LOCUS       sequence-204274-       13403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET/FLIM analysis in plants (stable or transient transformation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13403)
  AUTHORS   Hecker A, Wallmeroth N, Peter S, Blatt MR, Harter K, Grefen C
  TITLE     Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic 
            Protein Interaction Studies.
  JOURNAL   Plant Physiol. 2015 Jul;168(3):776-87. doi: 10.1104/pp.15.00533. 
            Epub 2015 May 13.
  PUBMED    25971551
REFERENCE   2  (bases 1 to 13403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.15.00533"; journalName: "Plant Physiol"; date: 
            "2015-07"; volume: "168"; issue: "3"; pages: "776-87"
COMMENT     Sequence Label: pFRETtv-2in1-NN
FEATURES             Location/Qualifiers
     source          1..13403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        143..487
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(575..577,578..580,581..1291)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    1310..1434
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1459..1464,1465..1482,1483..1489)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1543..2202
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1609..1628)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2544..2849
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2728..2747
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2890..3014)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3040..3069
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3070..3099
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        3440..3784
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(3872..3874,3875..3877,3878..4588)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    4608..4731
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4766..4771,4772..4789,4790..4796)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4804..4820
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4809..4831
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4828..4844
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4828..4844
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(5105..5229)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5246..5272
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5276..5302
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5312..5338
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5655..5833
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      6446..6698
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6803..6827
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7348..8139
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8385..8973
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8874..8893
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9127..9144
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9159..9299
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9225..9244)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9385..9407
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9643..9837
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9903..10976)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11405..12034)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13365..13389
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aaactgaagg cgggaaacga caatctgatc caagctcaag ctaagctagg actagccctc
       61 tgcaggtcaa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag
      121 tctcagaaga ccagagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc
      181 tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaagatg
      241 gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctaccg
      301 acagtggtcc caaagatgga cccccaccca cgaggaacat cgtggaaaaa gaagacgttc
      361 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
      421 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
      481 agaggacctc gagcgcgcgt atttttaaac aattaccaac aacaacaaca aacaacaaca
      541 acattacatt ttacattcta caactacatc tagtatggtg agcaagggcg aggagctgtt
      601 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      661 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg
      721 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct
      781 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      841 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      901 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      961 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     1021 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
     1081 ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
     1141 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag
     1201 caaggatccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1261 gatcactctc ggcatggacg agctgtacaa gagtaacact agtcctagaa caagtttgta
     1321 caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
     1381 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc
     1441 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
     1501 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
     1561 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
     1621 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
     1681 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
     1741 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
     1801 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
     1861 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
     1921 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1981 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     2041 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     2101 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     2161 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     2221 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     2281 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     2341 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     2401 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     2461 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     2521 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     2581 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     2641 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     2701 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     2761 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     2821 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     2881 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     2941 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcaactttt
     3001 ctatacaaag ttggtcaatt gttaggcggt ggatctctcg agcagaagtt gatctcagag
     3061 gaggacctcg agcagaagtt gatctcagag gaggacttat aagcggccat gctagagtcc
     3121 gcaaaaatca ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg
     3181 tgtgagtagt tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag
     3241 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     3301 taattcctaa aaccaaaatc cagtgaaggg ctcgcacgtg cggagctccg taccctctgc
     3361 aggtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
     3421 cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
     3481 gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct
     3541 tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctaccgaca
     3601 gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa
     3661 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
     3721 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
     3781 ggacctcgag cgcgcgtatt tttaaacaat taccaacaac aacaacaaac aacaacaaca
     3841 ttacatttta cattctacaa ctacatctag tatggtgagc aagggcgagg agctgttcac
     3901 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     3961 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     4021 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgtcct ggggcgtgca
     4081 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     4141 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     4201 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     4261 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactact ttagcgacaa
     4321 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     4381 caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     4441 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
     4501 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     4561 cactctcggc atggacgagc tgtacaagag taacactagt caattggcaa ctttgtataa
     4621 taaagttgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca
     4681 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggcggccgcg
     4741 ggctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     4801 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat taccccgtcc
     4861 ctgcacgcct gtcgttcaac cctggaagat ccgcgtgtcc cgagttctaa cagtctggcc
     4921 gtcgttctgc agcgtcgcga ttgggaaaac ccgggcgtga cccaactgaa tcgtctggcg
     4981 gcccatccgc cgtttgcgtc gtggcgtaac tcagaagaag cccgtaccga ccgtccgtct
     5041 caacaactgc gctcctaaca ctataaaaag ctgatgcaga cggggctttt ttctgcaggt
