LOCUS       sequence-20418-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG424GAL-ccdB
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(37..161)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(169..185)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(169..185)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(200..641)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(206..229)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    524..641
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(665..685)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(665..683)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(704..720)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(704..720)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(717..739)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    728..744
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 taaagtgtaa agcctggggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt
       61 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
      121 tcattttacg tttctcgttc agcttttttg tacaaacttg tgatggggga tccactagtt
      181 ctagaatccg gggttttttc tccttgacgt taaagtatag aggtatatta acaatttttt
      241 gttgatactt ttattacatt tgaataagaa gtaatacaaa ccgaaaatgt tgaaagtatt
      301 agttaaagtg gttatgcagt ttttgcattt atatatctgt taatagatca aaaatcatcg
      361 cttcgctgat taattacccc agaaataagg ctaaaaaact aatcgcatta tcatcctatg
      421 gttgttaatt tgattcgttc atttgaaggt ttgtggggcc aggttactgc caatttttcc
      481 tcttcataac cataaaagct agtattgtag aatctttatt gttcggagca gtgcggcgcg
      541 aggcacatct gcgtttcagg aacgcgaccg gtgaagacga ggacgcacgg aggagagtct
      601 tccttcggag ggctgtcacc cgctcggcgg cttctaatcc gtactagagc tccagctttt
      661 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
      721 tgtgaaattg ttatccgctc acaattccac acaacatacg agccgga
//
