LOCUS       sequence-204207-        8517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8517)
  AUTHORS   Grefen C, Obrdlik P, Harter K
  TITLE     The determination of protein-protein interactions by the 
            mating-based split-ubiquitin system (mbSUS).
  JOURNAL   Methods Mol Biol. 2009;479:217-33. doi: 
            10.1007/978-1-59745-289-2_14.
  PUBMED    19083185
REFERENCE   2  (bases 1 to 8517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2009"; volume: "479:217-33. doi"; pages: " 
            10.1007/978-1-59745-289-2_14"
COMMENT     Sequence Label: pXNubA22-Dest
FEATURES             Location/Qualifiers
     source          1..8517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    368..492
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        666..768
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             769..1428
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(835..854)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1770..2075
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1954..1973
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2116..2240)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2386..2475
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      2508..2695
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2563..2587)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2722..2738)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2722..2738)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2735..2757)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2746..2762
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2770..2776,2777..2794,2795..2800))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2815..2836
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2953..2970)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3124..3712)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3204..3223)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3883..4674,4675..4743))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4506..4525
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4744..4848)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4875..6217
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(5838..5885)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     6258..6276
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6314..6336)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6436..6455
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6476..6757
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             6758..7432
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(7535..7990)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7672..7693)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7884..7903
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8116..8138
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8130..8147
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8131..8147
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(8170..8517,1..358)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 tgttgtctca ccatatccgc aatgacaaaa aaatgatgga agacactaaa ggaaaaaatt
       61 aacgacaaag acagcaccaa cagatgtcgt tgttccagag ctgatgaggg gtatctcgaa
      121 gcacacgaaa ctttttcctt ccttcattca cgcacactac tctctaatga gcaacggtat
      181 acggccttcc ttccagttac ttgaatttga aataaaaaaa agtttgctgt cttgctatca
      241 agtataaata gacctgcaat tattaatctt ttgtttcctc gtcattgttc tcgttccctt
      301 tcttccttgt ttctttttct gcacaatatt tcaagctata ccaagcatac aatcaactca
      361 gctgtgaaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa
      421 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
      481 ccagtcacta tggcggccgc gggtgatgct gccaacttag cggccgctaa gttggcagca
      541 tcacccgacg cactttgcgc cgaataaata cctgtgacgg aagatcactt cgcagaataa
      601 ataaatcctg gtgtccctgt tgataccggg aagccctggg ccaacttttg gcgaaaatga
      661 gacgttgatc ggcacgcaag aggttccaac tttcaccata atgaaataag atcactaccg
      721 ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
      781 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
      841 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
      901 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
      961 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
     1021 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     1081 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     1141 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     1201 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     1261 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     1321 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
     1381 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaaac gcgtggatcc
     1441 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
     1501 atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta
     1561 ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat
     1621 ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg
     1681 gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc
     1741 ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag
     1801 agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac
     1861 ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt
     1921 acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg
     1981 tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca
     2041 aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc
     2101 agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
     2161 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
     2221 gctttcttgt acaaagtggt tggtggtggc ggatccggtg gaggtggatc aatgcagatt
     2281 ttcgtcaaga ctttgaccgg taaaaccgca acgttggaag ttgaatcttc cgataccatc
     2341 gacaacgtta agtcgaaaat tcaagacaag gaaggaatcc ctggttaccc atacgatgtt
     2401 cctgactatg cgggctatcc ctatgacgtc ccggactatg caggatccta tccatatgac
     2461 gttccagatt acgctgctca gtgctgaggc gcgccacttc taaataagcg aatttcttat
     2521 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
     2581 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca
     2641 ggttgctttc tcaggtatag tatgaggtcg ctcttattga ccacacctct accggcagca
     2701 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     2761 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     2821 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     2881 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     2941 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3001 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3061 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3121 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3181 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3241 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3301 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3361 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3421 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3481 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3541 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3601 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3661 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3721 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3781 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3841 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3901 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3961 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4021 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4081 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4141 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4201 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4261 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4321 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4381 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4441 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4501 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4561 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4621 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4681 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4741 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4801 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4861 aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga
     4921 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg
     4981 cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa
     5041 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
     5101 caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa
     5161 tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg
     5221 tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa
     5281 gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg
     5341 tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta
     5401 tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat
     5461 tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat
     5521 aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa
     5581 tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag
     5641 atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat
     5701 atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt
     5761 agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg
     5821 gttttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga
     5881 acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac
     5941 agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga
     6001 agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg
     6061 atgaaaggta gtctagtacc tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc
     6121 ttccttcagc actacccttt agctgttcta tatgctgcca ctcctcaatt ggattagtct
     6181 catccttcaa tgctatcatt tcctttgata ttggatcata ctaagaaacc attattatca
     6241 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     6301 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     6361 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     6421 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat agatcaacga
     6481 cattactata tatataatat aggaagcatt taatagaaca gcatcgtaat atatgtgtac
     6541 tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa aaatagcttg
     6601 tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa gaattaattc
     6661 ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat tgagcacgtg
     6721 agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta atttcacagg
     6781 tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg cagaatgtgc
     6841 tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa agagaacaat
     6901 tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa atagttcagg
     6961 cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg ttttggctct
     7021 ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt ggcaagaata
     7081 ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag actgcaacat
     7141 actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg attcagaagc
     7201 aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg gaaggcaaga
     7261 gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa atgttggtga
     7321 tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg
     7381 tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat aggttattac
     7441 tgagtagtat ttatttaagt attgtttgtg cacttgccga tctatgcggt gtgaaatacc
     7501 gcacagatgc gtaaggagaa aataccgcat caggaaattg taagcgttaa tattttgtta
     7561 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     7621 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     7681 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     7741 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     7801 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     7861 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     7921 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     7981 cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     8041 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     8101 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctttcgc
     8161 gagctcgata tccttttgtt gtttccgggt gtacaatatg gacttcctct tttctggcaa
     8221 ccaaacccat acatcgggat tcctataata ccttcgttgg tctccctaac atgtaggtgg
     8281 cggaggggag atatacaata gaacagatac cagacaagac ataatgggct aaacaagact
     8341 acaccaatta cactgcctca ttgatggtgg tacataacga actaatactg tagccctaga
     8401 cttgatagcc atcatcatat cgaagtttca ctaccctttt tccatttgcc atctattgaa
     8461 gtaataatag gcgcatgcaa cttcttttct tttttttttc ttttctctct cccccgt
//
