LOCUS       sequence-204165-       10362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express N-terminally FLAG tagged human Bag6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10362)
  AUTHORS   Liu Y, Soetandyo N, Lee JG, Liu L, Xu Y, Clemons WM Jr, Ye Y
  TITLE     USP13 antagonizes gp78 to maintain functionality of a chaperone in 
            ER-associated degradation.
  JOURNAL   Elife. 2014;3:e01369. doi: 10.7554/eLife.01369. Epub 2014 Jan 14.
  PUBMED    24424410
REFERENCE   2  (bases 1 to 10362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014"; volume: "3:e01369. doi"; pages: " 10.7554/eLife.01369.
            Epub 2014 Jan 14"
COMMENT     Sequence Label: PRK5-FLAG-Bag6
FEATURES             Location/Qualifiers
     source          1..10362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      918..927
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             927..950
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(4365..4383)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4403..4420)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4409..4633
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4679..5107
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5121..5450
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5301..5436
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5517..6311
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5571..5590)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6181..6200
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6485..6606
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      6662..6671
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6675..6809
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6878..7235
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6878..7069
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      7086..7221
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7255..7272)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7255..7271)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7264..7286)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7484..7939
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7956..7975)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8075..8097
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8135..8153)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8221..8325
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8326..8394,8395..9186)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8544..8563)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9357..9945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9846..9865
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10099..10116
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10233..10254
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10269..10274,10275..10292,10293..10299)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10307..10323
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10312..10334
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10331..10347
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10331..10347
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc taagcttgcc gccatggact acaaggacga cgatgacaag gggtcgaccg
      961 agcctaatga tagtaccagt accgctgtgg aggagcctga cagcttggag gtgttggtga
     1021 agaccttgga ctctcaaact cgtaccttta ttgtgggggc ccagatgaat gtaaaagagt
     1081 ttaaggagca cattgctgcc tctgtcagca tcccatctga aaaacaacgg ctcatttacc
     1141 agggacgagt tctgcaagat gataagaagc ttcaggaata caatgttggg ggaaaggtta
     1201 tccacctggt ggaacgggct cctcctcaga ctcacctccc ttctggggca tcttctggga
     1261 cggggtctgc ctcagccact catggtgggg gatccccccc tggtactcgg gggcctgggg
     1321 cctctgttca tgaccggaat gccaacagct atgtcatggt tggaaccttc aatcttccta
     1381 gtgacggctc tgctgtggat gttcacatca acatggaaca ggccccgatt cagagtgagc
     1441 cccgggtacg gctggtgatg gctcagcaca tgatcaggga tatacagacc ttactatccc
     1501 ggatggagtg tcgaggaggg ccccaaccgc agcacagtca gccgcccccg cagccaccgg
     1561 ctgtgacccc ggagccagta gccttgagct ctcaaacatc agaaccagtt gaaagtgaag
     1621 cacctccccg ggagcccatg gaggcagaag aagtggagga gcgtgcccca gcccagaacc
     1681 cggagctcac tcctggccca gccccagcgg gcccaacacc tgccccggaa acaaatgcac
     1741 ccaaccatcc ttcccctgcg gagtatgtcg aggtgctcca ggagctacag cggctggaga
     1801 gtcgcctcca gcccttcttg cagcgctact acgaggttct gggtgctgct gccaccacgg
     1861 actacaataa caatcacgag ggccgggagg aggatcagcg gttgatcaac ttggtagggg
     1921 agagcctgcg actgctgggc aacacctttg ttgcactgtc tgacctgcgc tgcaatctgg
     1981 cctgcacgcc cccacgacac ctgcatgtgg tccggcctat gtctcactac accaccccca
     2041 tggtgctcca gcaggcagcc attcccatac agatcaatgt gggaaccact gtgaccatga
     2101 caggaaatgg gactcggccc cccccaaccc ccaatgcaga ggcacctccc cctggtcctg
     2161 ggcaggcctc atccgtggct ccgtcttcta ccaatgtcga gtcctcagct gagggggctc
     2221 ccccgccagg tccagctccc ccgccagcca ccagccaccc gagggtcatc cggatttccc
     2281 accagagtgt ggaacccgtg gtcatgatgc acatgaacat tcaagattct ggcacacagc
     2341 ctggtggtgt tccgagtgct cccactggcc ccctgggacc ccctggtcat ggccaaaccc
     2401 tgggacagca ggtgccaggc ttcccaacag ctccaacccg ggtggtgatt gcccggccca
     2461 ctcctccaca ggctcggcct tcccatcctg gagggccccc agtctctggg acactgcagg
     2521 gcgccggtct gggtaccaat gcctcgttgg cccagatggt gagcggcctt gtggggcagc
     2581 ttcttatgca gccagtcctt gtggctcagg ggaccccagg catggctcca ccgccagccc
     2641 ctgccactgc ttctgccagt gctggcacca ccaacacagc taccacagct ggccccgctc
     2701 ctggggggcc tgcccagcct ccacccaccc ctcaaccctc catggctgat cttcagttct
     2761 ctcagcttct ggggaacctg ctagggcctg cagggccagg ggctggaggg tctggtgtgg
     2821 cttctcccac catcactgtg gcgatgcctg gtgtccctgc ctttctccaa ggcatgactg
     2881 acttcttgca ggcaacacag acagcccctc caccaccccc acctcctcca cccccaccac
     2941 ctgccccaga gcagcagacc atgcccccac caggctcccc ttctggtggc gcagggagtc
     3001 ctggaggcct gggtcttgag agcctgtcac cggagttttt tacctcagtg gtgcagggtg
     3061 tgctcagctc cctgctgggc tccctggggg ctcgggctgg cagcagtgaa aatattgctg
     3121 ccttcataca acgcctcagt ggatccagca acatctttga gcctggagct gatggggccc
     3181 ttggattctt tggggccttg ctttctcttc tgtgccagaa cttctctatg gtggacgtag
     3241 tgatgcttct ccatgggcat ttccagccac tacaacggct ccagccccag ctgcgatcct
     3301 tcttccacca gcactacctg ggtggtcagg agcccacacc cagtaacatc cggatggcaa
     3361 cccacacatt gatcacgggg ctagaagagt atgtgcggga gagtttttcc ttggtgcagg
     3421 ttcagccagg tgtggacatc atccggacaa acctggaatt tctccaagag cagtttaata
     3481 gcattgctgc gcatgtgctg cattgcacag atagtggatt tggggcccgg ttgctggagt
     3541 tgtgtaacca aggcctgttt gaatgcctgg ccctaaacct gcactgcttg gggggacagc
     3601 agatggagct tgctgctgtt atcaatggcc gaattcgtcg tatgtctcgt ggggtgaatc
     3661 cctccttggt gagctggctg accactatga tgggactgag gcttcaggtg gtactggagc
     3721 acatgcctgt aggccctgat gccattctca gatacgttcg cagggttggt gatccccccc
     3781 agccacttcc tgaggagcca atggaagttc agggagcaga aagagcttcc cctgagcctc
