LOCUS       sequence-204238-       13305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET/FLIM analysis in plants (stable or transient transformation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13305)
  AUTHORS   Hecker A, Wallmeroth N, Peter S, Blatt MR, Harter K, Grefen C
  TITLE     Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic 
            Protein Interaction Studies.
  JOURNAL   Plant Physiol. 2015 Jul;168(3):776-87. doi: 10.1104/pp.15.00533. 
            Epub 2015 May 13.
  PUBMED    25971551
REFERENCE   2  (bases 1 to 13305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.15.00533"; journalName: "Plant Physiol"; date: 
            "2015-07"; volume: "168"; issue: "3"; pages: "776-87"
COMMENT     Sequence Label: pFRETvr-2in1-CN
FEATURES             Location/Qualifiers
     source          1..13305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        143..487
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1304..1428
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1453..1458,1459..1476,1477..1483)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1537..2196
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1603..1622)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2538..2843
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2722..2741
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2884..3008)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3034..3063
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3064..3093
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        3433..3777
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    3872..3995
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4030..4035,4036..4053,4054..4060)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4068..4084
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4073..4095
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4092..4108
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4092..4108
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(4369..4493)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(4513..4515,4516..4518,4519..5232)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4558..4579)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4819..4838)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5166..5187
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5557..5735
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      6348..6600
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6705..6729
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7250..8041
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8287..8875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8776..8795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9029..9046
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9061..9201
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9127..9146)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9287..9309
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9545..9739
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9805..10878)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11307..11936)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13267..13291
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aaactgaagg cgggaaacga caatctgatc caagctcaag ctaagctagg actagccctc
       61 tgcaggtcaa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag
      121 tctcagaaga ccagagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc
      181 tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaagatg
      241 gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctaccg
      301 acagtggtcc caaagatgga cccccaccca cgaggaacat cgtggaaaaa gaagacgttc
      361 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
      421 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
      481 agaggacctc gagcgcgcgt atttttaaac aattaccaac aacaacaaca aacaacaaca
      541 acattacatt ttacattcta caactacatc tagtatggtg agtaaaggtg aagagttgat
      601 taaagagaac atgcatatga agttatacat ggagggaact gtcaataatc accactttaa
      661 gtgtacatca gagggtgagg gaaagccata cgagggaacc caaactatga gaatcaaagt
      721 agtggaggga ggtcctcttc catttgcttt tgatatacta gcaacaagtt tcatgtatgg
      781 ttccaggacc ttcattaacc atactcaggg aatccctgac ttctttaaac agtcttttcc
      841 tgaaggtttt acatgggaga gggttaccac ttacgaggac ggtggagtct tgacagcaac
      901 ccaggacact tcattacaag atggatgcct aatatacaat gtgaaaatta ggggtgtgaa
      961 tttccctagt aacggaccag ttatgcagaa gaaaacacta ggttgggaag ctaatactga
     1021 aatgttgtac cctgccgacg gaggtttaga aggtagatcc gacatggccc ttaagctagt
     1081 cggaggtgga cacttgatct gtaactttaa aaccacatat aggtctaaga agccagcaaa
     1141 gaatctaaaa atgcctggtg tttactatgt ggaccataga ctagaaagga taaaagaagc
     1201 agacaaagaa acttacgtgg agcaacatga ggtcgccgtc gctaggtatt gcgacttacc
     1261 ttccaagcta ggtcacaaat tgaacagtaa cactagtcct agaacaagtt tgtacaaaaa
     1321 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     1381 aaaacagact acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta
     1441 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     1501 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1561 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1621 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1681 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1741 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     1801 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     1861 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     1921 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     1981 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     2041 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     2101 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2161 caacagtact gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg cttactaaaa
     2221 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     2281 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
     2341 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     2401 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     2461 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     2521 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     2581 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     2641 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     2701 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     2761 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     2821 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     2881 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     2941 atctaattta atatattgat atttatatca ttttacgttt ctcgttcaac ttttctatac
     3001 aaagttggtc aattgttagg cggtggatct ctcgagcaga agttgatctc agaggaggac
     3061 ctcgagcaga agttgatctc agaggaggac ttataagcgg ccatgctaga gtccgcaaaa
     3121 atcaccagtc tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag
     3181 tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata
     3241 agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc
     3301 ctaaaaccaa aatccagtga aggctcgcac gtgcggagct ccgtaccctc tgcaggtcaa
     3361 catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga
     3421 ccagagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca
     3481 ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaagatg gcttctacaa
     3541 atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctaccg acagtggtcc
     3601 caaagatgga cccccaccca cgaggaacat cgtggaaaaa gaagacgttc caaccacgtc
     3661 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
     3721 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agaggacctc
     3781 gagcgcgcgt atttttaaac aattaccaac aacaacaaca aacaacaaca acattacatt
     3841 ttacattcta caactacatc tagtcaattg gcaactttgt ataataaagt tgaacgagaa
     3901 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
     3961 taatactgta aaacacaaca tatccagtca ctatggcggc cgcgggctca ctcattaggc
     4021 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     4081 acaatttcac acaggaaaca gctatgacca tgattacccc gtccctgcac gcctgtcgtt
     4141 caaccctgga agatccgcgt gtcccgagtt ctaacagtct ggccgtcgtt ctgcagcgtc
     4201 gcgattggga aaacccgggc gtgacccaac tgaatcgtct ggcggcccat ccgccgtttg
     4261 cgtcgtggcg taactcagaa gaagcccgta ccgaccgtcc gtctcaacaa ctgcgctcct
     4321 aacactataa aaagctgatg cagacggggc ttttttctgc aggtcgacca tagtgactgg
     4381 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     4441 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtgtctagg
     4501 ttaggcacta gtatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     4561 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     4621 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg
     4681 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc
     4741 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     4801 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     4861 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     4921 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
     4981 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
