LOCUS       sequence-204163-        8686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human FGAMS-mEos2 fusion in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8686)
  AUTHORS   French JB, Jones SA, Deng H, Pedley AM, Kim D, Chan CY, Hu H, Pugh 
            RJ, Zhao H, Zhang Y, Huang TJ, Fang Y, Zhuang X, Benkovic SJ
  TITLE     Spatial colocalization and functional link of purinosomes with 
            mitochondria.
  JOURNAL   Science. 2016 Feb 12;351(6274):733-7. doi: 10.1126/science.aac6054.
  PUBMED    26912862
REFERENCE   2  (bases 1 to 8686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-02-12- 12"; volume: "351"; 
            issue: "6274"; pages: "733-7"
COMMENT     Sequence Label: pFGAMS-mEos2
FEATURES             Location/Qualifiers
     source          1..8686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      21..609
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     510..529
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        784..1087
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1088..1291
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1242..1262
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             5395..6075
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    6197..6318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6234..6253)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6288..6307
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6325..6780)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6462..6483)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6674..6693
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6807..6911
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6913..7270
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6913..7104
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6936..6956)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7121..7256
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7305..8099
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7305..8099
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7359..7378)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7969..7988
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8287..8306)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8331..8378
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
       61 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
      121 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
      181 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
      241 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
      301 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
      361 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
      421 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
      481 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
      541 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
      601 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
      661 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
      721 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      781 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      841 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      901 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      961 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     1021 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     1081 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     1141 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     1201 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     1261 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg
     1321 ctaccggact cagatctcat gtccccagtc cttcacttct atgttcgtcc ctctggccat
     1381 gagggggcag cccctggaca cactcggagg aaactgcaag ggaaactgcc agagctgcag
     1441 ggcgtcgaga ctgaactgtg ctacaacgtg aactggacag ctgaggccct ccccagtgct
     1501 gaggagacaa agaagctgat gtggctgttt ggttgcccct tactgctgga tgatgttgct
     1561 cgggagtcct ggctccttcc tggctccaat gacctgctgc tggaggtcgg gcccaggctg
     1621 aacttctcca ccccaacatc caccaacatc gtgtcagtgt gccgcgccac tgggctgggg
     1681 cctgtggatc gtgtggagac cacccggcgc taccggctct cgtttgccca ccccccgtca
     1741 gctgaggtgg aagccattgc tctggctacc ctgcacgacc ggatgacaga gcagcacttc
     1801 ccccatccca tccagagttt ctcccctgag agcatgccgg aacccctcaa tggccctatc
     1861 aatatactgg gtgagggccg gcttgcgctg gagaaggcca accaggagct tggtctggct
     1921 ttagactctt gggacctaga cttctacacc aagcgcttcc aggagctaca gcggaacccg
     1981 agcactgtgg aggcctttga cttggcgcag tccaatagcg agcacagccg acactggttc
     2041 ttcaagggcc agctccacgt ggatgggcag aagctggtgc actcactgtt tgagtccatc
     2101 atgagcaccc aggaatcctc gaaccccaac aacgtcctca aattctgtga taacagcagt
     2161 gcaatccagg gaaaggaagt ccgattccta cggcctgagg accccacacg gccaagccgc
     2221 ttccagcaac agcaagggct gagacatgtt gtcttcacag cagagactca caactttccc
     2281 acaggagtat gcccctttag tggtgcaacc actggcacag ggggccggat tcgagatgtc
     2341 cagtgcacag gccgcggggc ccacgtggtg gctggcactg ccggctattg ctttggaaat
     2401 ctgcatattc caggttacaa tctgccctgg gaggatccaa gcttccagta tcctgggaat
     2461 tttgcccggc ccctggaggt tgccattgaa gccagtaatg gagcttctga ctatggcaac
     2521 aagtttgggg aaccagtgct ggctggcttc gcccgctcct tgggcctcca gctcccagac
     2581 ggccagcggc gtgagtggat caagcccatc atgtttagtg ggggcattgg gtccatggaa
     2641 gctgaccaca taagcaagga ggccccagag ccaggcatgg aagttgtaaa ggttggaggt
     2701 cccgtctaca ggattggagt tggaggtgga gctgcttcat ctgtgcaggt gcagggagat
     2761 aacaccagtg acctggactt tggggctgtg cagcgaggag acccggagat ggaacagaag
     2821 atgaaccgtg tgatcagggc ttgtgtggag gcccccaagg gaaaccccat ctgcagcctt
     2881 catgatcagg gcgctggtgg caatggcaat gtcctaaaag agctgagtga cccagctgga
     2941 gccatcattt acaccagccg cttccagctt ggggacccaa ccctgaatgc cctggaaatc
     3001 tggggggctg agtaccagga atcaaatgct cttctgctga ggtcccccaa ccgggacttc
     3061 ctgactcatg tcagtgcccg tgaacgttgc ccggcttgct tcgtgggcac catcactgga
     3121 gaccggagaa tagtgctggt ggacgatcgg gagtgtcctg tcagaagaaa tggccagggg
     3181 gatgcccccc cgacacccct gccaacccct gtggacctgg agctcgaatg ggtgctgggc
     3241 aagatgcctc ggaaggagtt cttcctgcag aggaagcccc ccatgctgca gcctctggcc
