggtagtggta ggggggtaat
     2041 ggtggtggta acggctctgt tgatgctaaa ttgttcattg ccccattata ttctaagttt
     2101 ctgaaactga aagatgactt ttacagataa aagaagaatt aacacacttg ggaaataaaa
     2161 catgattcac agaacaagag aaaacatgaa ctaattgtta ctttagagac aacaatggtc
     2221 tctagagtga ctatatccca ggagatgatc cacacacaca cacacatata tataaacaga
     2281 acccccaatt tttagtcata ttatatcacc tttccaaatg acaatctcct tttccggact
     2341 agactgggct ctttaagtaa attttgtggt gtatacacat ctgcatactt ctagaattta
     2401 ctgaatattg aagccacaga cattgcctga agtttcattt gagacgctgt ttcatttcct
     2461 tcagcacagg tattttgtct aaaaattaga tgagaaaata tcttctcttg tgagctcttc
     2521 tttatagttt agactattta tgaccatatg ttgacagtta acttttaatg ctctgttcta
     2581 tgtgatgtac tctcttcaga aaaaatcctg agcttctgaa agcttgataa taatagctaa
     2641 tacacatagg aactcagaag gtatgtgtgg tcctagttgg tcttttatat cattagaaaa
     2701 tttaagctgt acctgatagc taacactgct acctgtattt tcagcacttg gtcagctaag
     2761 gcaggagaat cattacaagc tgaaggttaa cctgggcttt ggagtgagat cttgtctcaa
     2821 aaaacagtag tgggagacct ggagaggtgg ctcagcagtt aagagcactg actgctcatc
     2881 cagaggtcat gagttcaaat cccaaaaacc atctgtaatg agatctgatg ccctcttctg
     2941 gtgtatctga agacagcaac agcaacagtg tattcataag cataaaataa ataaatacat
     3001 ctttaaaaag aatcagtagt ggggaaaaag acaagaataa aaagctgcag aagaagatta
     3061 tataagcaaa aaaaaaaatt gagactagct gtctgagctc attctacaag aatgtattct
     3121 catctacatt gcactgggta tataacattt accctgacct gatttacaaa gtattttaat
     3181 ctctgctaat ggttactgaa tatgtttctt ataatattaa gaagcagaga ttccataaag
     3241 agggataggt aatattttta attattcatc atctagataa aaatcatttt gtagccgggc
     3301 ttgtggcaca cgcctttaat cccagcactt gggaggcaga ggcaggcaga tttctgagtt
     3361 caaggccagc ctggtctaca aggtgagttc caggacagcc agggctatac agagaaactc
     3421 tgtctcaaaa attaaaaaaa gatcattttg tagtaaataa aaataatatt caagatcata
     3481 ccgatctctg atatgtcaat atgtaacttt agttaagaaa cgttctatca ctaattcaat
     3541 atgtaaaccc tacatataac acatatgttc taatattcaa aatattccaa agatgttaaa
     3601 gattctctta tacttaagaa aaaggaagtc acttgactca gtagatgaag ccattttcca
     3661 tcaaatctta aaacttgagt tttatcccta gaagctttaa gaaacaagga gaaagccaat
     3721 ttctgcaagt tgtatataca cacacaacac agcacagcac actcactata cacaaaatct
     3781 cttttcacaa aactggtact ataagtgact tttgaaagaa tgaactgaag atgtgtcaat
     3841 aattaataat tgtatcaaaa acaattacca tagtgagatt cctgctatgc cttaagaaat
     3901 gtatcccata ctgtacaggg gcagcacatg tctgcaatcc cagccagcag gaagaagaag
     3961 aaggggtaag ttcaaggaaa accaatgcta catagaaaga cttaactttc aaaaaaagaa
     4021 aaagaaaaat agaaagaaat agagaataat acctcagata tgagaattta atttaatatg
     4081 tatcacatct actaataaca acacatgctt cttaggaaaa tatactatat atgtatatat
     4141 aatatgtatg atttgacata gtgataaatg gtgcaatggt ttaggatcta aaaataatag
     4201 ctaaaattaa caacaaagtg cttgctgatc ccacaataag caactactca tcaaatatga
     4261 caggacagca gtaagaaata cagaacttgg ccctttacta agctttaagg cctctgaaac
     4321 cctaagggct ggtccttctg tcagcaagaa gcaacagtat agagaagata tgtgtgccac
     4381 agccagaata gctcagcaag tgtcaaatgc agaaaaccag ctctccaagc agggcaagaa
     4441 cagccattca caaactcctt ctctcccacc acacaggctt cccacccaga cacaatggct
     4501 cctaacactt ctaccatgga tgagactggc ctgccagtgg agagggactt ctcctttcgc
     4561 atcctcacag cctgttttct gtccctgctt atcctgtcca ctctcttagg gaataccctt
     4621 gtctgtgccg ctgtcatcag gtttcgacac ctgcggtcca aggtgaccaa cttctttgtc
     4681 atctctttag ctgtgtcaga tctcttggtg gctgtcttgg tcatgccctg gaaagctgtg
