LOCUS       sequence-204233-       11536 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent tagging in stably transformed plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11536)
  AUTHORS   Blatt MR, Grefen C
  TITLE     Applications of fluorescent marker proteins in plant cell biology.
  JOURNAL   Methods Mol Biol. 2014;1062:487-507. doi: 
            10.1007/978-1-62703-580-4_26.
  PUBMED    24057383
REFERENCE   2  (bases 1 to 11536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2014"; volume: "1062:487-507. doi"; pages: " 
            10.1007/978-1-62703-580-4_26"
COMMENT     Sequence Label: pUBC-paGFP-Dest
FEATURES             Location/Qualifiers
     source          1..11536
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..793
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(818..823,824..841,842..848)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             902..1561
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(968..987)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1903..2208
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2087..2106
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2249..2373)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(2426..2428,2429..2431,2432..3148)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKL"
     primer_bind     complement(2471..2492)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2732..2751)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3079..3100
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        3512..3691
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             3733..4284
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      4304..4556
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4649..4673
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5194..5985
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6231..6819
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6720..6739
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6973..6990
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7005..7145
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7071..7090)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7231..7253
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7489..7683
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7749..8822)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9251..9880)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11211..11235
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11400..11421
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11436..11441,11442..11459,11460..11466)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11474..11490
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11479..11501
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11498..11514
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11498..11514
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca
      721 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
      781 tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc
      841 gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa
      901 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
      961 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     1021 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     1081 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
     1141 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     1201 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     1261 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     1321 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     1381 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     1441 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     1501 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     1561 aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
     1621 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct
     1681 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
     1741 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
     1801 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
     1861 aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac
     1921 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     1981 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     2041 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     2101 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
     2161 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
     2221 cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta
     2281 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
     2341 cgtttctcgt tcagctttct tgtacaaagt ggtgatggga cgtccgcgga gatctacgcg
     2401 tgtcgactcg agatatccaa ctagtatggt gagcaagggc gaggagctgt tcaccggggt
     2461 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     2521 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     2581 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     2641 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     2701 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     2761 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     2821 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     2881 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     2941 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3001 ccccgtgctg ctgcccgaca accactactt aagccaccag tccgccctga gcaaagaccc
     3061 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     3121 cggcatggac gagctgtaca agttataagc ggccatgcta gagtccgcaa aaatcaccag
     3181 tctctctcta caaatctatc tctctctatt tttctccaga ataatgtgtg agtagttccc
     3241 agataaggga attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc
     3301 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
     3361 aaaatccagt gacctgcagg catgcgacgt cgggccctct agaggatccc cgggtaccgc
     3421 gaattatcga tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc
     3481 gggacaagcc gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactgagc
     3541 cgcgggtttc tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc
     3601 aaaagtcgcc taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga
     3661 cgttccataa attcccctcg gtatccaatt agagtctcat attcactctc aactcgatcg
     3721 aggggatcta ccatgagccc agaacgacgc ccggccgaca tccgccgtgc caccgaggcg
     3781 gacatgccgg cggtctgcac catcgtcaac cactacatcg agacaagcac ggtcaacttc
     3841 cgtaccgagc cgcaggaacc gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc
     3901 tatccctggc tcgtcgccga ggtggacggc gaggtcgccg gcatcgccta cgcgggtccc
     3961 tggaaggcac gcaacgccta cgactggacg gccgagtcga ccgtgtacgt ctccccccgc
     4021 caccagcgga cgggactggg ctccacgctc tacacccacc tgctgaagtc cctggaggca
     4081 cagggcttca agagcgtggt cgctgtcatc gggctgccca acgacccgag cgtgcgcatg
     4141 cacgaggcgc tcggatatgc cccccgcggc atgctgcggg cggccggctt caagcacggg
     4201 aactggcatg acgtgggttt ctggcagctg gacttcagcc tgccggtgcc gccccgtccg
     4261 gtcctgcccg tcaccgaaat ctgatgaccc ctagagtcaa gcagatcgtt caaacatttg
     4321 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4381 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4441 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4501 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgacc
     4561 ggcatgcaag ctgataattc aattcggcgt taattcagta cattaaaaac gtccgcaatg
     4621 tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca
     4681 gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat
     4741 cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac
     4801 cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg
     4861 gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aattcgggca
     4921 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     4981 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     5041 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     5101 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     5161 cagggcagtc gccctaaaac aaagttaaac atcatggggg aagcggtgat cgccgaagta
     5221 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
     5281 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat
     5341 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
     5401 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
     5461 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
     5521 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
     5581 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
     5641 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
     5701 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     5761 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
     5821 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     5881 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     5941 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct agctagaaat
     6001 tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca
     6061 cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca
     6121 taataagccc tacacaaatt gggagatata tcatgcatga ccaaaatccc ttaacgtgag
     6181 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6241 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6301 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6361 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     6421 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6481 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6541 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6601 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6661 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6721 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6781 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6841 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6901 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6961 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     7021 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     7081 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     7141 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     7201 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     7261 cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc
     7321 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
     7381 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
     7441 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
     7501 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
     7561 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
     7621 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
     7681 acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
     7741 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
     7801 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
     7861 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
     7921 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
     7981 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
     8041 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
     8101 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
     8161 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
     8221 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
     8281 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
     8341 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
     8401 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
     8461 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
     8521 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
     8581 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct
     8641 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
     8701 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
     8761 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
     8821 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc
     8881 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
     8941 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
     9001 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
     9061 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
     9121 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
     9181 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
     9241 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
     9301 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
     9361 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
     9421 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
     9481 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
     9541 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
     9601 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
     9661 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
     9721 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
     9781 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
     9841 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
     9901 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
     9961 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
    10021 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac
    10081 gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt
    10141 aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt
    10201 tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg
    10261 cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc
    10321 gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc
    10381 agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg
    10441 cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg
    10501 aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg
    10561 gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg
    10621 tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg
    10681 ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg
    10741 atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc
    10801 aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg
    10861 ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac
    10921 gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg
    10981 tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag
    11041 cagcggaggg gttggatcaa agtactttaa agtactttaa agtactttaa agtactttga
    11101 tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt
    11161 cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc
    11221 caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac aatctgatcc
    11281 aagctcaagc tgctctagcc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    11341 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
    11401 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
    11461 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt
    11521 acgaattcga gctcgg
//
