LOCUS       sequence-204186-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSIREN-RetroQ vector containing shRNA sequence to Egr3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Salotti J, Sakchaisri K, Tourtellotte WG, Johnson PF
  TITLE     An Arf-Egr-C/EBPbeta pathway linked to ras-induced senescence and 
            cancer.
  JOURNAL   Mol Cell Biol. 2015 Mar;35(5):866-83. doi: 10.1128/MCB.01489-14. 
            Epub 2014 Dec 22.
  PUBMED    25535333
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01489-14"; journalName: "Mol Cell Biol"; date: 
            "2015-03"; volume: "35"; issue: "5"; pages: "866-83"
COMMENT     Sequence Label: pSirenRetroQ(puro)-shEgr3
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     151..170
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        298..797
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(333..356)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     730..749
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(818..837)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ggagattcat tgcatatacg atacaaggct gttagagaga taattagaat taatttgact
       61 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
      121 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
      181 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaggatccaa tcaattgcct
      241 gacaatcttc aagagagatt gtcaggcaat tgattttttt tacgcgtgaa ttctaccggg
      301 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
      361 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
      421 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
      481 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
      541 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
      601 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
      661 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
      721 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
      781 catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt
      841 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
      901 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
      961 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgg
//