     4741 gctgagattg ctggcttttg gccctttggg tccttttgta acatttgggt agcctttgac
     4801 atcatgtgct ccacggcatc catccttaac ctctgtgtga tcagcgtgga caggtattgg
     4861 gctatctcca gccctttcca gtatgagagg aagatgactc cgaaggcagc cttcatcctg
     4921 attagcgtag catggacttt gtctgttctc atatccttca ttccagtgca gctaagctgg
     4981 cacaaggcaa aacctacatg gcccttggat ggcaatttta cttccctgga agatgccgag
     5041 gatgacaact gtgacacgag gttgagcagg acatacgcca tttcatcctc cctcataagc
     5101 ttttacatcc ccgtagccat tatgatcgtc acttacacca gtatctacag gattgcccag
     5161 aagcaaatcc ggcgcatctc agctttggag agggcagcag tccatgccaa gaactgccag
     5221 accaccacag gtaatggaaa ccctgtcgaa tgctctcaat cagaaagttc ctttaagatg
     5281 tcctttaaga gggagactaa agtcctgaag acactgtctg tgatcatggg ggtattcgtg
     5341 tgctgctggc tccctttctt catttcgaac tgtatggtgc ccttctgtgg ctctgaggag
     5401 acccagccat tctgcattga ttccatcacc ttcgatgtat ttgtgtggtt tggctgggcg
     5461 aattcctccc tgaaccccat tatttatgct tttaatgccg attttcagaa ggcattctcg
     5521 accctcttag gatgctatag actctgccct acaacgaata atgccataga gactgtaagc
     5581 atcaacaaca acggggctgt gatgttttcc agccaccatg agccccgagg ctccatctcc
     5641 aaggactgta atctggttta cctgatccct catgctgtgg gctcctctga ggacctgaag
     5701 agggaggagg ccggtggcat acctaagcca ctggagaagc tgtccccggc cttatcggtc
     5761 atattggact atgacaccga tgtctctcta gaaaagatcc aacccgttac ccacagcgga
     5821 cagcattcaa cctgatgtgg cgcgccttaa ttaagaattc nnccctcccc cccccctaac
     5881 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     5941 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     6001 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     6061 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     6121 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     6181 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     6241 agagtcaaat ggctctccta agcgtattca acaaggggct gaaggatgcc cagaaggtac
     6301 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     6361 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     6421 gatgataata tggccacaac catggctgag ccccgccagg agttcgacgt gatggaagat
     6481 catgctcagg gggactacac cctgcaagac caggagggtg acatggaccc cggcctgaaa
     6541 gagtctcccc tgcagacccc ggccgatgat ggatctgagg aaccaggctc tgaaacctct
     6601 gatgctaaga gcactccgac ggcggaagat gcgacagcac ccttagtgga tgagggagcc
     6661 cccggtgagc aggcggccgc tcaggccccc gcggagatcc cagaaggaac cgcagctgaa
     6721 gaagcaggca ttggcgacac gtccaacctg gaagaccaag ctgccggaca cgtgacccaa
     6781 gctcgcatgg tcagtaaagg caaagatggg actggacccg atgacaaaaa aaccaagggg
     6841 gcggatggta agcctggaac gaagattgcc acaccccggg gagcagcccc tccaggccag
     6901 aaaggccagg ccaacgccac ccggattcca gcaaaaacca ctcccacccc gaagacctcg
     6961 ccaggtgaat ctgggaaatc cggggaccgc agcggctaca gcagccccgg ctccccaggc
     7021 actccgggca gccgctcccg cacaccctcc ctgccgaccc cgcccacccg ggagcccaag
     7081 aaggtggcgg tggtccgcac tccccccaag tcgccgtctg cagccaagag ccgcctgcag
     7141 gccgctcccg ggcccatgcc agacctgaag aacgtcaagt ccaaaatcgg ctccacggaa
     7201 aacctgaagc accagccagg aggtggcaag gtgcagataa ttaataagaa gctggatctt
     7261 agcaacgtcc agtccaagtg tggctcaaag gataatatca aacacgtgcc aggaggcggc
     7321 agtgtgcaaa tagtctacaa accagtggat ctgagcaagg tgacctccaa gtgtggctca
     7381 ttaggcaaca tccatcataa gccaggaggt ggccaagtgg aagtgaaatc tgagaagctg
     7441 gacttcaagg atagagtcca gtcgaagatt gggtccctgg acaacatcac acacgtccct
