LOCUS       sequence-204261-        9853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To study localization and function of Tensin1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Clark K, Howe JD, Pullar CE, Green JA, Artym VV, Yamada KM, 
            Critchley DR
  TITLE     Tensin 2 modulates cell contractility in 3D collagen gels through 
            the RhoGAP DLC1.
  JOURNAL   J Cell Biochem. 2010 Mar 1;109(4):808-17. doi: 10.1002/jcb.22460.
  PUBMED    20069572
REFERENCE   2  (bases 1 to 9853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/jcb"; 
            journalName: "J Cell Biochem"; date: "2010-03-1- 1"; volume: "109"; 
            issue: "4"; pages: "808-17"
COMMENT     Sequence Label: pEGFP Tensin1
FEATURES             Location/Qualifiers
     source          1..9853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      49..637
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     538..557
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        801..1104
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1105..1308
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1259..1279
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1347..1356
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1353..1355,1356..1358,1359..2069)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1353..1355,1356..1358,1359..2069)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1398..1419)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1659..1678)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2006..2027
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7392..7513
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7429..7448)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7483..7502
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7520..7975)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7657..7678)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7869..7888
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8002..8106
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8108..8465
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8108..8299
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8131..8151)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8316..8451
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8500..9294
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8500..9294
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8554..8573)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9164..9183
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9482..9501)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9526..9573
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
       61 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
      121 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
      181 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
      241 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
      301 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      361 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      421 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      481 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      541 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      601 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      661 acggttcctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
      721 accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat
      781 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      841 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      901 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      961 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     1021 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1081 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     1141 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     1201 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     1261 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
     1321 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca
     1381 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     1441 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     1501 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     1561 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     1621 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     1681 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     1741 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     1801 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     1861 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1921 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     1981 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     2041 tcactctcgg catggacgag ctgtacaagt ccggactcag atctcgagct caagcttaca
