LOCUS       sequence-20414-e         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG423GAL-ccdB
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(121..245)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(278..294)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(279..295)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      297..544
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(313..331)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(562..581)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(563..581)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(591..608)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(591..607)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(600..622)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
       61 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
      121 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
      181 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
      241 gtggtgatgg gctgcaggaa ttcgatatca agcttatcga taccgtcgac ctcgagtcat
      301 gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc taaccgaaaa
      361 ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt
      421 attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc
      481 atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat
      541 ttgcggccgg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
      601 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      661 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      721 cagttgcgc
//