     7501 ggcggaggga ataaaaagat cgaaacccac aagctgacct tccgcgagaa cgccaaagcc
     7561 aagaccgacc acggggcgga gatcgtgtcc ggggatccac cggtcgccac catggtgagc
     7621 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     7681 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     7741 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     7801 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     7861 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     7921 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     7981 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     8041 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     8101 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     8161 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     8221 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     8281 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc
     8341 gactctagag attcgagatc tagatatcga tgaattcata acttcgtata atgtatgcta
     8401 tacgaagtta tggatctgtc gatcgacgga tcgatccgaa caaacgaccc aacacccgtg
     8461 cgttttattc tgtcttttta ttgccgatcc cctcagaaga actcgtcaag aaggcgatag
     8521 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     8581 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg
     8641 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     8701 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc
     8761 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     8821 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     8881 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     8941 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     9001 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     9061 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg
     9121 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     9181 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     9241 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatggccga tcccatattg
     9301 gctgcacgga tcctgaacgg cagaggttac ggcagtttgt ctctccccct tccgggagcc
     9361 accttcttct ccaaccgtcc cggtcgcgct ctcggcgctt ctgaggagag aactggctga
     9421 gtgacgccct ttatagattc gcccttgtgt cccgcccctt cctttcccgc cctcccttgc
     9481 gctacggggc cgcccgcacc ggcctacacg gagcgcgcgc ggcggagttg ttgacgctag
     9541 ggctccggct ccctggttgg gtgttctttc tgacgcgaca ggaggaggag aatgttcctg
     9601 gtcctgtcgt cctcctttcg ggtttcccgt gcactcaaac cgaggactta cagaacggag
     9661 gataaagtta ggccattttt actcagcttc ggagttcagg ctcattttca gctaaagtct
     9721 ctcattagta tccccccaca cacatcggga aaatggtttg tcctacgcat cggtaatgaa
     9781 ggcggggccc ttcgggtcct ccggagcggg ttccgggggt ggggggaagg agggagggac
     9841 gggacgggcc tcgttcatga atattcagtt caccgctgaa tatgcataag gcaggcaaga
     9901 tggcgcgtcc aatcaattgg aagtagccgt tattagtgga gaggccccag gacgttgggg
     9961 caccgcctgt gctctagtag ctttacggag ccctggcgct cgatgttcag cccaagcttt
    10021 cgcgagctcg accgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc
    10081 cgctcagctt tacagtgaca atgacggctg gcgactgaat attagtgctt acagacagca
    10141 ctacatattt tccgtcgatg ttgaaatcct ttctcatatg tcaccataaa tatcaaataa
    10201 ttatagcaat catttacgcg ttaatggcta atcgccatct tccagcaggc gcaccattgc
    10261 ccctgtttca ctatccaggt tacggatata gttcatgaca atatttacat tggtccagcc
    10321 accagcttgc atgatctccg gtattgaaac tccagcgcgg gccatatctc gcgcggctcc
    10381 gacacgggca ctgtgtccag accaggccag gtatctctga ccagagtcat cctaaaatac