     2101 tgagtgtgag ccggaccatg gacagctgtg agctggacct ggtgtacgtc acagagagga
     2161 tcatcgctgt ctccttcccc agcacagcca atgaggagaa cttccggagc aacctccgtg
     2221 aggtggcgca gatgctcaag tccaaacatg gaggcaacta cctgctgttc aacctctctg
     2281 agcggagacc tgacatcacg aagctccatg ccaaggtact ggaatttggc tggcccgacc
     2341 tccacacccc agccctggag aagatctgca gcatctgtaa ggccatggac acatggctca
     2401 atgcagaccc tcacaatgtc gttgttctac acaacaaggg aaaccgaggc aggataggag
     2461 ttgtcatcgc ggcttacatg cactacagca acatttctgc cagtgcggac caggctctgg
     2521 accagtttgc aatgaagcgg ttctatgagg ataagattgt gcccattggc cagccatccc
     2581 aaagaaggta cgtgcattac ttcagtggcc tgctctccgg ctccatcaaa atgaacaaca
     2641 agcccttgtt tctgcaccac gtgatcatgc acggcatccc caactttgag tctaaaggag
     2701 gatgtcggcc atttctccgc atctaccagg ccatgcaacc tgtgtacaca tctggcatct
     2761 acaacatccc aggagacagc cagactagcg tctgcatcac catcgagcca ggactgctct
     2821 tgaagggaga catcttgctg aagtgctacc acaagaagtt ccgaagccca gcccgagacg
     2881 tcatcttccg tgtgcagttc cacacctgtg ccatccatga cctgggggtt gtctttggga
     2941 aggaggacct tgatgatgct ttcaaagatg atcgatttcc agagtatggc aaagtggagt
     3001 ttgtattttc ttatgggcca gagaaaattc aaggcatgga gcacctggag aacgggccga
     3061 gcgtgtctgt ggactataac acctctgacc ccctcatccg ctgggactcc tacgacaact
     3121 tcagtgggca tcgagatgac ggcatggagg aggtggtggg acacacgcag gggccactag
     3181 atgggagcct gtatgctaag gtgaagaaga aagactccct gcacggcagc accggggctg
     3241 ttaatgccac acgtcctaca ctgtcggcca cccccaacca cgtggaacac acgctttctg
     3301 tgagcagcga ctcgggcaac tccacagcct ccaccaagac cgacaagacc gacgagcctg
     3361 tccccggggc ctccagtgcc actgctgcct tgagtcccca ggagaagcgg gagctggacc
     3421 gcctgctgag tggctttggc ttagagcgag agaagcaagg cgccatgtac cacacccagc
     3481 acctcaggtc ccgcccagca gggggctcgg ctgtgccctc ctctggacgc cacgttgtcc
     3541 cagcccaggt tcatgtcaat ggtggggcgt tagcatctga gcgggagaca gacatcctgg
     3601 acgatgaatt gccaaaccag gatggtcaca gtgcgggcag catgggcaca ctctcttctc
     3661 tggacggggt caccaacacc agtgaggggg gctacccaga ggccctgtcc ccactgacca
     3721 acggtctgga caagtcctac cccatggagc ctatggtcaa tggaggaggc tacccctacg
     3781 agtctgccag ccgggcgggg cctgcccatg ctggccacac ggcccccatg cggccctcct
     3841 actctgcaca ggagggttta gctggctacc agagggaggg gccccaccca gcctggccac
     3901 agccagtgac cacctcccac tatgcccatg accccagcgg tatgttccgc tctcaatcct
     3961 tttcggaagc tgaaccccag ctgcccccag ctccggtccg agggggaagc agccgggagg
     4021 ctgtgcaaag gggactgaat tcatggcagc agcagcagca gcagcagcag cagcctcgcc
     4081 cacctccacg ccagcaggaa agagcccact tggagagtct tgtagccagc aggcccagcc
     4141 ctcagccatt ggcagagacc cccatcccca gtctccctga gttcccgcga gcagcctccc
     4201 agcaggagat tgaacagtcc atcgaaacac tcaatatgct gatgctggac ctggagccag
     4261 cctccgctgc tgccccacta cacaagtccc agagtgtccc cggggcctgg ccaggggctt
     4321 ctccactctc ctcccagccc ctctctggat cctcccgtca gtcccatcca ctgacccagt
     4381 ccagatctgg ctatatcccc agtgggcatt cgttgggaac ccctgagcca gccccacggg
     4441 cctctctgga gtctgtccct cctggcaggt cttactcacc ttatgactat cagccatgtt
     4501 tggctgggcc taaccaggat ttccattcaa agagcccagc ctcttcctcc ttgcctgcct
     4561 tccttccgac cacccacagc cctccagggc ctcagcaacc cccagcctct ctccctggcc
     4621 tcactgctca gcctctgctc tcaccaaagg aagcgacttc agacccctcc cggactccag
     4681 aggaggagcc attgaattta gaagggctgg tggcccacag ggtagcaggg gtacaggctc
     4741 gggagaagca gcctgcagag cccccagccc ctctgcggag gcgggcggcc agtgatggac
     4801 agtatgagaa ccagtctcca gaagccacat cccctcgtag ccctggggtt cgctcccctg
     4861 tccagtgtgt ctccccggag ctggctctta ccatcgctct caatcctgga gggcggccca
     4921 aagagcccca tttgcacagc tacaaggagg ccttcgagga gatggaggga acctccccga
     4981 gcagcccacc acccagtggg gtgcggtccc ccccgggtct ggccaagaca cccctgtctg
     5041 ctctgggcct gaaacctcac aacccagcgg acatcctgtt gcaccccaca ggagagcccc
     5101 ggagctatgt ggagtctgtg gcacggacag cggtggctgg accccgagct caggactctg
     5161 agcccaagag ctttagtgct ccagccaccc aggcctatgg ccatgagata cccctgagga
     5221 acgggaccct gggtggctcc tttgtctccc ccagccccct ctccaccagc agccccatcc
     5281 tcagtgctga cagcacttca gtggggagtt tcccgtcggg agagagcagt gaccagggtc
     5341 cccggacgcc cacccagcct ctgttggagt ctggcttccg ctcaggcagc ctgggacagc
     5401 ccagcccatc tgcccagaga aactaccaga gctcttctcc tctcccgact gtgggcagta
     5461 gctacagcag ccccgactac tcacttcagc atttcagctc ctctccggaa agccaggccc
     5521 gagctcagtt cagtgtggct ggcgtccaca cggtgcctgg gagccctcag gcgcgccaca
     5581 gaacagtggg caccaacact ccccctagtc ctggcttcgg ccggcgggcc atcaatccca
     5641 gcatggctgc ccccagcagt cccagtttga gccatcacca gatgatgggt ccaccaggca
