LOCUS       sequence-204248-       13336 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET/FLIM analysis in plants (stable or transient transformation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13336)
  AUTHORS   Hecker A, Wallmeroth N, Peter S, Blatt MR, Harter K, Grefen C
  TITLE     Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic 
            Protein Interaction Studies.
  JOURNAL   Plant Physiol. 2015 Jul;168(3):776-87. doi: 10.1104/pp.15.00533. 
            Epub 2015 May 13.
  PUBMED    25971551
REFERENCE   2  (bases 1 to 13336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.15.00533"; journalName: "Plant Physiol"; date: 
            "2015-07"; volume: "168"; issue: "3"; pages: "776-87"
COMMENT     Sequence Label: pFRETtv-2in1-NC
FEATURES             Location/Qualifiers
     source          1..13336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        143..487
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    581..705
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(730..735,736..753,754..760)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             814..1473
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(880..899)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1815..2120
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1999..2018
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2161..2285)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(2305..2307,2308..2310,2311..3024)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        3373..3717
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(3805..3807,3808..3810,3811..4521)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    4541..4664
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4699..4704,4705..4722,4723..4729)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4737..4753
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4742..4764
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4761..4777
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4761..4777
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(5038..5162)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5179..5205
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5209..5235
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5245..5271
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5588..5766
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      6379..6631
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6736..6760
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7281..8072
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8318..8906
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8807..8826
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9060..9077
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9092..9232
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9158..9177)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9318..9340
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9576..9770
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9836..10909)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11338..11967)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13298..13322
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aaactgaagg cgggaaacga caatctgatc caagctcaag ctaagctagg actagccctc
       61 tgcaggtcaa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag
      121 tctcagaaga ccagagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc
      181 tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaagatg
      241 gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctaccg
      301 acagtggtcc caaagatgga cccccaccca cgaggaacat cgtggaaaaa gaagacgttc
      361 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
      421 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
      481 agaggacctc gagcgcgcgt atttttaaac aattaccaac aacaacaaca aacaacaaca
      541 acattacatt ttacattcta caactacatc tagtcctaga acaagtttgt acaaaaaagc
      601 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
      661 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc
      721 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
      781 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
      841 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
      901 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
      961 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     1021 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     1081 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     1141 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     1201 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     1261 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     1321 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     1381 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     1441 cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt actaaaagcc
     1501 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg
     1561 tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg
     1621 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag
     1681 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
     1741 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa
     1801 caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
     1861 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc
     1921 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
     1981 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
     2041 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
     2101 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
     2161 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
     2221 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt tctatacaaa
     2281 gttggtcaat tgttaggcac tagtatggtg agcaagggcg aggagctgtt caccggggtg
     2341 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     2401 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc
     2461 aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct gcagtgcttc
     2521 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     2581 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     2641 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     2701 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     2761 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
     2821 gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     2881 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag caaggatccc
     2941 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     3001 ggcatggacg agctgtacaa gtaattataa gcggccatgc tagagtccgc aaaaatcacc
     3061 agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc
     3121 ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac
     3181 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa
     3241 ccaaaatcca gtgacctagg aggctcgcac gtgcggagct ccgtaccctc tgcaggtcaa
     3301 catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga
     3361 ccagagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca
     3421 ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaagatg gcttctacaa
     3481 atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctaccg acagtggtcc
     3541 caaagatgga cccccaccca cgaggaacat cgtggaaaaa gaagacgttc caaccacgtc
     3601 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
     3661 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agaggacctc
     3721 gagcgcgcgt atttttaaac aattaccaac aacaacaaca aacaacaaca acattacatt
     3781 ttacattcta caactacatc tagtatggtg agcaagggcg aggagctgtt caccggggtg
     3841 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3901 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3961 aagctgcccg tgccctggcc caccctcgtg accaccctgt cctggggcgt gcagtgcttc
     4021 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     4081 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     4141 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     4201 gaggacggca acatcctggg gcacaagctg gagtacaact actttagcga caacgtctat
     4261 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
     4321 gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     4381 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc
     4441 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     4501 ggcatggacg agctgtacaa gagtaacact agtcaattgg caactttgta taataaagtt
     4561 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
     4621 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gcgggctcac
     4681 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     4741 gagcggataa caatttcaca caggaaacag ctatgaccat gattaccccg tccctgcacg
     4801 cctgtcgttc aaccctggaa gatccgcgtg tcccgagttc taacagtctg gccgtcgttc
     4861 tgcagcgtcg cgattgggaa aacccgggcg tgacccaact gaatcgtctg gcggcccatc
     4921 cgccgtttgc gtcgtggcgt aactcagaag aagcccgtac cgaccgtccg tctcaacaac
     4981 tgcgctccta acactataaa aagctgatgc agacggggct tttttctgca ggtcgaccat
     5041 agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa
     5101 tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg
     5161 gtgtctaggt taccagtata cccttatgat gttcctgatt atgctggtta cccttacgat
     5221 gttcctgatt acgcaggatc tgtataccct tacgatgttc cagattatgc ctaagcggcc