    10441 acaaacaatt agaatcagta gtttaacaca ttatacactt aaaaatttta tatttacctt
    10501 agcgccgtaa atcaatcgat gagttgcttc aaaaatccct tccagggcgc gagttgatag
    10561 ctggctggtg gcagatggcg cggcaacacc attttttctg acccggcaaa acaggtagtt
    10621 attcggatca tcagctacac cagagacgga aatccatcgc tcgaccagtt tagttacccc
    10681 caggctaagt gccttctcta cacctgcggt gctaaccagc gttttcgttc tgccaatatg
    10741 gattaacatt ctcccaccgt cagtacgtga gatatcttta accctgatcc tggcaatttc
    10801 ggctatacgt aacagggtgt tataagcaat ccccagaaat gccagattac gtatatcctg
    10861 gcagcgatcg ctattttcca tgagtgaacg aacctggtcg aaatcagtgc gttcgaacgc
    10921 tagagcctgt tttgcacgtt caccggcatc aacgttttct tttcggatcc gccgcataac
    10981 cagtgaaaca gcattgctgt cacttggtcg tggcagcccg gaccgacgat gaagcatgtt
    11041 tagctggccc aaatgttgct ggatagtttt tactgccaga ccgcgcgcct gaagatatag
    11101 aagataatcg cgaacatctt caggttctgc gggaaaccat ttccggttat tcaacttgca
    11161 ccatgccgcc cacgaccggc aaacggacag aagcattttc caggtatgct cagaaaacgc
    11221 ctggcgatcc ctgaacatgt ccatcaggtt cttgcgaacc tcatcactcg ttgcatcgac
    11281 cggtaatgca ggcaaatttt ggtgtacggt cagtaaattg gacaccttcc tcttcttctt
    11341 gggcatggcc gcaggaaagc agagccctga agctcccatc accggccaat aagagccaag
    11401 cctgcagtgt gacctcatag agcaatgtgc cagccagcct gaccccaagg gccctcaggc
    11461 ttgggcacac tgtctctagg accctgagag aaagacatac ccatttctgc ttagggccct
    11521 gaggatgagc ccaggggtgg cttggcactg aagcaaagga cactggggct cagctggcag
    11581 caaagtgacc aggatgctga ggctttgacc cagaagccag aggccagagg ccaggacttc
    11641 tcttggtccc agtccaccct cactcagagc tttaccaatg ccctctggat agttgtcggg
    11701 taacggtgga cgccactgat tctctggcca gcctaggact tcgccattcc gctgattctg
    11761 ctcttccagc cactggctga ccggttggaa gtactccagc agtgccttgg catccagggc
    11821 atctgagcct accaggtcct tcagtacctc ctgccagggc ctggagcagc cagcctgcaa
    11881 cacctgcctg ccaagcagag tgaccactgt gggcacaggg gacacagggt ggggcccaca
    11941 acagcaccat tgtccacttg tccctcacta gtaaaagaac tctagggttg cggggggtgg
    12001 gggaggtctc tgtgaggctg gtaagggata tttgcctggc ccatggagat ccataacttc
    12061 gtataatgta tgctatacga agttataagc tttcgcgagc tcgaatcgga tccgaattct
    12121 taattaaggt accggcgcgc ctttaattaa aaatgctgat ctttcttcat gtaaaagtaa
    12181 gtggttatgc acacctgtat gataacctct gcaagctcac ccaactctga acaggaaaca
    12241 ggaatcattg ctctccattg ttgggctcaa ggccaatctc cttgtcctca ttctcatctt
    12301 ctctaccatt ggttctattt ataattactc gattttaaga taatttttat attattgggt
    12361 ctctgtcctc tatatgaggc caatggtaag caattttata atttttacta ttaatatttc
    12421 atctattata taatgtattt gatcatatcc accccaccct cttcttctct ttcattatca
    12481 ccccctatat atctacctcc cattttcatg tcctcttttt tttttaataa ccaactgcat
    12541 ccaattaata ctgtttatat tcacattagt gtctggccat gcatgggagc atgggtaacc
    12601 tactagcaaa tctcacacaa attcatttaa ttagtggaat ctaaattact tctagggtac
    12661 tagttgcaag tgattctgag aaatgcagtt tccagcttgt tgacactaaa tagaatgtgt
    12721 attaaagggt tagtaggaca gtctcactta aatagctttt tactcatttt cttctgacct
    12781 ttccccaaca aaagaagaaa ttgctaccac tgttgatatg ctctaattag aactaaaggt
    12841 aggaaactat ctcagaggga gacctctcga tgcaacaaca acaacaacaa caacaaaaag
    12901 gcaaaagtgt aattctgagt tagaatacat gcctgaagag ggtgaggcac tgaatttgac
    12961 gtgatgtaat cagtttcaca atttacttat attggtttta aatattgtaa tctgcaaagt
    13021 gggcaaaacc agggaaagat aattctgtgt tccagacaag acagaacaat cagatccaaa
    13081 tctgtagagc aggttatttc cacagaaaca tagtgcttct cagttcaagc tgctcgccca