     3301 ttgcccccag ggctgagcgt gcaccaggct ctggagaggg ttctgaggct gcccgccgtg
     3361 gccagcaagc gctacctcac caataaggtg gaccgctccg tgggaggcct ggtggcccag
     3421 cagcagtgcg tggggcccct gcaaactcct ctggcagatg tagcggttgt ggcactgagc
     3481 catgaggagc tcataggggc tgccacagcc ttgggagaac agccagtcaa gagcctgctg
     3541 gacccaaaag tcgccgcccg gctggccgtg gccgaagccc tcaccaacct ggtgtttgct
     3601 ctggtcactg acctccggga tgtgaagtgt agcgggaact ggatgtgggc agccaagctc
     3661 ccaggggagg gcgcagcttt ggcggatgcc tgtgaggcta tggtggcagt gatggcagcc
     3721 ctgggtgtgg cagtggatgg tggcaaggac tccctcagca tggctgctcg ggttggcact
     3781 gagaccgtgc gggctcctgg gtcactggtc atctcagcct atgccgtctg cccagacatc
     3841 acagccactg tgaccccaga cctcaagcat cctgaaggga gaggccatct gctctatgtg
     3901 gctctgagcc ctgggcagca ccggctcggg ggcacagctc tggcccagtg cttctcccag
     3961 cttggggaac accctccaga cctggacctt cctgagaact tggtgcgggc cttcagcatc
     4021 actcaggggc tgctgaaaga ccgcctcctc tgctcaggcc acgatgtcag tgacggaggc
     4081 ctcgtcacat gcctgctgga gatggccttt gctggaaatt gcgggctaca ggtggatgtg
     4141 cctgtcccca gggttgatgt cctgtctgtg ctgttcgctg aggagccagg cctcgtgctg
     4201 gaggtgcagg agccagacct ggcccaggtg ctgaagcgtt accgggatgc tggcctccat
     4261 tgcctggagc tgggccacac aggcgaggcc gggccccacg ccatggtccg ggtgtcagtg
     4321 aacggggctg tggttctgga ggagcctgtt ggggagctgc gagccctctg ggaggagacg
     4381 agtttccagc tggaccggct acaggcagag cctcgctgtg tggcagagga ggaacggggc
     4441 ctgagggagc ggatggggcc cagctattgc ctgcccccca cctttcccaa agcctccgtg
     4501 ccccgtgagc ctggtggtcc cagcccccga gtcgccatct tgcgagagga gggcagtaat
     4561 ggagaccggg agatggccga tgccttccac ttagctgggt ttgaggtatg ggacgtgacc
     4621 atgcaggacc tctgctctgg ggcaattggg ctggacactt tccgtggcgt ggccttcgtg
     4681 ggcggcttca gctatgcaga tgtcctgggc tctgccaaag ggtgggcagc tgctgtgacc
     4741 tttcatccca gggctggggc tgagctgagg cgcttccgga agcggccaga caccttcagc
     4801 ctgggcgtgt gtaatggctg tcaactgctg gctctgctcg gctgggtggg aggcgacccc
     4861 aatgaggatg ctgcagagat gggccctgac tcccagccag cccggccagg ccttctgcta
     4921 cgccacaacc tgtctgggcg ctacgagtct cgctgggcca gcgtgcgtgt ggggcctggg
     4981 ccagccctga tgctgcgagg gatggagggc gccgtgctgc ccgtgtggag tgcgcacggg
     5041 gaaggttacg tagcattttc ttctccggaa ctccaagctc agattgaggc caggggcttg
     5101 gctccactgc actgggctga tgatgacggg aaccccacag agcagtaccc tctgaatccc
     5161 aatgggtccc cagggggcgt ggctggcatc tgctcctgtg atggccgcca cctggctgtc
     5221 atgcctcacc ctgagcgggc cgttaggcct tggcagtggg catggcgacc ccctccattt
     5281 gatactctga ccacctcccc ctggctccag ctctttatca atgcccgaaa ctggaccctg
     5341 gaagggagct gccagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatgagt
     5401 gcgattaagc cagacatgaa gatcaaactc cgtatggaag gcaacgtaaa cgggcaccac
     5461 tttgtgatcg acggagatgg tacaggcaag ccttttgagg gaaaacagag tatggatctt
     5521 gaagtcaaag agggcggacc tctgcctttt gcctttgata tcctgaccac tgcattccat
     5581 tacggcaaca gggtattcgc caaatatcca gacaacatac aagactattt taagcagtcg
     5641 tttcctaagg ggtattcgtg ggaacgaagc ttgactttcg aagacggggg catttgcatt
     5701 gccagaaacg acataacaat ggaaggggac actttctata ataaagttcg attttatggt
     5761 accaactttc ccgccaatgg tccagttatg cagaagaaga cgctgaaatg ggagccctcc
     5821 actgagaaaa tgtatgtgcg tgatggagtg ctgacgggtg atattcatat ggctttgttg
     5881 cttgaaggaa atgcccatta ccgatgtgac ttcagaacta cttacaaagc taaggagaag
     5941 ggtgtcaagt taccaggcta ccactttgtg gaccactgca ttgagatttt aagccatgac
     6001 aaagattaca acaaggttaa gctgtatgag catgctgttg ctcattctgg attgcctgac
     6061 aatgccagac gataagcggc cgcgactcta gatcataatc agccatacca catttgtaga
     6121 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     6181 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6241 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6301 actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
     6361 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     6421 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     6481 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     6541 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     6601 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     6661 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     6721 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     6781 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6841 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6901 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     6961 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7021 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7081 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     7141 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     7201 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     7261 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     7321 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     7381 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     7441 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
     7501 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     7561 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     7621 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     7681 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     7741 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     7801 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
     7861 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     7921 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     7981 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     8041 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     8101 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     8161 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     8221 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag
     8281 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     8341 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     8401 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     8461 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
     8521 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa
     8581 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8641 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgta
//
