LOCUS       sequence-204235-       11493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent tagging in stably transformed plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11493)
  AUTHORS   Blatt MR, Grefen C
  TITLE     Applications of fluorescent marker proteins in plant cell biology.
  JOURNAL   Methods Mol Biol. 2014;1062:487-507. doi: 
            10.1007/978-1-62703-580-4_26.
  PUBMED    24057383
REFERENCE   2  (bases 1 to 11493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2014"; volume: "1062:487-507. doi"; pages: " 
            10.1007/978-1-62703-580-4_26"
COMMENT     Sequence Label: pUBN-paGFP-Dest
FEATURES             Location/Qualifiers
     source          1..11493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(666..668,669..671,672..1382)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(711..732)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(972..991)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1319..1340
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1395..1519
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1544..1549,1550..1567,1568..1574)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1628..2287
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1694..1713)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2629..2934
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2813..2832
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2975..3099)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3469..3648
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             3690..4241
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      4261..4513
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4606..4630
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5151..5942
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6188..6776
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6677..6696
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6930..6947
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6962..7102
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7028..7047)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7188..7210
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7446..7640
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7706..8779)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9208..9837)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11168..11192
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11357..11378
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11393..11398,11399..11416,11417..11423)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11431..11447
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11436..11458
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11455..11471
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11455..11471
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagaa
      661 ctagtatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
      721 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
      781 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
      841 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
      901 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
      961 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     1021 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     1081 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     1141 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     1201 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1261 accactactt aagccaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     1321 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     1381 agcaattgtt aatcacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa
     1441 atatcaatat attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac
     1501 aacatatcca gtcactatgg cggccgcatt aggcacccca ggctttacac tttatgcttc
     1561 cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga
     1621 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
     1681 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
     1741 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
     1801 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
     1861 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
     1921 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
     1981 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
     2041 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
     2101 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
     2161 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg
     2221 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
     2281 ggcgtaaacg cgtggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc
     2341 tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg
     2401 tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag
     2461 gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc
     2521 gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt
     2581 ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag
     2641 gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt
     2701 attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat
     2761 aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg
     2821 accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc
     2881 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca
     2941 ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt
     3001 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
     3061 attttacgtt tctcgttcag ctttcttgta caaagtggtg attaagcggc catgctagag
     3121 tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt ctccagaata
     3181 atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg ctcatgtgtt
     3241 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
     3301 ttctaattcc taaaaccaaa atccagtgac ctgcaggcat gcgacgtcgg gccctctaga
     3361 ggatccccgg gtaccgcgaa ttatcgatca tgagcggaga attaagggag tcacgttatg
     3421 acccccgccg atgacgcggg acaagccgtt ttacgtttgg aactgacaga accgcaacgt
     3481 tgaaggagcc actgagccgc gggtttctgg agtttaatga gctaagcaca tacgtcagaa
     3541 accattattg cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa tatttcttgt
     3601 caaaaatgct ccactgacgt tccataaatt cccctcggta tccaattaga gtctcatatt
     3661 cactctcaac tcgatcgagg ggatctacca tgagcccaga acgacgcccg gccgacatcc
     3721 gccgtgccac cgaggcggac atgccggcgg tctgcaccat cgtcaaccac tacatcgaga
     3781 caagcacggt caacttccgt accgagccgc aggaaccgca ggagtggacg gacgacctcg
     3841 tccgtctgcg ggagcgctat ccctggctcg tcgccgaggt ggacggcgag gtcgccggca
     3901 tcgcctacgc gggtccctgg aaggcacgca acgcctacga ctggacggcc gagtcgaccg
     3961 tgtacgtctc cccccgccac cagcggacgg gactgggctc cacgctctac acccacctgc
     4021 tgaagtccct ggaggcacag ggcttcaaga gcgtggtcgc tgtcatcggg ctgcccaacg
     4081 acccgagcgt gcgcatgcac gaggcgctcg gatatgcccc ccgcggcatg ctgcgggcgg
     4141 ccggcttcaa gcacgggaac tggcatgacg tgggtttctg gcagctggac ttcagcctgc
     4201 cggtgccgcc ccgtccggtc ctgcccgtca ccgaaatctg atgaccccta gagtcaagca
     4261 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     4321 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     4381 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     4441 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     4501 atgttactag atcgaccggc atgcaagctg ataattcaat tcggcgttaa ttcagtacat
     4561 taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata
     4621 tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact
     4681 cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca
     4741 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
     4801 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
     4861 tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     4921 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     4981 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     5041 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     5101 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag
     5161 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     5221 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     5281 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     5341 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     5401 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     5461 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     5521 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     5581 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     5641 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     5701 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     5761 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     5821 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     5881 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     5941 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     6001 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     6061 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca
     6121 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6181 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6241 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6301 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     6361 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6421 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6481 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6541 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6601 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6661 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6721 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6781 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6841 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6901 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     6961 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     7021 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     7081 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     7141 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     7201 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc
     7261 gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggcccta
     7321 ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta
     7381 gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag
     7441 acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg
     7501 cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa
     7561 tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc
     7621 caatgtacgt gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa
     7681 tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt
     7741 tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact
     7801 ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc
     7861 cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg
     7921 cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat
     7981 cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat
     8041 cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc
     8101 ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg
     8161 tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt
     8221 tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga
     8281 acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat
     8341 gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg
     8401 cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt
     8461 cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca
     8521 cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa
     8581 tcgacggcgc accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg
     8641 cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg
     8701 cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg
     8761 atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg
     8821 ccggcagaca acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca
     8881 ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa
     8941 aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag
     9001 atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat
     9061 cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca
     9121 acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt
     9181 tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag
     9241 cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc
     9301 ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc
     9361 gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga
     9421 cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc
     9481 caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa
     9541 gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac
     9601 tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc
     9661 gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg
     9721 atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt
     9781 tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg
     9841 ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc
     9901 aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg
     9961 gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc
    10021 agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc
    10081 gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg
    10141 cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt
    10201 cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa
    10261 gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc
    10321 ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc
    10381 gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga
    10441 tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg
    10501 ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct
    10561 ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg
    10621 tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt
    10681 cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt
    10741 cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc
    10801 cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact
    10861 ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc
    10921 gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga
    10981 agccgactgc actatagcag cggaggggtt ggatcaaagt actttaaagt actttaaagt
    11041 actttaaagt actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac
    11101 gaacggataa accttttcac gcccttttaa atatccgatt attctaataa acgctctttt
    11161 ctcttaggtt tacccgccaa tatatcctgt caaacactga tagtttaaac tgaaggcggg
    11221 aaacgacaat ctgatccaag ctcaagctgc tctagccaat acgcaaaccg cctctccccg
    11281 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    11341 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    11401 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    11461 acagctatga catgattacg aattcgagct cgg
//