    13141 aataaagctc tggagaggcg acttgtgaag catggaacac ctcactagac tgcccatgga
    13201 ttttagccca agaaaagtct ttgtcaccag agctaggaca tttagtttta tttcctggtt
    13261 agtcatgaat tttccctctt aaactttacc ttttcataga gttgactcca ctatacttag
    13321 aaccacaaac tctcactgtc aacaacagca tcctcattgt gaataccttc catggaaaga
    13381 ccagaagtct tttctcttta gccttgactt tacttcagtt tattacaagc acaaatgtat
    13441 ttttttcatt atttggaatt gatcaatttg atggcattgt gcaaattgct ttaccaattg
    13501 gatgaatttt ctcaaaagtg tagtttttaa agagaagttt attttgaaga tctttatgaa
    13561 actgcctata atagagagga gctgtggcag actaaacacc aaaaatcttt cctctgcctt
    13621 tgtgctaaaa ctgttgtttc taacacacgc acacaaaaat ctccaacagt aggcgtacat
    13681 aatctggcat acatcactgc tcttaaaaac tttttaaact gccgggcagt ggtggtgcat
    13741 gtctttaatc ccagcatttg ggaagcagag gcaggcaaat ttctgagttc aaagccagcc
    13801 tggtctacag agagagttcc agaacagccc aggttataca acgaaaccct gtctcaaata
    13861 aataaataaa taaataaata aataaataac cttttaaact aatgttagta tcttcatgat
    13921 ctcaaactga tttaggaaaa taacgtgtcc ttctttagtg tttggtatag agagtatagg
    13981 ctcttcacta aatatttaca ggaatccttt gtgaataact tcaaagtata caaggccatt
    14041 cctaaattta taagcttgca ctatagattt ttgttgaact gtagtcacac atcttgtggt
    14101 tttcaaaaat agtatatcta acttatgaaa tgtattatta gcccttttct tcttttcccc
    14161 ttcttttctc atgactgaag gtatgaagcc agttcaatga tgtgttcatc tgtgtactaa
    14221 atactgatag tgtttgtgtg cacaaaggct aaaaaggcaa aaatttttgg aagaagagtt
    14281 tgctctacct gaaacctgta aaatacagtc tttaaatgac tatgtaacag tgaaaggttc
    14341 ttttgtacta agcacttaga aggtgagtga caacaatgta ggagaaaact aatttaaggt
    14401 catttccagt ccttcctctt agccactcaa gagtacaact gtccatcact cctaaaatgt
    14461 ccaccctctt gaacatctgt gaaattccct ttttccctta attaccttct aaatagtctc
    14521 aattcctcac atataccttc tattttattt tttgcttctg gtcttcacta ggacactgtg
    14581 tctgacagtt tggccttgcc caccacattc acatagctgt cgagtaatgg ctccacatca
    14641 cccaagcctt ccccagcatc tccactgtca acaagctgat gccttttatg gacatgttaa
    14701 gggaaacttt cagcagtgat ttttttgtag gttttcatct aagggttagg tcttatgaag
    14761 tttcctcaat ctacatttat cagctgatgt cattacacag gtcttgttga ggttgtcaca
    14821 attttatagg tgcagcttct tcgttgccat atccaaagat gctatctcat agcaggtagc
    14881 cggttcctct agctcttata atctttccac cactcttcca tgatgatccc tgagacttaa
    14941 atatagtgtc tacatgtagt tgcgaatatc tgccatagtg tcagatatga atcccctcct
    15001 agttagcaag ccttaagact ttgaggttgt tgccattccc tagctactgt gagtaaagtg
    15061 gaaatgaaca cagtcaagca agtatctgtt gaggagaaca cccagtcctt tggcaatata
    15121 ccaaagaaga atacagctag atcctgtgat aatccagtta taactttctg tgaatcctcc
    15181 acagtgatta ccatagaggc ttccctattt gcaaccctag caacaatcaa taagtctttt
    15241 tgcctttctc tgaatcctag acatttgttt cagttgtttt cttaatccta accatctgac
    15301 tggagtaagg caaaatctaa aagcagtttt aatttgcatt ttcatagttg caaaggaagt
    15361 taaacacatt tttacatatt tgtcagccat ttttatttct tcctctgaga tctttctctt
    15421 caaatccagg gcctattagg ggtggggagt ggtcagttaa tgaccttaat gactgagcaa
    15481 atggagtctt tctcagaaag tcttttacta catctctatc ttagagggga ctgcctgtgt
    15541 actcttcaag tggttttagc atttaacgtt gcccatggaa gtttttgatc catttggaat
    15601 ttttgtacca aataatagat gtgggactat ttttattctt ctacatgtgg acatccagtt
    15661 ttaccagcac aatttgttga agagtttgtc tttataccag tgtatatgtt taacattttt
    15721 gtcaaatatc agatggctaa actcatgttt cagttttctg ttctgttact ctgatttaaa
    15781 tgcctgttgg tttggccatt tattttgctt tgttttgttg ccagtaccat gcaattttta
    15841 ttactatagc tctataacac tggttgaaac tgtgttctat ctctctagca ttgttctttc
    15901 tgctcaggat ttctttggtt atccttagtc tcttatggtt ccatatggat tttaggacag
    15961 ttttattatt tctgtgaaga tgttgtggag atttttagtg ggaattaatg tttaaacgaa
    16021 ttcgatatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc
    16081 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
    16141 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
    16201 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
    16261 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
    16321 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
    16381 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
    16441 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
    16501 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
    16561 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
    16621 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
    16681 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
    16741 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    16801 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
    16861 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
    16921 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
    16981 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
    17041 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
    17101 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
    17161 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
    17221 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
    17281 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
    17341 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
    17401 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
    17461 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
    17521 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
    17581 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    17641 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    17701 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    17761 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    17821 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    17881 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    17941 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    18001 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    18061 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    18121 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    18181 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    18241 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    18301 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    18361 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    18421 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    18481 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    18541 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    18601 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    18661 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
    18721 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
    18781 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
    18841 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcga aattaaccct
    18901 cactaaaggg aacaaaagct ggagctccac cgcggtggcg gccgcgagct caattaaccc
    18961 tcactaaagg gag
//