     5281 atgctagagt ccgcaaaaat caccagtctc tctctacaaa tctatctctc tctatttttc
     5341 tccagaataa tgtgtgagta gttcccagat aagggaatta gggttcttat agggtttcgc
     5401 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
     5461 caataaaatt tctaattcct aaaaccaaaa tccagtgaag ggtagatcat gagcggagaa
     5521 ttaagggagt cacgttatga cccccgccga tgacgcggga caagccgttt tacgtttgga
     5581 actgacagaa ccgcaacgtt gaaggagcca ctgagcgcgg gtttctggag tttaatgagc
     5641 taagcacata cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg tcactatcat
     5701 ctagcaaata tttcttgtca aaaatgctcc actgacgttc cataaattcc cctcggtatc
     5761 caattagagt ctcatattca ctctcaactc gatcgagggg atctaccatg agtccagaaa
     5821 ggagaccagc agatattagg agagcaaccg aagcagatat gccagcagtt tgcaccattg
     5881 tgaaccatta catcgagact tctacagtta attttaggac tgaacctcaa gagccacagg
     5941 aatggacaga tgatctcgtg agattaaggg aaagataccc ttggcttgtt gctgaggtgg
     6001 atggagaagt tgcaggtatt gcttacgcag gaccttggaa ggctagaaac gcttatgatt
     6061 ggactgctga gtctaccgtt tacgtgtcac ctagacatca aagaaccgga cttggttcaa
     6121 ccttgtatac tcaccttttg aagtctcttg aagctcaggg attcaaatct gttgtggctg
     6181 ttatcggttt gcctaatgat ccaagtgtga gaatgcatga agctctcgga tacgcaccaa
     6241 ggggtatgtt aagagctgct ggatttaaac atggtaactg gcacgatgtt ggtttctggc
     6301 agttagattt cagtttacca gttccaccaa gaccagtgct tccagtgacc gagatttgac
     6361 ccgggctaga gtcaagcaga tcgttcaaac atttggcaat aaagtttctt aatattgaat
     6421 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta
     6481 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
     6541 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
     6601 tcgcgcgcgg tgtcatctat gttactagat cgaccggcat gaagctgagt taacgatgta
     6661 agtcctcaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg
     6721 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
     6781 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
     6841 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
     6901 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
     6961 tgattgtaac gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga
     7021 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     7081 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     7141 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     7201 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     7261 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     7321 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     7381 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     7441 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     7501 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     7561 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     7621 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     7681 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     7741 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     7801 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     7861 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     7921 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     7981 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     8041 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     8101 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     8161 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac
     8221 acaaattggg agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact
     8281 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8341 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8401 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8461 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8521 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8581 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8641 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8701 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8761 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8821 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8881 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8941 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     9001 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     9061 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     9121 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     9181 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     9241 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     9301 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     9361 acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt
     9421 gtccgcgccc tggtagattg cctggcccta ggccagccat ttttgagcgg ccagcggccg
     9481 cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt
     9541 tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa
     9601 gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat
     9661 cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt
     9721 tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac
     9781 cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg
     9841 aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag
     9901 cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc
     9961 gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct
    10021 tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac
    10081 ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct
    10141 tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc
    10201 gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg
    10261 tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta
    10321 accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga
    10381 acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt
    10441 cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat
    10501 cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct
    10561 cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt
    10621 ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg
    10681 gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc
    10741 gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg
    10801 cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac
    10861 ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc
    10921 gggcttgggg gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg
    10981 ccaaccgccc gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg
    11041 attttccatg ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca
    11101 tttactctgg tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc
    11161 gtaccgcgta catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct
    11221 tcatggctgg cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg
    11281 gacggccggc acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca
    11341 aatgagtttt gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc
    11401 gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg
    11461 cgtgatacgg gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc
    11521 gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc
    11581 cgtgacctca atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta
    11641 agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa
    11701 gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac
    11761 gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac
    11821 ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc
    11881 ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt
    11941 ctggttaagt acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat
    12001 cgcatcatat acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt
    12061 tttagacggc ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg
    12121 gcatcagaca aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc
    12181 tcgaacacgt acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt
    12241 tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg
    12301 ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct
    12361 cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc
    12421 caagcagtgc agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt
    12481 gcgcgatctg tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg
    12541 cggcctcgcg cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc
    12601 cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg
    12661 ccaggctacg caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc
    12721 gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt
    12781 ggtcaagcat cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa
    12841 acagcttggt gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt
    12901 cggtgctgac gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat
    12961 gtctccggtt ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac
    13021 gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt
    13081 tttcagaaga cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt
    13141 ggatcaaagt actttaaagt actttaaagt actttaaagt actttgatcc cgaggggaac
    13201 cctgtggttg gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa
    13261 atatccgatt attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt
    13321 caaacactga tagttt
//