     5101 cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     5161 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta
     5221 caaagtggtg tctaggttac cagtataccc ttatgatgtt cctgattatg ctggttaccc
     5281 ttacgatgtt cctgattacg caggatctgt atacccttac gatgttccag attatgccta
     5341 agcggccatg ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct
     5401 atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg
     5461 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
     5521 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgaagggt agatcatgag
     5581 cggagaatta agggagtcac gttatgaccc ccgccgatga cgcgggacaa gccgttttac
     5641 gtttggaact gacagaaccg caacgttgaa ggagccactg agcgcgggtt tctggagttt
     5701 aatgagctaa gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca
     5761 ctatcatcta gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct
     5821 cggtatccaa ttagagtctc atattcactc tcaactcgat cgaggggatc taccatgagt
     5881 ccagaaagga gaccagcaga tattaggaga gcaaccgaag cagatatgcc agcagtttgc
     5941 accattgtga accattacat cgagacttct acagttaatt ttaggactga acctcaagag
     6001 ccacaggaat ggacagatga tctcgtgaga ttaagggaaa gatacccttg gcttgttgct
     6061 gaggtggatg gagaagttgc aggtattgct tacgcaggac cttggaaggc tagaaacgct
     6121 tatgattgga ctgctgagtc taccgtttac gtgtcaccta gacatcaaag aaccggactt
     6181 ggttcaacct tgtatactca ccttttgaag tctcttgaag ctcagggatt caaatctgtt
     6241 gtggctgtta tcggtttgcc taatgatcca agtgtgagaa tgcatgaagc tctcggatac
     6301 gcaccaaggg gtatgttaag agctgctgga tttaaacatg gtaactggca cgatgttggt
     6361 ttctggcagt tagatttcag tttaccagtt ccaccaagac cagtgcttcc agtgaccgag
     6421 atttgacccg ggctagagtc aagcagatcg ttcaaacatt tggcaataaa gtttcttaat
     6481 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     6541 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     6601 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     6661 taaattatcg cgcgcggtgt catctatgtt actagatcga ccggcatgaa gctgagttaa
     6721 cgatgtaagt cctcaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat
     6781 taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac
     6841 agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc
     6901 gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc
     6961 tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt
     7021 agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc
     7081 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     7141 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     7201 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     7261 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     7321 agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga agtatcgact
     7381 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     7441 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     7501 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     7561 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     7621 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     7681 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     7741 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     7801 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     7861 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     7921 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     7981 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     8041 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     8101 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag aaattcgttc
     8161 aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat
     8221 tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa
     8281 gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg tgagttttcg
     8341 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8401 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8461 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8521 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8581 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8641 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8701 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8761 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8821 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8881 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8941 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     9001 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     9061 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     9121 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     9181 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     9241 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     9301 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     9361 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     9421 caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc
     9481 gcggcttgtc cgcgccctgg tagattgcct ggccctaggc cagccatttt tgagcggcca
     9541 gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta
     9601 ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg
     9661 gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct
     9721 tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg
     9781 tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga
     9841 aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta
     9901 cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc
     9961 gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc
    10021 agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag
    10081 atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag
    10141 agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc
    10201 gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac
    10261 tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg
    10321 gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg
    10381 gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc
    10441 cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc
    10501 ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg
    10561 tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct
    10621 ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg
    10681 gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa
    10741 aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc
    10801 ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt
    10861 gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg
    10921 tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg
    10981 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta
    11041 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
    11101 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
    11161 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
    11221 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
    11281 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
    11341 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
    11401 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
    11461 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
    11521 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
    11581 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
    11641 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
    11701 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
    11761 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
    11821 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
    11881 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
    11941 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
    12001 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
    12061 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
    12121 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
    12181 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
    12241 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
    12301 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
    12361 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc
    12421 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga
    12481 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg
    12541 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg
    12601 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg
    12661 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac
    12721 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt
    12781 aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg
    12841 cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc
    12901 tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc
    12961 tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg
    13021 gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca
    13081 agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac
    13141 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg
    13201 aggggttgga tcaaagtact ttaaagtact ttaaagtact ttaaagtact ttgatcccga
    13261 ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc ttttcacgcc
    13321 cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac ccgccaatat
    13381 atcctgtcaa acactgatag ttt
//
