LOCUS       sequence-204246-       13389 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET/FLIM analysis in plants (stable or transient transformation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13389)
  AUTHORS   Hecker A, Wallmeroth N, Peter S, Blatt MR, Harter K, Grefen C
  TITLE     Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic 
            Protein Interaction Studies.
  JOURNAL   Plant Physiol. 2015 Jul;168(3):776-87. doi: 10.1104/pp.15.00533. 
            Epub 2015 May 13.
  PUBMED    25971551
REFERENCE   2  (bases 1 to 13389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.15.00533"; journalName: "Plant Physiol"; date: 
            "2015-07"; volume: "168"; issue: "3"; pages: "776-87"
COMMENT     Sequence Label: pFRETgc-2in1-NC
FEATURES             Location/Qualifiers
     source          1..13389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        165..509
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    603..727
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(752..757,758..775,776..782)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             836..1495
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(902..921)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1837..2142
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2021..2040
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2183..2307)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2327..3037
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2740..2759
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        3426..3770
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(3858..3860,3861..3863,3864..4574)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3903..3924)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4164..4183)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4511..4532
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    4594..4717
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4752..4757,4758..4775,4776..4782)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4790..4806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4795..4817
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4814..4830
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4814..4830
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(5091..5215)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5232..5258
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5262..5288
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5298..5324
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5641..5819
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      6432..6684
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6789..6813
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7334..8125
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8371..8959
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8860..8879
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9113..9130
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9145..9285
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9211..9230)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9371..9393
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9629..9823
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9889..10962)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11391..12020)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13351..13375
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aaactgaagg cgggaaacga caatctgatc caagctcaag ctaagctagg actagcaacg
       61 acggccagtg aattcaggcc tctgcaggtc aacatggtgg agcacgacac tctcgtctac
      121 tccaagaata tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa
      181 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa
      241 aggacagtag aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct
      301 atcgttcaag atgcctctac cgacagtggt cccaaagatg gacccccacc cacgaggaac
      361 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
      421 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
      481 taaggaagtt catttcattt ggagaggacc tcgagcgcgc gtatttttaa acaattacca
      541 acaacaacaa caaacaacaa caacattaca ttttacattc tacaactaca tctagtccta
      601 gaacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat
      661 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt
      721 cactatggcg gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa
      781 tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga
      841 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
      901 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
      961 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
     1021 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct
     1081 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
     1141 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
     1201 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
     1261 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
     1321 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
     1381 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
     1441 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaagatc
     1501 tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
     1561 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag
     1621 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg
     1681 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcttgccg
     1741 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga
     1801 acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat
     1861 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc
     1921 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
     1981 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     2041 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     2101 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
     2161 cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt
     2221 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
     2281 tcgttcaact tttctataca aagttggtca attgttaggc actagtatgg tgagcaaggg
     2341 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     2401 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     2461 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     2521 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     2581 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     2641 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     2701 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     2761 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     2821 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     2881 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     2941 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     3001 ccactccacc ggcggcatgg acgagctgta caagtaatta taagcggcca tgctagagtc
     3061 cgcaaaaatc accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat
     3121 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
     3181 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     3241 ctaattccta aaaccaaaat ccagtgacct aggaggcctg catgcaagct tggcgtaatc
     3301 atggtcatag ctgttgctcg cacgtgcgga gctccgtacc ctctgcaggt caacatggtg
     3361 gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccagagg
     3421 gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca
     3481 gctatctgtc acttcatcga aaggacagta gaaaaggaag atggcttcta caaatgccat
     3541 cattgcgata aaggaaaggc tatcgttcaa gatgcctcta ccgacagtgg tcccaaagat
     3601 ggacccccac ccacgaggaa catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
     3661 caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct
     3721 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac ctcgagcgcg
     3781 cgtattttta aacaattacc aacaacaaca acaaacaaca acaacattac attttacatt
     3841 ctacaactac atctagtatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     3901 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     3961 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     4021 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     4081 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     4141 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     4201 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     4261 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     4321 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     4381 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4441 tgctgcccga caaccactac ctgagtactc agtccaagct gagcaaagac cccaacgaga
     4501 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     4561 acgagctgta caagagtaac actagtcaat tggcaacttt gtataataaa gttgaacgag
     4621 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
     4681 cataatactg taaaacacaa catatccagt cactatggcg gccgcgggct cactcattag
     4741 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     4801 taacaatttc acacaggaaa cagctatgac catgattacc ccgtccctgc acgcctgtcg
     4861 ttcaaccctg gaagatccgc gtgtcccgag ttctaacagt ctggccgtcg ttctgcagcg
     4921 tcgcgattgg gaaaacccgg gcgtgaccca actgaatcgt ctggcggccc atccgccgtt
     4981 tgcgtcgtgg cgtaactcag aagaagcccg taccgaccgt ccgtctcaac aactgcgctc
     5041 ctaacactat aaaaagctga tgcagacggg gcttttttct gcaggtcgac catagtgact
