LOCUS       sequence-204205-        9057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To study localization and function of Tensin 3 with EGFP Tensin1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9057)
  AUTHORS   Clark K, Howe JD, Pullar CE, Green JA, Artym VV, Yamada KM, 
            Critchley DR
  TITLE     Tensin 2 modulates cell contractility in 3D collagen gels through 
            the RhoGAP DLC1.
  JOURNAL   J Cell Biochem. 2010 Mar 1;109(4):808-17. doi: 10.1002/jcb.22460.
  PUBMED    20069572
REFERENCE   2  (bases 1 to 9057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/jcb"; 
            journalName: "J Cell Biochem"; date: "2010-03-1- 1"; volume: "109"; 
            issue: "4"; pages: "808-17"
COMMENT     Sequence Label: pmCherry Tensin3
FEATURES             Location/Qualifiers
     source          1..9057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      41..629
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     530..549
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        804..1107
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1108..1311
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1262..1282
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1350..1359
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1356..2063
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1501..1519)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1769..1788
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    6588..6709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6625..6644)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6679..6698
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6716..7171)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6853..6874)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7065..7084
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7198..7302
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7304..7661
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7304..7495
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7327..7347)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7512..7647
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7696..8490
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7696..8490
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7750..7769)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8360..8379
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8678..8697)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8722..8769
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
       61 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
      121 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
      181 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
      241 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
      301 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
      361 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
      421 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      481 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      541 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      601 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      661 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      721 ataaccgtat taccgccatg cattagttat taatagtaat caattacggg gtcattagtt
      781 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      841 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      901 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      961 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1021 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1081 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     1141 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     1201 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     1261 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
     1321 aaccgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     1381 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     1441 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     1501 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     1561 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     1621 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     1681 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     1741 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     1801 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     1861 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     1921 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     1981 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     2041 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcgaattcca
     2101 tggaggaggg ccatgggctg gacctcactt acatcacgga gcgcatcatc gctgtgtcct
     2161 tccctgccgg ctgctctgag gagtcctacc tgcacaacct acaggaggtc acgcgcatgc
     2221 tcaagtccaa gcacggggac aactacctgg tattaaacct ttcagaaaag agatatgacc
     2281 ttacgaagct taacccaaag atcatggatg tgggctggcc agagctccac gcaccgcccc
     2341 tggataagat gtgtaccata tgcaaggcgc aggagtcctg gctgaacagc aacctccagc
     2401 atgtggtcgt cattcactgc aggggcggga aaggacgcat aggagtggtc atatcatcct
     2461 acatgcattt caccaacgtc tcagccagcg ccgaccaggc ccttgacagg tttgcaatga
     2521 agaagtttta tgatgacaaa gtttcagctt taatgcagcc ttcccaaaaa cggtatgttc
     2581 agttcctcag tgggctcctg tccggatcgg tgaaaatgaa tgcctctccc ctgttcctgc
     2641 attttgtcat cctccacggc acccccaact tcgacacagg tggagtgtgc cggccctttc
     2701 tgaagctcta ccaagccatg cagcctgtgt acacctccgg gatctacaac gttggcccag
     2761 aaaaccccag caggatctgc atcgtcatcg agccggccca gcttctgaag ggagatgtca
     2821 tggtgaaatg ctaccacaag aaataccgct cggccacccg tgacgtcatt ttccgcctgc
     2881 agtttcacac tggggctgtg cagggctacg ggctggtgtt tgggaaggag gatctggaca
     2941 atgccagcaa agatgaccgt tttcctgact atgggaaggt tgaattagtc ttctctgcca
     3001 cgcctgagaa gattcaaggg tccgaacact tgtacaacga ccacggtgtg attgtggact
     3061 acaacacaac agacccactg atacgctggg actcgtacga gaacctcagt gcagatggag
     3121 aagtgctaca cacgcagggc cctgtcgatg gcagccttta cgcgaaggtg aggaagaaaa
     3181 gctcctcgga tcctggcatc ccaggtggcc cccaggcaat cccggccacc aacagcccag
     3241 accacagtga ccacaccttg tctgtcagca gtgactccgg ccactctaca gcctctgcca
     3301 ggacggataa gacggaagag cgcctggccc caggaaccag gaggggcctg agtgcccagg
     3361 agaaggctga gttggaccag ctgctcagtg gctttggcct ggaagatcct ggaagctccc
     3421 tcaaggaaat gactgatgct cgaagcaagt acagtgggac ccgccacgtg gtgccagccc
