LOCUS       sequence-204143-        7212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a soma-targeted GtACR1 in mammalian neurons under the 
            CaMKIIa promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Mahn M, Gibor L, Patil P, Cohen-Kashi Malina K, Oring S, Printz Y, 
            Levy R, Lampl I, Yizhar O
  TITLE     High-efficiency optogenetic silencing with soma-targeted 
            anion-conducting channelrhodopsins.
  JOURNAL   Nat Commun. 2018 Oct 8;9(1):4125. doi: 10.1038/s41467-018-06511-8.
  PUBMED    30297821
REFERENCE   2  (bases 1 to 7212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-10-8"; volume: "9(1):4125. doi"; pages: " 
            10.1038/s41467-018-06511-8"
COMMENT     Sequence Label: pAAV-CKIIa-stGtACR1-FusionRed
FEATURES             Location/Qualifiers
     source          1..7212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        149..1437
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             2418..3113
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     misc_feature    3339..3927
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3392..3412)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3810..3821)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3959..4435
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4158..4177)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4475..4615
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4475..4604
                     /label=AAV2 ITR
     rep_origin      4690..5145
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4777..4796)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4987..5008
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5162..5181)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5281..5303
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5341..5359)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5427..5531
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5532..5600,5601..6392)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5750..5769)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6563..7151
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7052..7071
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtttaaca ttatggcctt aggtcacttc atctccatgg
      181 ggttcttctt ctgattttct agaaaatgag atgggggtgc agagagcttc ctcagtgacc
      241 tgcccagggt cacatcagaa atgtcagagc tagaacttga actcagatta ctaatcttaa
      301 attccatgcc ttgggggcat gcaagtacga tatacagaag gagtgaactc attagggcag
      361 atgaccaatg agtttaggaa agaagagtcc agggcagggt acatctacac cacccgccca
      421 gccctgggtg agtccagcca cgttcacctc attatagttg cctctctcca gtcctacctt
      481 gacgggaagc acaagcagaa actgggacag gagccccagg agaccaaatc ttcatggtcc
      541 ctctgggagg atgggtgggg agagctgtgg cagaggcctc aggaggggcc ctgctgctca
      601 gtggtgacag ataggggtga gaaagcagac agagtcattc cgtcagcatt ctgggtctgt
      661 ttggtacttc ttctcacgct aaggtggcgg tgtgatatgc acaatggcta aaaagcaggg
      721 agagctggaa agaaacaagg acagagacag aggccaagtc aaccagacca attcccagag
      781 gaagcaaaga aaccattaca gagactacaa gggggaaggg aaggagagat gaattagctt
      841 cccctgtaaa ccttagaacc cagctgttgc cagggcaacg gggcaatacc tgtctcttca
      901 gaggagatga agttgccagg gtaactacat cctgtctttc tcaaggacca tcccagaatg
      961 tggcacccac tagccgttac catagcaact gcctctttgc cccacttaat cccatcccgt
     1021 ctgttaaaag ggccctatag ttggaggtgg gggaggtagg aagagcgatg atcacttgtg
     1081 gactaagttt gttcgcatcc ccttctccaa ccccctcagt acatcaccct gggggaacag
     1141 ggtccacttg ctcctgggcc cacacagtcc tgcagtattg tgtatataag gccagggcaa
     1201 agaggagcag gttttaaagt gaaaggcagg caggtgttgg ggaggcagtt accggggcaa
     1261 cgggaacagg gcgtttcgga ggtggttgcc atggggacct ggatgctgac gaaggctcgc
     1321 gaggctgtga gcagccacag tgccctgctc agaagcccca agctcgtcag tcaagccggt
     1381 tctccgtttg cactcaggag cacgggcagg cgagtggccc ctagttctgg gggcagctct
     1441 agagcggtac cggatccgcc accatgagca gcattacttg tgacccagcc atctatggag
     1501 agtggagcag agagaaccag ttttgcgtcg aaaagagcct gattaccctg gacggcatca
     1561 agtacgtgca gctggtcatg gccgtggtga gcgcctgcca ggtgttcttt atggtcacta
     1621 gggcacccaa agtgccttgg gaggccatct acctgccaac cacagaaatg atcacctatt
     1681 cactggcttt cacaggcaac ggatacattc gagtggcaaa tgggaagtat ctgccttggg
     1741 ctagaatggc aagttggctg tgcacatgtc caatcatgct gggcctggtg agcaacatgg
     1801 ccctggtgaa gtacaaatcc attccactga atcccatgat gattgccgcc agcagcatct
     1861 gcacagtgtt cggaatcact gcctccgtgg tcctggaccc cctgcacgtg tggctgtact
     1921 gtttcatctc tagtattttc tttatctttg agatggtggt cgcattcgcc atttttgcca
     1981 tcactattca tgattttcag accatcggaa gtcccatgtc actgaaggtg gtcgaaaggc
     2041 tgaaactgat gagaatcgtg ttctacgtga gctggatggc ctatcctatc ctgtggagtt
     2101 tttcaagcac aggggcttgc attatgtctg agaacacttc ctctgtgctg tacctgctgg
     2161 gcgacgcact gtgcaaaaat acctatggaa tcctgctgtg ggctactacc tggggactgc
     2221 tgaacggcaa gtgggaccga gattacgtga aaggacgcaa tgtcgatggg acactgatgc
     2281 ccgaatatga acaggatctg gagaaaggca atactgaacg atatgaggat gctcgggctg
     2341 gggaaaccgc cgccaagagc aggatcacca gcgagggcga gtacatcccc ctggaccaga
     2401 tcgacatcaa cgtggtgatg gtgagcgagc tgattaagga gaacatgccc atgaagctgt