     5101 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     5161 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgtcta
     5221 ggttaccagt atacccttat gatgttcctg attatgctgg ttacccttac gatgttcctg
     5281 attacgcagg atctgtatac ccttacgatg ttccagatta tgcctaagcg gccatgctag
     5341 agtccgcaaa aatcaccagt ctctctctac aaatctatct ctctctattt ttctccagaa
     5401 taatgtgtga gtagttccca gataagggaa ttagggttct tatagggttt cgctcatgtg
     5461 ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa
     5521 atttctaatt cctaaaacca aaatccagtg aagggtagat catgagcgga gaattaaggg
     5581 agtcacgtta tgacccccgc cgatgacgcg ggacaagccg ttttacgttt ggaactgaca
     5641 gaaccgcaac gttgaaggag ccactgagcg cgggtttctg gagtttaatg agctaagcac
     5701 atacgtcaga aaccattatt gcgcgttcaa aagtcgccta aggtcactat catctagcaa
     5761 atatttcttg tcaaaaatgc tccactgacg ttccataaat tcccctcggt atccaattag
     5821 agtctcatat tcactctcaa ctcgatcgag gggatctacc atgagtccag aaaggagacc
     5881 agcagatatt aggagagcaa ccgaagcaga tatgccagca gtttgcacca ttgtgaacca
     5941 ttacatcgag acttctacag ttaattttag gactgaacct caagagccac aggaatggac
     6001 agatgatctc gtgagattaa gggaaagata cccttggctt gttgctgagg tggatggaga
     6061 agttgcaggt attgcttacg caggaccttg gaaggctaga aacgcttatg attggactgc
     6121 tgagtctacc gtttacgtgt cacctagaca tcaaagaacc ggacttggtt caaccttgta
     6181 tactcacctt ttgaagtctc ttgaagctca gggattcaaa tctgttgtgg ctgttatcgg
     6241 tttgcctaat gatccaagtg tgagaatgca tgaagctctc ggatacgcac caaggggtat
     6301 gttaagagct gctggattta aacatggtaa ctggcacgat gttggtttct ggcagttaga
     6361 tttcagttta ccagttccac caagaccagt gcttccagtg accgagattt gacccgggct
     6421 agagtcaagc agatcgttca aacatttggc aataaagttt cttaatattg aatcctgttg
     6481 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     6541 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     6601 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     6661 cggtgtcatc tatgttacta gatcgaccgg catgaagctg agttaacgat gtaagtcctc
     6721 aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
     6781 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
     6841 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
     6901 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
     6961 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
     7021 aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc
     7081 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
     7141 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     7201 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     7261 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     7321 aaagttaaac atcatggggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     7381 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     7441 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     7501 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     7561 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     7621 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     7681 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     7741 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     7801 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     7861 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     7921 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     7981 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     8041 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     8101 gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc cgacgccgct
     8161 tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa
     8221 ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt
     8281 gggagatata tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8341 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8401 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8461 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     8521 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8581 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8641 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8701 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8761 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8821 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8881 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8941 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9001 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9061 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9121 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     9181 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     9241 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     9301 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     9361 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     9421 aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg
     9481 ccctggtaga ttgcctggcc ctaggccagc catttttgag cggccagcgg ccgcgatagg
     9541 ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag
     9601 ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt
     9661 aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac
     9721 ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt
     9781 cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg
     9841 acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc
     9901 ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc
     9961 cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt
    10021 gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa
    10081 ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat
    10141 gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc
    10201 tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt
    10261 ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag
    10321 gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat
    10381 gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag
    10441 tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta
    10501 tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac
    10561 actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg
    10621 gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat
    10681 gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc
    10741 gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc
    10801 tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat
    10861 gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc
    10921 cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg
    10981 ggggttccag tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg
    11041 cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc
    11101 atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc
    11161 tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc
    11221 gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc
    11281 tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc
    11341 ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt
    11401 tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct
    11461 gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata
    11521 cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg
    11581 cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc
    11641 tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt
    11701 ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc
    11761 gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg
    11821 tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc
    11881 caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt
    11941 tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta
    12001 agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca
    12061 tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac
    12121 ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag
    12181 acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca
    12241 cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct
    12301 ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag
    12361 ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg
    12421 cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag
    12481 tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat
    12541 ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc
    12601 gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa
    12661 cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct
    12721 acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct
    12781 gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag
    12841 catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt
    12901 ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct
    12961 gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg
    13021 gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga
    13081 aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga
    13141 agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa
    13201 agtactttaa agtactttaa agtactttaa agtactttga tcccgagggg aaccctgtgg
    13261 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
    13321 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
    13381 tgatagttt
//