     5701 ctggcttcca tggtagcact gtctccagcc cccagagcag tgcagcgacc accccgggga
     5761 gccccagcct gtgtcggcac ccagcagggg tctaccaggt ttctggcctc cacaacaaag
     5821 tggccaccac cccggggagt cccagcctgg gccggcaccc tggggctcac caaggcaacc
     5881 tggcctccgg tcttcatagc aatgcaatag ccagccctgg aagccccagc ctgggccgtc
     5941 acctcggagg gtctggatct gtggttcccg gcagcccctg cttggaccgg catgtggcct
     6001 atggcggcta ttctaccccg gaggatcgga gacccacact gtcccggcag agcagtgcct
     6061 ctggctacca ggctccttcc acgccctcct tccctgtctc ccctgcctac taccctggcc
     6121 tgagcagccc tgccacctcc ccgtcaccag actccgcagc cttccggcaa gggagcccaa
     6181 caccagcctt gccagagaag cgaaggatgt cagtgggaga ccgggcaggc agcctcccca
     6241 actatgccac catcaatggg aaggtgtctt cgcctgtcgc cagcggcatg tccagtccca
     6301 gcgggggcag caccgtctcc ttctcccaca ctctgcccga cttctccaag tactccatgc
     6361 cagacaacag cccggagacg cgggctaaag tgaagtttgt ccaggacact tctaagtatt
     6421 ggtacaagcc tgagatctcc agggagcagg ccatcgcgct cctcaaggac caggagccgg
     6481 gggccttcat catccgcgac agtcactcct tccgaggcgc gtacgggctg gccatgaagg
     6541 tgtcttcgcc acctccaacc atcatgcagc agaataaaaa aggagacatg acccatgagc
     6601 tggtcaggca ttttctgata gagactggcc ccagaggagt caagctcaag ggctgcccca
     6661 atgagccaaa cttcggatcg ctgtctgccc tggtctacca gcactccatc atcccattgg
     6721 ccctgccttg caagctggtc attccaaacc gagaccccac agatgaatcg aaagatagct
     6781 ccggccctgc caactcaact gcagacctgc tgaaacaagg ggcagcctgc aatgtgctct
     6841 tcatcaactc tgtggacatg gagtcactca ctgggccaca ggccatctct aaagccacat
     6901 ctgagacgtt ggctgcagac cccacaccag ctgccaccat cgttcacttc aaagtctctg
     6961 cccagggaat cactctgact gacaaccaga gaaagctctt tttcagacgc cactaccctc
     7021 tcaacactgt caccttctgt gacctggatc cacaggaaag aaagtggatg aaaacagagg
     7081 gtggtgcccc tgctaagctc ttcggcttcg tggcccggaa gcagggcagc accacggaca
     7141 acgcctgcca cctctttgct gagcttgacc ccaaccagcc ggcctctgcc atcgtcaact
     7201 tcgtctccaa ggtcatgctg aatgccggcc aaaagagatg agtcgacggt accgcgggcc
     7261 cgggatccac cggatctaga taactgatca taatcagcca taccacattt gtagaggttt
     7321 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     7381 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7441 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7501 tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     7561 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     7621 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     7681 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     7741 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     7801 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     7861 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     7921 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt
     7981 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     8041 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     8101 aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     8161 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8221 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     8281 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     8341 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     8401 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     8461 gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     8521 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     8581 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     8641 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
     8701 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     8761 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     8821 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     8881 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     8941 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     9001 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc
     9061 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     9121 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     9181 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     9241 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     9301 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     9361 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     9421 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa
     9481 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     9541 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     9601 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
     9661 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     9721 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc
     9781 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9841 cttaacgtga gtt
//
