LOCUS       sequence-204250-       13329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET/FLIM analysis in plants (stable or transient transformation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13329)
  AUTHORS   Hecker A, Wallmeroth N, Peter S, Blatt MR, Harter K, Grefen C
  TITLE     Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic 
            Protein Interaction Studies.
  JOURNAL   Plant Physiol. 2015 Jul;168(3):776-87. doi: 10.1104/pp.15.00533. 
            Epub 2015 May 13.
  PUBMED    25971551
REFERENCE   2  (bases 1 to 13329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.15.00533"; journalName: "Plant Physiol"; date: 
            "2015-07"; volume: "168"; issue: "3"; pages: "776-87"
COMMENT     Sequence Label: pFRETvr-2in1-NC
FEATURES             Location/Qualifiers
     source          1..13329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        142..486
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    580..704
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(729..734,735..752,753..759)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             813..1472
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(879..898)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1814..2119
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1998..2017
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2160..2284)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3366..3710
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(3798..3800,3801..3803,3804..4514)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3843..3864)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4104..4123)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4451..4472
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    4534..4657
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4692..4697,4698..4715,4716..4722)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4730..4746
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4735..4757
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4754..4770
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4754..4770
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(5031..5155)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5172..5198
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5202..5228
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5238..5264
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5581..5759
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      6372..6624
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6729..6753
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7274..8065
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8311..8899
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8800..8819
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9053..9070
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9085..9225
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9151..9170)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9311..9333
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9569..9763
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9829..10902)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11331..11960)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13291..13315
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aaactgaagg cgggaaacga caatctgatc caagctcaag ctaagctagg actagcctct
       61 gcaggtcaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
      121 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
      181 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg
      241 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga
      301 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc
      361 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
      421 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
      481 gaggacctcg agcgcgcgta tttttaaaca attaccaaca acaacaacaa acaacaacaa
      541 cattacattt tacattctac aactacatct agtcctagaa caagtttgta caaaaaagct
      601 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
      661 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca
      721 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
      781 cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
      841 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
      901 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
      961 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
     1021 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
     1081 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
     1141 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
     1201 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
     1261 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
     1321 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
     1381 aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
     1441 agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca
     1501 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt
     1561 atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga
     1621 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc
     1681 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag
     1741 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac
     1801 aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
     1861 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
     1921 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
     1981 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
     2041 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
     2101 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
     2161 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
     2221 aatttaatat attgatattt atatcatttt acgtttctcg ttcaactttt ctatacaaag
     2281 ttggtcaatt gttaggcact agtatggtga gtaaaggtga agagttgatt aaagagaaca
     2341 tgcatatgaa gttatacatg gagggaactg tcaataatca ccactttaag tgtacatcag
     2401 agggtgaggg aaagccatac gagggaaccc aaactatgag aatcaaagta gtggagggag
     2461 gtcctcttcc atttgctttt gatatactag caacaagttt catgtatggt tccaggacct
     2521 tcattaacca tactcaggga atccctgact tctttaaaca gtcttttcct gaaggtttta
     2581 catgggagag ggttaccact tacgaggacg gtggagtctt gacagcaacc caggacactt
     2641 cattacaaga tggatgccta atatacaatg tgaaaattag gggtgtgaat ttccctagta
     2701 acggaccagt tatgcagaag aaaacactag gttgggaagc taatactgaa atgttgtacc
     2761 ctgccgacgg aggtttagaa ggtagatccg acatggccct taagctagtc ggaggtggac
     2821 acttgatctg taactttaaa accacatata ggtctaagaa gccagcaaag aatctaaaaa
     2881 tgcctggtgt ttactatgtg gaccatagac tagaaaggat aaaagaagca gacaaagaaa
     2941 cttacgtgga gcaacatgag gtcgccgtcg ctaggtattg cgacttacct tccaagctag
     3001 gtcacaaatt gaactgatta taagcggcca tgctagagtc cgcaaaaatc accagtctct
     3061 ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag ttcccagata
     3121 agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt
     3181 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat
     3241 ccagtgacct aggaggctcg cacgtgcgga gctccgtacc ctctgcaggt caacatggtg
     3301 gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccagagg
     3361 gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca
     3421 gctatctgtc acttcatcga aaggacagta gaaaaggaag atggcttcta caaatgccat
     3481 cattgcgata aaggaaaggc tatcgttcaa gatgcctcta ccgacagtgg tcccaaagat
     3541 ggacccccac ccacgaggaa catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
     3601 caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct
     3661 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac ctcgagcgcg
     3721 cgtattttta aacaattacc aacaacaaca acaaacaaca acaacattac attttacatt
     3781 ctacaactac atctagtatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     3841 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     3901 agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc
     3961 ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct
     4021 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     4081 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     4141 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     4201 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg
     4261 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg
     4321 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4381 tgctgcccga caaccactac ctgagctacc agtccaagct gagcaaggat cccaacgaga
     4441 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     4501 acgagctgta caagagtaac actagtcaat tggcaacttt gtataataaa gttgaacgag
     4561 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
     4621 cataatactg taaaacacaa catatccagt cactatggcg gccgcgggct cactcattag
     4681 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     4741 taacaatttc acacaggaaa cagctatgac catgattacc ccgtccctgc acgcctgtcg
     4801 ttcaaccctg gaagatccgc gtgtcccgag ttctaacagt ctggccgtcg ttctgcagcg
     4861 tcgcgattgg gaaaacccgg gcgtgaccca actgaatcgt ctggcggccc atccgccgtt
     4921 tgcgtcgtgg cgtaactcag aagaagcccg taccgaccgt ccgtctcaac aactgcgctc
     4981 ctaacactat aaaaagctga tgcagacggg gcttttttct gcaggtcgac catagtgact