     2461 acatggaggg caccgtgaac aaccaccact tcaagtgcac atccgagggc gaaggcaagc
     2521 cctacgaggg cacccagacc atgagaatca aggtcgtcga gggcggccct ctccccttcg
     2581 ccttcgacat cctggctacc agcttcatgt acggcagcag aaccttcatc aagcaccctc
     2641 cgggcatccc cgacttcttt aagcagtcct tccctgaggg cttcacatgg gagagagtca
     2701 ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct
     2761 gcctcatcta caacgtcaag gttagagggg tgaacttccc agccaacggc cctgtgatgc
     2821 agaagaaaac actcggctgg gaggcctcca ccgagacgat gtaccctgct gacggaggac
     2881 tggaaggcgc atgtgacatg gctctgaagc tcgtgggagg aggacacctg atctgcaacc
     2941 ttgagacaac atacagatcc aagaaacccg ctacgaacct caagatgccc ggcgtctaca
     3001 acgtggacca cagactggaa agaatcaagg aggccgacga tgagacatac gtcgagcagc
     3061 acgaggtggc tgtggccaga tactctactg gtggcgctgg tgatggaggt aaacagtccc
     3121 agcctattct gaacaccaaa gagatggccc cccagagtaa accccctgag gagctggaaa
     3181 tgtcctcaat gccctcccct gtggcacctc ttcctgcaag aacagagggc gtgatcgaca
     3241 tgaggtccat gagttctatt gactctttta tctcatgcgc caccgatttt ccagaggcta
     3301 cgcggtttaa gtaagaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     3361 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3421 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3481 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     3541 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     3601 gtcagctcct ttccggaact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     3661 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     3721 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctatgttgcc acctggattc
     3781 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     3841 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     3901 ggatctccct ttgggccgcc tccccgcatc gataccgagc gctgctcgag agatctacgg
     3961 gtggcatccc tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt
     4021 gcccaccagc cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt
     4081 ctataatatt atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac
     4141 ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct
     4201 cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt
     4261 gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg
     4321 tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg
     4381 gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgat
     4441 tttgtaggta accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc
     4501 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     4561 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
     4621 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
     4681 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4741 gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct
     4801 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4861 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     4921 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4981 ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt
     5041 ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac
     5101 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
     5161 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     5221 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     5281 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     5341 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     5401 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5461 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5521 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5581 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5641 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5701 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5761 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5821 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5881 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5941 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6001 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6061 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6121 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6181 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6241 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6301 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6361 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6421 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6481 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6541 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6601 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6661 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6721 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6781 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6841 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6901 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6961 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7021 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7081 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7141 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7201 ttgctcacat gt
//