     3841 agcgggagaa tgcttcccca gcccctggaa caacagcaga agaggccatg tcccgaggtc
     3901 cacctcctgc tcctgagggg ggctcccggg atgaacagga tggagcttca gctgagacag
     3961 aaccttgggc agctgcagtc cccccagaat gggtccctat tatccagcag gacattcaga
     4021 gccagcggaa ggtgaaaccg cagccccctc tgagtgatgc ctacctcagt ggtatgcctg
     4081 ccaagagacg caagacgatg cagggtgagg gcccccagct gcttctctca gaggctgtga
     4141 gccgggcagc taaggcagcc ggagctcggc ccctgacgag ccccgagagc ctgagccggg
     4201 acctggaggc accagaggtt caggagagct acaggcagca gctccggtct gatatacaaa
     4261 aacgactgca ggaagacccc aactacagtc cccagcgctt ccccaatgcc cagcgggcct
     4321 ttgctgatga tccttagctc gagcatgcat ctagagggcc ctattctata gtgtcaccta
     4381 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     4441 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     4501 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     4561 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     4621 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     4681 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4741 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4801 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4861 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4921 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4981 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     5041 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5101 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     5161 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     5221 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     5281 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5341 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     5401 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     5461 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     5521 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     5581 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     5641 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     5701 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     5761 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     5821 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     5881 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     5941 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     6001 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     6061 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     6121 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     6181 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     6241 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     6301 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     6361 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     6421 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     6481 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6541 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6601 atcttatcat gtctgtatac cgtcgaggat ccccgggtac cgagctcgaa ttcaatcgat
     6661 ggccgccatg gcccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6721 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6781 tcatcaatgt atcttatcat gtctggatcg ggaattaatt cggcgcagca ccatggcctg
     6841 aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag aaccagctgt
     6901 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6961 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     7021 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     7081 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     7141 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     7201 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct gttaacagct tggcactggc
     7261 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     7321 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     7381 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     7441 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     7501 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7561 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7621 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7681 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7741 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7801 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     7861 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     7921 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     7981 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     8041 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     8101 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     8161 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     8221 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     8281 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     8341 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     8401 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     8461 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     8521 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     8581 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     8641 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8701 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     8761 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     8821 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     8881 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     8941 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     9001 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     9061 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     9121 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     9181 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     9241 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     9301 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     9361 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     9421 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     9481 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     9541 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     9601 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     9661 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     9721 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     9781 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     9841 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     9901 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     9961 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    10021 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    10081 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
    10141 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
    10201 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
    10261 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
    10321 caatttcaca caggaaacag ctatgacatg attacgaatt aa
//