     5041 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     5101 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgtcta
     5161 ggttaccagt atacccttat gatgttcctg attatgctgg ttacccttac gatgttcctg
     5221 attacgcagg atctgtatac ccttacgatg ttccagatta tgcctaagcg gccatgctag
     5281 agtccgcaaa aatcaccagt ctctctctac aaatctatct ctctctattt ttctccagaa
     5341 taatgtgtga gtagttccca gataagggaa ttagggttct tatagggttt cgctcatgtg
     5401 ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa
     5461 atttctaatt cctaaaacca aaatccagtg aagggtagat catgagcgga gaattaaggg
     5521 agtcacgtta tgacccccgc cgatgacgcg ggacaagccg ttttacgttt ggaactgaca
     5581 gaaccgcaac gttgaaggag ccactgagcg cgggtttctg gagtttaatg agctaagcac
     5641 atacgtcaga aaccattatt gcgcgttcaa aagtcgccta aggtcactat catctagcaa
     5701 atatttcttg tcaaaaatgc tccactgacg ttccataaat tcccctcggt atccaattag
     5761 agtctcatat tcactctcaa ctcgatcgag gggatctacc atgagtccag aaaggagacc
     5821 agcagatatt aggagagcaa ccgaagcaga tatgccagca gtttgcacca ttgtgaacca
     5881 ttacatcgag acttctacag ttaattttag gactgaacct caagagccac aggaatggac
     5941 agatgatctc gtgagattaa gggaaagata cccttggctt gttgctgagg tggatggaga
     6001 agttgcaggt attgcttacg caggaccttg gaaggctaga aacgcttatg attggactgc
     6061 tgagtctacc gtttacgtgt cacctagaca tcaaagaacc ggacttggtt caaccttgta
     6121 tactcacctt ttgaagtctc ttgaagctca gggattcaaa tctgttgtgg ctgttatcgg
     6181 tttgcctaat gatccaagtg tgagaatgca tgaagctctc ggatacgcac caaggggtat
     6241 gttaagagct gctggattta aacatggtaa ctggcacgat gttggtttct ggcagttaga
     6301 tttcagttta ccagttccac caagaccagt gcttccagtg accgagattt gacccgggct
     6361 agagtcaagc agatcgttca aacatttggc aataaagttt cttaatattg aatcctgttg
     6421 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     6481 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     6541 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     6601 cggtgtcatc tatgttacta gatcgaccgg catgaagctg agttaacgat gtaagtcctc
     6661 aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
     6721 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
     6781 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
     6841 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
     6901 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
     6961 aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc
     7021 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
     7081 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     7141 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     7201 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     7261 aaagttaaac atcatggggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     7321 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     7381 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     7441 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     7501 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     7561 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     7621 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     7681 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     7741 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     7801 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     7861 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     7921 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     7981 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     8041 gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc cgacgccgct
     8101 tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa
     8161 ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt
     8221 gggagatata tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8281 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8341 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8401 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     8461 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8521 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8581 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8641 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8701 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8761 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8821 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8881 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8941 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9001 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9061 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     9121 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     9181 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     9241 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     9301 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     9361 aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg
     9421 ccctggtaga ttgcctggcc ctaggccagc catttttgag cggccagcgg ccgcgatagg
     9481 ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag
     9541 ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt
     9601 aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac
     9661 ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt
     9721 cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg
     9781 acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc
     9841 ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc
     9901 cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt
     9961 gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa
    10021 ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat
    10081 gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc
    10141 tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt
    10201 ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag
    10261 gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat
    10321 gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag
    10381 tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta
    10441 tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac
    10501 actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg
    10561 gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat
    10621 gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc
    10681 gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc
    10741 tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat
    10801 gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc
    10861 cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg
    10921 ggggttccag tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg
    10981 cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc
    11041 atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc
    11101 tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc
    11161 gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc
    11221 tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc
    11281 ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt
    11341 tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct
    11401 gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata
    11461 cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg
    11521 cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc
    11581 tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt
    11641 ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc
    11701 gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg
    11761 tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc
    11821 caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt
    11881 tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta
    11941 agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca
    12001 tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac
    12061 ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag
    12121 acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca
    12181 cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct
    12241 ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag
    12301 ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg
    12361 cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag
    12421 tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat
    12481 ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc
    12541 gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa
    12601 cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct
    12661 acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct
    12721 gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag
    12781 catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt
    12841 ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct
    12901 gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg
    12961 gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga
    13021 aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga
    13081 agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa
    13141 agtactttaa agtactttaa agtactttaa agtactttga tcccgagggg aaccctgtgg
    13201 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
    13261 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
    13321 tgatagttt
//