     5041 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     5101 cccgacaacc actacctgag ctaccagtcc aagctgagca aggatcccaa cgagaagcgc
     5161 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     5221 ctgtacaagt aattaaagcg gccatgctag agtccgcaaa aatcaccagt ctctctctac
     5281 aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa
     5341 ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat
     5401 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg
     5461 acctaggagg gtagatcatg agcggagaat taagggagtc acgttatgac ccccgccgat
     5521 gacgcgggac aagccgtttt acgtttggaa ctgacagaac cgcaacgttg aaggagccac
     5581 tgagcgcggg tttctggagt ttaatgagct aagcacatac gtcagaaacc attattgcgc
     5641 gttcaaaagt cgcctaaggt cactatcatc tagcaaatat ttcttgtcaa aaatgctcca
     5701 ctgacgttcc ataaattccc ctcggtatcc aattagagtc tcatattcac tctcaactcg
     5761 atcgagggga tctaccatga gtccagaaag gagaccagca gatattagga gagcaaccga
     5821 agcagatatg ccagcagttt gcaccattgt gaaccattac atcgagactt ctacagttaa
     5881 ttttaggact gaacctcaag agccacagga atggacagat gatctcgtga gattaaggga
     5941 aagataccct tggcttgttg ctgaggtgga tggagaagtt gcaggtattg cttacgcagg
     6001 accttggaag gctagaaacg cttatgattg gactgctgag tctaccgttt acgtgtcacc
     6061 tagacatcaa agaaccggac ttggttcaac cttgtatact caccttttga agtctcttga
     6121 agctcaggga ttcaaatctg ttgtggctgt tatcggtttg cctaatgatc caagtgtgag
     6181 aatgcatgaa gctctcggat acgcaccaag gggtatgtta agagctgctg gatttaaaca
     6241 tggtaactgg cacgatgttg gtttctggca gttagatttc agtttaccag ttccaccaag
     6301 accagtgctt ccagtgaccg agatttgacc cgggctagag tcaagcagat cgttcaaaca
     6361 tttggcaata aagtttctta atattgaatc ctgttgccgg tcttgcgatg attatcatat
     6421 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
     6481 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
     6541 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
     6601 gaccggcatg aagctgagtt aacgatgtaa gtcctcaatt cggcgttaat tcagtacatt
     6661 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
     6721 atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc
     6781 gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag
     6841 actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa
     6901 cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct
     6961 gtgatcaatt cgggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     7021 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     7081 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     7141 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     7201 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc
     7261 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     7321 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     7381 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     7441 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     7501 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     7561 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     7621 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     7681 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     7741 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     7801 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     7861 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     7921 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     7981 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     8041 atgtctagct agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg
     8101 ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat
     8161 tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa
     8221 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8281 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8341 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8401 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     8461 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8521 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8581 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8641 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8701 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8761 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8821 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8881 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8941 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     9001 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     9061 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     9121 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     9181 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     9241 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     9301 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg
     9361 ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccctag
     9421 gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag
     9481 ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga
     9541 cagttatgca caggccaggc gggttttaag agttttaata agttttaaag agttttaggc
     9601 ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat
     9661 gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc
     9721 aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat
     9781 ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt
     9841 gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc
     9901 cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc
     9961 ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc
    10021 ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc
    10081 ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc
    10141 agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg
    10201 gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt
    10261 acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt
    10321 ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa
    10381 cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg
    10441 ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc
    10501 ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc
    10561 acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac
    10621 gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat
    10681 cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc
    10741 ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc
    10801 ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga
    10861 tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc
    10921 cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat
    10981 tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa
    11041 attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga
    11101 tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc
    11161 ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa
    11221 cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt
    11281 gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc
    11341 gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg
    11401 gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg
    11461 tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac
    11521 acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc
    11581 accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag
    11641 tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact
    11701 acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg
    11761 acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga
    11821 tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt
    11881 gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt
    11941 tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca
    12001 agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg
    12061 ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca
    12121 gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg
    12181 cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc
    12241 ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc
    12301 ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag
    12361 tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg
    12421 gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg
    12481 ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat
    12541 gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc
    12601 cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg
    12661 gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt
    12721 cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc
    12781 gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc
    12841 ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc
    12901 agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt
    12961 tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg
    13021 cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa
    13081 gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttaaagta ctttaaagta
    13141 ctttaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg
    13201 aacggataaa ccttttcacg cccttttaaa tatccgatta ttctaataaa cgctcttttc
    13261 tcttaggttt acccgccaat atatcctgtc aaacactgat agttt
//