     3481 aggttcacgt gaatggagac gctgctctga aggatcggga gacagacatt ctggatgacg
     3541 agatgcccca ccacgacctg cacagtgtgg acagccttgg gaccctgtcc tcctcggaag
     3601 ggcctcagtc ggcccacctg ggtcccttca cctgccacaa gagcagccag aactcactcc
     3661 tatctgacgg ttttggcagc aacgttggtg aagatccgca gggcaccctc gttccggacc
     3721 tgggccttgg catggacggc ccctatgagc gggagcggac ttttgggagt cgagagccca
     3781 agcagcccca gcccctgctg agaaagccct cagtgtccgc ccagatgcag gcctatgggc
     3841 agagcagcta ctccacacag acctgggtgc gccagcagca gatggttgta gctcaccagt
     3901 atagcttcgc cccagatggg gaggcccggc tggtgagccg ctgccctgca gacaatcctg
     3961 gcctcgtcca ggcccagccc agagtgccac tcacccccac ccgagggacc agcagtaggg
     4021 tggctgtcca gaggggtgta ggcagtgggc cacatccccc tgacacacag cagccctctc
     4081 ccagcaaagc gttcaaaccc aggtttccag gagaccaggt tgtgaatgga gccggcccag
     4141 agctgagcac aggcccctcc ccaggctcgc ccaccctgga catcgaccag tccatcgagc
     4201 agctcaacag gctgatcctg gagctggatc ccaccttcga gcccatccct acccacatga
     4261 acgccctcgg tagccaggcc aatggctctg tgtctccaga cagcgtggga ggcgggctcc
     4321 gggcaagcag caggctgcct gacacaggag agggccccag cagggccacc gggcggcaag
     4381 gctcctctgc tgaacagccc ctgggcggga gactcaggaa gctgagcctg gggcagtacg
     4441 acaacgatgc tggggggcag ctgcccttct ccaaatgtgc atggggaaag gctggtgtgg
     4501 actatgcccc aaacctgccg ccattcccct caccagcgga cgtcaaagag acgatgaccc
     4561 ctggctatcc ccaggacctc gatattatcg atggcagaat tttaagtagc aaggagtcca
     4621 tgtgttcaac tccagcattt cctgtgtctc cagagacacc gtatgtgaaa acagcgctgc
     4681 gccatcctcc gttcagccca cctgagcccc cgctgagcag cccagccagt cagcacaaag
     4741 gaggacgtga accacgaagc tgccctgaga cgctcactca cgctgtgggg atgtcagaga
     4801 gccccatcgg acccaaatcc acgatgctcc gggctgatgc gtcctcgacg ccctcctttc
     4861 agcaggcttt tgcttcttcc tgcaccattt ccagcaacgg ccctgggcag aggagagaga
     4921 gctcctcttc tgcagaacgc cagtgggtgg agagcagccc caagcccatg gtttccctgc
     4981 tggggagcgg ccggcccacc ggaagtcccc tcagcgctga gttctccggt accaggaagg
     5041 actccccagt gctgtcctgc ttcccgccgt cagagctcca ggctcctttc cacagccatg
     5101 agctgtccct agcagagcca ccggactccc tggcgcctcc cagcagccag gccttcctgg
     5161 gcttcggcac cgccccagtg ggaagtggcc ttccgcccga ggaggacctg ggggccttgc
     5221 tggccaattc tcatggagcg tcaccgaccc ccagcatccc gctgacagcg acaggggctg
     5281 ccgacaatgg cttcctgtcc cacaactttc tcacggtggc gcctggacac agcagccacc
     5341 acagtccagg cctgcagggc cagggtgtga ccctgcccgg gcagccaccc ctccctgaga
     5401 agaagcgggc ctcggagggg gatcgttctt tgggctcagt ctctccctcc tccagtggct
     5461 tctccagccc gcacagcggg agcaccatca gtatcccctt cccaaatgtc cttcccgact
     5521 tttccaaggc ttcagaagcg gcctcacctc tgccagatag tccaggtgat aaacttgtga
     5581 tcgtgaaatt tgttcaagac acttccaagt tctggtacaa ggcggatatt tcaagagaac
     5641 aagccatcgc catgttgaag gacaaggagc cgggctcatt cattgttcga gacagccatt
     5701 ccttccgagg ggcctatggc ctggccatga aggtggccac gcccccacct tcagtcctgc
     5761 agctgaacaa gaaagctgga gatttggcca atgaactcgt ccggcacttt ttgatcgagt
     5821 gtaccccgaa gggagtgcgg ttgaaagggt gctcgaatga accatatttc gggagcctga
     5881 cggccttggt gtgccagcat tccatcacgc ccttggcctt gccgtacaag ctgcttatcc
     5941 cagagagaga tccattggag gaaatagcag aaagttctcc ccagacggca gccaattcag
     6001 cagctgagct gttgaagcag ggggcagcct gcaatgtgtg gtacttgaac tctgtggaga
     6061 tggagtccct caccggccac caggcgatcc agaaggccct gagcatcacc ctggtccagg
     6121 agcctccacc tgtgtccaca gttgtgcact tcaaggtgtc agcccagggc atcaccctga
     6181 cagacaatca gaggaagctc ttcttccgga ggcattaccc cgtgaacagt gtgattttct
     6241 gtgccttgga cccacaagac aggaagtgga tcaaagatgg cccttcctca aaagtctttg
     6301 gatttgtggc ccggaagcag ggcagtgcca cggataatgt gtgccacctg tttgcagagc
     6361 atgaccctga gcagcctgcc agtgccattg tcaacttcgt atcaaaggtc atgattggtt
     6421 caccaaagaa ggtctgagtc gacggtaccg cgggcccggg atccaccgga tctagataac
     6481 tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     6541 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     6601 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6661 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt
     6721 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     6781 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     6841 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     6901 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     6961 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     7021 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     7081 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     7141 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     7201 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7261 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
     7321 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7381 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     7441 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     7501 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7561 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7621 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
     7681 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     7741 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     7801 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     7861 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     7921 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     7981 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     8041 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     8101 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     8161 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     8221 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     8281 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     8341 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     8401 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     8461 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     8521 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     8581 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     8641 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac
     8701 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
     8761 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     8821 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     8881 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag
     8941 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9001 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttcc
//
