LOCUS       sequence-204202-        8545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To study localization and function of Tensin 2 with EGFP Tensin1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8545)
  AUTHORS   Clark K, Howe JD, Pullar CE, Green JA, Artym VV, Yamada KM, 
            Critchley DR
  TITLE     Tensin 2 modulates cell contractility in 3D collagen gels through 
            the RhoGAP DLC1.
  JOURNAL   J Cell Biochem. 2010 Mar 1;109(4):808-17. doi: 10.1002/jcb.22460.
  PUBMED    20069572
REFERENCE   2  (bases 1 to 8545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/jcb"; 
            journalName: "J Cell Biochem"; date: "2010-03-1- 1"; volume: "109"; 
            issue: "4"; pages: "808-17"
COMMENT     Sequence Label: pmCherry Tensin2
FEATURES             Location/Qualifiers
     source          1..8545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      21..609
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     510..529
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        784..1087
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1088..1291
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1242..1262
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1330..1339
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1336..2043
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1481..1499)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1749..1768
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    6056..6177
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6093..6112)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6147..6166
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6184..6639)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6321..6342)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6533..6552
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6666..6770
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6772..7129
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6772..6963
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6795..6815)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6980..7115
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7164..7958
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7164..7958
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7218..7237)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7828..7847
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8146..8165)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8190..8237
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
       61 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
      121 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
      181 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
      241 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
      301 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
      361 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
      421 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
      481 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
      541 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
      601 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
      661 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
      721 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      781 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      841 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      901 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      961 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     1021 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     1081 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     1141 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     1201 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     1261 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg
     1321 ctaccggtcg ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag
     1381 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     1441 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     1501 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     1561 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     1621 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     1681 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     1741 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     1801 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     1861 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     1921 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     1981 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     2041 aagtccggac tcagatctcg agctcaagct tcgaattcta tggagcggcg ctgggactta
     2101 gacctcacct acgtgacgga gcgcatcttg gccgccgcct tccccgcgcg gcccgatgaa
     2161 cagcggcacc ggggccacct gcgcgagctg gcccatgtgc tgcaatccaa gcaccgggac
     2221 aagtacctgc tcttcaacct ttcagagaaa aggcatgacc tgacccgctt aaaccccaag
     2281 gttcaagact tcggctggcc tgagctgcat gctccacccc tggacaagct gtgctccatc
     2341 tgcaaagcca tggagacatg gctcagtgct gacccacagc acgtggtcgt actatactgc
     2401 aagggaaaca agggcaagct tggggtcatc gtttctgcct acatgcacta cagcaagatc
     2461 tctgcagggg cggaccaggc actggccact cttaccatgc ggaaattctg cgaggacaag
     2521 gtggccacag aactgcagcc ctcccagcgt cgatatatca gctacttcag tgggctgcta
     2581 tctggctcca tcagaatgaa cagcagccct ctcttcctgc actatgtgct catccccatg
     2641 ctgccagcct ttgaacctgg cacaggcttc cagcccttcc ttaaaatcta ccagtccatg
     2701 cagcttgtct acacatctgg agtctatcac attgcaggcc ctggtcccca gcagctttgc
     2761 atcagcctgg agccagccct cctcctcaaa ggcgatgtca tggtaacatg ttatcacaag
     2821 ggtggccggg gcacagaccg gaccctcgtg ttccgagtcc agttccacac ctgcaccatc
     2881 cacggaccac agctcacttt ccccaaggac cagcttgacg aggcctggac tgatgagagg
     2941 ttccccttcc aagcctccgt ggagtttgtc ttctcctcca gccccgagaa gatcaaaggc
     3001 agcactccac ggaacgaccc ctcggtctct gtcgactaca acaccactga gccagccgtg
     3061 cgctgggact cctatgagaa cttcaaccag caccacgagg acagtgtgga tggctccttg
     3121 acccacaccc ggggtcccct ggatggcagt ccttatgccc aggtgcagcg gcctccccgg
     3181 cagacccccc cggcaccctc tccagagcct ccaccacccc ccatgctctc tgtcagcagc
