LOCUS       sequence-204219-        9767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Cre recombinase reporter; 
            EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked 
            RFP-STOP cassette and switches fluorescence from red (RFP) to green 
            (GFP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR, Clevers H, Prasad V,
            Lebbink RJ, Rehmann H, Geijsen N
  TITLE     Efficient Intracellular Delivery of Native Proteins.
  JOURNAL   Cell. 2015 Apr 23;161(3):674-690. doi: 10.1016/j.cell.2015.03.028.
  PUBMED    25910214
REFERENCE   2  (bases 1 to 9767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.03"; journalName: "Cell"; date: "2015-04-23- 
            23"; volume: "161"; issue: "3"; pages: "674-690"
COMMENT     Sequence Label: Cre Reporter
FEATURES             Location/Qualifiers
     source          1..9767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     372..392
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    complement(455..488)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      517..526
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             523..1200
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(703..723)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     921..940
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1115..1138
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(1225..1258)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      1309..1318
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1315..1317,1318..1320,1321..2034)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1315..1317,1318..1320,1321..2031)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1360..1381)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1621..1640)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1968..1989
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2038..2605
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2055..2607
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2199..2216)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2426..2445
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2619..3218
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2619..2638)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3115..3135
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3232..3820
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3285..3305)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3703..3714)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3968..4148
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4195..4213)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4281..4385
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4386..4454,4455..5246)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4604..4623)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5417..6005
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6159..6176
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6416..6437
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6452..6457,6458..6475,6476..6482)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6490..6506
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6495..6517
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6514..6530
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6514..6530
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6580..6599
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6791..7424
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7471..7596
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8093..8326
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8511..8555
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8848..8965
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(9013..9767,1..424)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(9244..9767,1..415)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
ORIGIN
        1 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
       61 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
      121 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
      181 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
      241 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
      301 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
      361 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
      421 gtgaagcggc cgcctagtcc agtgtggtgg aattataact tcgtatagca tacattatac
      481 gaagttataa ttctgcagat atccagcaca gtggcggcca ccatggatag cactgagaac
      541 gtcatcaagc ccttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
      601 ttcgagatcg agggcgaggg cgagggcaag ccctacgagg gcacccagac cgccaagctg
      661 caggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttccag
      721 tacggctcca aggtgtacgt gaagcacccc gccgacatcc ccgactacaa gaagctgtcc
      781 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
      841 gtgacccagg actcctccct gcaggacggc accttcatct accacgtgaa gttcatcggc
      901 gtgaacttcc cctccgacgg ccccgtaatg cagaagaaga ctctgggctg ggagccctcc
      961 accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacaa ggcgctgaag
     1021 ctgaagggcg gcggccacta cctggtggag ttcaagtcaa tctacatggc caagaagccc
     1081 gtgaagctgc ccggctacta ctacgtggac tccaagctgg acatcacctc ccacaacgag
     1141 gactacaccg tggtggagca gtacgagcgc gccgaggccc gccaccacct gttccagtag
     1201 agctagctga ataaggccgc tcgaataact tcgtatagca tacattatac gaagttattc
     1261 gagtctagag ggcccgttag atctctcgag gttaacgaat tcgactacgc caccatggtg
     1321 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1381 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     1441 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1501 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     1561 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1621 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1681 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1741 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     1801 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     1861 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1921 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1981 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaggatcc
     2041 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     2101 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     2161 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     2221 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     2281 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     2341 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     2401 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     2461 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     2521 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     2581 tttcctttga aaaacacgat gataatatgg ccacacatat gaccgagtac aagcccacgg
     2641 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     2701 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     2761 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     2821 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     2881 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     2941 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     3001 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     3061 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     3121 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     3181 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaat cgatagatcc taatcaacct
     3241 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3301 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     3361 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     3421 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     3481 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     3541 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     3601 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     3661 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     3721 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     3781 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat
     3841 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     3901 gctaattcac tcccaacgaa gacaagatca cctgcaggac aggcgcgccc tgctttttgc
     3961 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     4021 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     4081 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     4141 ctctagcacc cgggcgatta aggaaagggc tagatcattc ttgaagacga aagggcctcg
     4201 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     4261 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4321 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4381 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     4441 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     4501 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     4561 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     4621 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     4681 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     4741 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     4801 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     4861 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4921 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     4981 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5041 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5101 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5161 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5221 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5281 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5341 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5401 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5461 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5521 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5581 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5641 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5701 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5761 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5821 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5881 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5941 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6001 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6061 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     6121 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     6181 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     6241 gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     6301 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     6361 ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     6421 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     6481 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga
     6541 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6601 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
     6661 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     6721 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     6781 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
     6841 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     6901 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     6961 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     7021 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     7081 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     7141 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     7201 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     7261 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7321 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7381 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     7441 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     7501 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     7561 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     7621 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     7681 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     7741 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     7801 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     7861 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     7921 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     7981 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     8041 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     8101 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     8161 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     8221 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     8281 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     8341 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     8401 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     8461 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     8521 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     8581 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     8641 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     8701 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     8761 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa
     8821 ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     8881 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     8941 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     9001 gactagtcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     9061 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     9121 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     9181 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     9241 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     9301 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
     9361 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
     9421 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
     9481 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     9541 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
     9601 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     9661 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     9721 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggc
//
