LOCUS       sequence-204254-        6458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a soma-targeted GtACR1 in Cre-expressing neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Mahn M, Gibor L, Patil P, Cohen-Kashi Malina K, Oring S, Printz Y, 
            Levy R, Lampl I, Yizhar O
  TITLE     High-efficiency optogenetic silencing with soma-targeted 
            anion-conducting channelrhodopsins.
  JOURNAL   Nat Commun. 2018 Oct 8;9(1):4125. doi: 10.1038/s41467-018-06511-8.
  PUBMED    30297821
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-10-8"; volume: "9(1):4125. doi"; pages: " 
            10.1038/s41467-018-06511-8"
COMMENT     Sequence Label: pAAV_hSyn1-SIO-stGtACR1-FusionRed
FEATURES             Location/Qualifiers
     source          1..6458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..622
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    complement(636..669)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(877..1572)
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     protein_bind    2539..2572
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2585..3173
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2638..2658)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3056..3067)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3205..3681
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3404..3423)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3721..3861
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3721..3850
                     /label=AAV2 ITR
     rep_origin      3936..4391
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4023..4042)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4233..4254
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4408..4427)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4527..4549
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4587..4605)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4673..4777
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4778..4846,4847..5638)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4996..5015)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5809..6397
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6298..6317
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca
      181 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
      241 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
      301 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
      361 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
      421 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
      481 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
      541 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
      601 cgtgtcgtgc ctgagagcgc agtcgagaag gtacctaccg ttcgtatagc atacattata
      661 cgaagttatg aattcttact taaaccgcgt agcctctgga aaatcggtgg cgcatgagat
      721 aaaagagtca atagaactca tggacctcat gtcgatcacg ccctctgttc ttgcaggaag
      781 aggtgccaca ggggagggca ttgaggacat ttccagctcc tcagggggtt tactctgggg
      841 ggccatctct ttggtgttca gaataggctg ggactgttta cctccatcac cagcgccacc
      901 agtagagtat ctggccacag ccacctcgtg ctgctcgacg tatgtctcat cgtcggcctc
      961 cttgattctt tccagtctgt ggtccacgtt gtagacgccg ggcatcttga ggttcgtagc
     1021 gggtttcttg gatctgtatg ttgtctcaag gttgcagatc aggtgtcctc ctcccacgag
     1081 cttcagagcc atgtcacatg cgccttccag tcctccgtca gcagggtaca tcgtctcggt
     1141 ggaggcctcc cagccgagtg ttttcttctg catcacaggg ccgttggctg ggaagttcac
     1201 ccctctaacc ttgacgttgt agatgaggca gccgtcctgg aggctggtgt cctgggtagc
     1261 ggtcagcacg cccccgtctt cgtatgtggt gactctctcc catgtgaagc cctcagggaa
     1321 ggactgctta aagaagtcgg ggatgcccgg agggtgcttg atgaaggttc tgctgccgta
     1381 catgaagctg gtagccagga tgtcgaaggc gaaggggaga gggccgccct cgacgacctt
     1441 gattctcatg gtctgggtgc cctcgtaggg cttgccttcg ccctcggatg tgcacttgaa
     1501 gtggtggttg ttcacggtgc cctccatgta cagcttcatg ggcatgttct ccttaatcag
     1561 ctcgctcacc atcaccacgt tgatgtcgat ctggtccagg gggatgtact cgccctcgct
     1621 ggtgatcctg ctcttggcgg cggtttcccc agcccgagca tcctcatatc gttcagtatt
     1681 gcctttctcc agatcctgtt catattcggg catcagtgtc ccatcgacat tgcgtccttt
     1741 cacgtaatct cggtcccact tgccgttcag cagtccccag gtagtagccc acagcaggat
     1801 tccataggta tttttgcaca gtgcgtcgcc cagcaggtac agcacagagg aagtgttctc
     1861 agacataatg caagcccctg tgcttgaaaa actccacagg ataggatagg ccatccagct
     1921 cacgtagaac acgattctca tcagtttcag cctttcgacc accttcagtg acatgggact
     1981 tccgatggtc tgaaaatcat gaatagtgat ggcaaaaatg gcgaatgcga ccaccatctc
     2041 aaagataaag aaaatactag agatgaaaca gtacagccac acgtgcaggg ggtccaggac
     2101 cacggaggca gtgattccga acactgtgca gatgctgctg gcggcaatca tcatgggatt
     2161 cagtggaatg gatttgtact tcaccagggc catgttgctc accaggccca gcatgattgg
     2221 acatgtgcac agccaacttg ccattctagc ccaaggcaga tacttcccat ttgccactcg
     2281 aatgtatccg ttgcctgtga aagccagtga ataggtgatc atttctgtgg ttggcaggta
     2341 gatggcctcc caaggcactt tgggtgccct agtgaccata aagaacacct ggcaggcgct
     2401 caccacggcc atgaccagct gcacgtactt gatgccgtcc agggtaatca ggctcttttc
     2461 gacgcaaaac tggttctctc tgctccactc tccatagatg gctgggtcac aagtaatgct
     2521 gctcatggtg gcggatccta ccgttcgtat aatgtatgct atacgaagtt ataagcttat
     2581 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     2641 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     2701 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     2761 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     2821 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     2881 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     2941 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     3001 gctcgcctat gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     3061 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     3121 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     3181 ccgagcgctg ctcgagagat ctacgggtgg catccctgtg acccctcccc agtgcctctc
     3241 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc
     3301 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg
     3361 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc
     3421 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt
     3481 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa
     3541 tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc
     3601 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc
     3661 actgctccct tccctgtcct tctgattttg taggtaacca cgtgcggacc gagcggccgc
     3721 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     3781 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
     3841 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg
     3901 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     3961 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc
     4021 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4081 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4141 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4201 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4261 actcaactct atctcgggct attcttttga tttataaggg attttgccga tttcggtcta
     4321 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4381 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     4441 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     4501 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     4561 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     4621 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     4681 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4741 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     4801 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     4861 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4921 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4981 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5041 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5101 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5161 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     5221 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     5281 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     5341 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     5401 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     5461 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     5521 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     5581 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     5641 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5701 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5761 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5821 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5881 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5941 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6001 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6061 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6121 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6181 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6241 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6301 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6361 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6421 acggttcctg gccttttgct ggccttttgc tcacatgt
//