     3241 gactcaggcc attcctccac gctgaccaca gagccggctg ctgagtcccc tggccggccg
     3301 ccccctacag ctgctgaacg gcaggagctg gatcgcctcc taggaggctg cggagtggcc
     3361 agtgggggcc ggggagctgg gcgcgagacg gccatcctag atgacgaaga gcagcccact
     3421 gtgggcggag gcccccacct cggagtgtat ccaggccata ggcctggcct cagccgccac
     3481 tgctcctgcc gccagggcta ccgggagccc tgcggggttc ccaatggggg ctactaccgg
     3541 ccagagggaa ccctggagag gaggcgactg gcctacgggg gctatgaggg atccccccag
     3601 ggctacgccg aggcctcgat ggagaagagg cgcctctgcc gatcgctgtc agaggggcta
     3661 tacccctacc cacctgagat ggggaaacca gccactgggg actttggcta ccgcgcccca
     3721 ggctaccggg aggtggtcat cctggaggac cctgggctgc ctgccctata cccatgccca
     3781 gcctgcgagg agaagctggc gctgcctaca gcagccttgt atggactgcg gctggagagg
     3841 gaggctggag aagggtgggc aagtgaggct ggcaagcctc tcctgcaccc agtgcggcct
     3901 gggcacccgc tgcctctgct cttgcctgcc tgtgggcatc accatgcccc gatgcctgac
     3961 tacagctgcc tgaagccacc caaggcaggc gaggaagggc acgagggctg ctcctacacc
     4021 atgtgccccg aaggcaggta tgggcatcca gggtaccctg ccctggtgac atacagctat
     4081 ggaggagcag ttcccagtta ctgcccagca tatggccgtg tgcctcatag ctgtggctct
     4141 ccaggagagg gcagagggta tcccagccct ggtgcccact ccccacgggc tggctccatt
     4201 tccccgggca gcccgcccta tccacaatct aggaagctga gctacgagat ccctacggag
     4261 gagggagggg acaggtaccc attgcctggg cacctggcct cagcaggacc tttggcatct
     4321 gcagagtcgc tggagccggt gtcctggagg gagggcccca gtgggcacag cacactgcct
     4381 cggtctcccc gagatgcccc atgcagtgct tcgtcagagt tgtctggtcc ctccacgccc
     4441 ctgcacacca gcagtccagt ccagggcaag gaaagcaccc ggcgacagga caccaggtcc
     4501 cccacctcag cgcccactca gagactgagt cctggcgagg ccttgccccc tgtttcccag
     4561 gcaggcaccg gaaaggcccc tgagctgccg tcgggaagtg ggcctgagcc tctggcccct
     4621 agcccagtct ctccgacctt ccctcccagc tcgcccagtg actggcctca ggaaaggagt
     4681 ccagggggcc actcagatgg cgccagtcct cggagccctg tgcccaccac acttcctggc
     4741 ctccgccacg ccccctggca aggccctcga ggcccccccg acagcccaga tgggtctccc
     4801 ctcactcctg tgccttccca gatgccctgg cttgtggcca gcccagagcc gcctcagagc
     4861 tcacctacac ctgctttccc cctggctgcc tcctatgaca ccaatggcct tagccagccc
     4921 ccacttcctg agaaacgcca cctgcccggg ccggggcaac agccaggacc ctggggccca
     4981 gagcaggcat catcgccagc cagaggcatc agtcaccatg tcaccttcgc acctctgctc
     5041 tcagataatg tcccccaaac cccagagcct cctacacaag agagccaaag caatgtcaag
     5101 tttgtccagg atacatccaa gttctggtac aagccacacc tgtcccgtga ccaagccatt
     5161 gccctgctga aggacaagga ccctggggcc ttcctgatca gggacagtca ttcattccaa
     5221 ggagcttatg ggctggccct caaggtggcc acaccgccac ccagtgccca gccctggaaa
     5281 ggggaccccg tggaacagct ggtccgccat ttcctcatcg agactgggcc caaaggggtg
     5341 aagatcaagg gctgccccag tgagccctac tttggcagcc tgtccgcctt ggtctcccag
     5401 cactccatct cccccatctc cctgccctgc tgcctgcgca ttcccagcaa agatcctctg
     5461 gaagagaccc cagaggctcc agtgcccacc aacatgagca cagcggcaga cctcctgcgt
     5521 cagggtgctg cctgcagcgt gctctacttg acctcagtgg agacagagtc actgacgggc
     5581 ccccaagctg tggcccgggc cagctctgca gctctgagct gtagcccccg cccgacacca
     5641 gctgttgtcc acttcaaggt gtcagcccag ggcattacac tgacggacaa ccaaaggaag
     5701 ctcttctttc gccgccatta tccagtgaac agcatcacct tctccagcac tgaccctcaa
     5761 gaccggagat ggaccaaccc agacgggacc acctccaaga tctttggttt cgtggccaag
     5821 aagccgggaa gcccctggga gaatgtgtgt cacctctttg cagagcttga cccagatcag
     5881 cctgctggcg ccattgtcac cttcatcacc aaagttctac tgggccagag aaaatgaatc
     5941 tagataactg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     6001 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     6061 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6121 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg
     6181 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     6241 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     6301 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     6361 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     6421 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     6481 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     6541 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     6601 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     6661 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6721 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
     6781 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     6841 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     6901 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     6961 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7021 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     7081 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
     7141 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     7201 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     7261 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     7321 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     7381 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     7441 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     7501 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     7561 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     7621 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     7681 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     7741 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     7801 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     7861 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     7921 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     7981 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     8041 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     8101 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
     8161 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     8221 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     8281 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     8341 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     8401 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8461 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8521 gttccactga gcgtcagacc ccgta
//
