LOCUS       sequence-20423-e         620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG424GPD-ccdB
FEATURES             Location/Qualifiers
     source          1..620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(99..223)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(256..272)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(257..273)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      275..522
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(291..309)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(540..559)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(541..559)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(569..586)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(569..585)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(578..600)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat
       61 gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg
      121 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
      181 tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtgatgggc tgcaggaatt
      241 cgatatcaag cttatcgata ccgtcgacct cgagtcatgt aattagttat gtcacgctta
      301 cattcacgcc ctccccccac atccgctcta accgaaaagg aaggagttag acaacctgaa
      361 gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt
      421 caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc
      481 ttgcttgaga aggttttggg acgctcgaag gctttaattt gcggccggta cccaattcgc
      541 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
      601 aaaaccctgg cgttacccaa
//
