LOCUS       sequence-204284-       14951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Sp dCas9 and GA-inducible VPR for OR gate/diametric 
            regulation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 14951)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 14951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE
            PGK-...
FEATURES             Location/Qualifiers
     source          1..14951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     115..137
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     199..218
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      246..255
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1314..1463
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             1470..1490
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1920..2291
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             2310..2879
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     misc_feature    join(2897..3470,3471..3473,3474..3482)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3064..3081)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3291..3310
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3504..4103
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3504..3523)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4000..4020
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4141..4729
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4194..4214)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4612..4623)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4750..5249
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4785..4808)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5182..5201
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5291..5308
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5582..6280
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     regulatory      6296..6305
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6302..6328,6329..6331,6332..8818,8819..8821,
                     8822..10405)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6302..10405
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10409..10435
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             10415..10441
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             10454..10474
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10481..10501
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     repeat_region   11639..11701
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(11752..11772)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11752..11770)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11791..11807)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11791..11807)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11804..11826)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11815..11831
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11839..11845,11846..11863,11864..11869))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11884..11905
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12022..12039)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12193..12781)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12273..12292)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12952..13743,13744..13812))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13575..13594
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13813..13917)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(13943..14398)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14080..14101)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14292..14311
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14525..14547
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14539..14556
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14540..14556
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14566..14585
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14566..14584
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   14614..14648
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
       61 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
      121 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
      181 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
      241 aattcgccac catggctgcg agcgatgaag ttaatcttat tgagagcaga acagtggttc
      301 ctctcaatac atgggtttta atatccaact tcaaagtagc ctacaatatc cttcgtcgcc
      361 ctgatggaac ctttaaccga cacttagctg agtatctaga ccgtaaagtc actgcaaacg
      421 ccaatccggt tgatggggtt ttctcgttcg atgtcttgat tgatcgcagg atcaatcttc
      481 taagcagagt ctatagacca gcttatgcag atcaagagca acctcctagt attttagatc
      541 tcgagaagcc tgttgatggc gacattgtcc ctgttatatt gttcttccat ggaggtagct
      601 ttgctcattc ttctgcaaac agtgccatct acgatactct ttgtcgcagg cttgttggtt
      661 tgtgcaagtg tgttgttgtc tctgtgaatt atcggcgtgc accagagaat ccataccctt
      721 gtgcttatga tgatggttgg attgctctta attgggttaa ctcgagatct tggcttaaat
      781 ccaagaaaga ctcaaaggtc catattttct tggctggtga tagctctgga ggtaacatcg
      841 cgcataatgt ggctttaaga gcgggtgaat cgggaatcga tgttttgggg aacattctgc
      901 tgaatcctat gtttggtggg aatgagagaa cggagtctga gaaaagtttg gatgggaaat
      961 actttgtgac ggttagagac cgcgattggt actggaaagc gtttttaccc gagggagaag
     1021 atagagagca tccagcgtgt aatccgttta gcccgagagg gaaaagctta gaaggagtga
     1081 gtttccccaa gagtcttgtg gttgtcgcgg gtttggattt gattagagat tggcagttgg
     1141 catacgcgga agggctcaag aaagcgggtc aagaggttaa gcttatgcat ttagagaaag
     1201 caactgttgg gttttacctc ttgcctaata acaatcattt ccataatgtt atggatgaga
     1261 tttcggcgtt tgtaaacgcg gaatgtgaat tctcaagcgc tgcaggtact agtgatgctt
     1321 tagacgattt tgacttagat atgcttggtt cagacgcgtt agacgacttc gacctagaca
     1381 tgttaggctc agatgcattg gacgacttcg atttagatat gttgggctcc gatgccctag
     1441 atgactttga tctagatatg ctaggtagtc ccaaaaagaa gaggaaagtg ggatcccagt
     1501 atctgcccga cacagatgat agacaccgaa tcgaagagaa acgcaagcga acgtatgaaa
     1561 ccttcaaatc gatcatgaag aaatcgccct tctcgggtcc gaccgatccc aggcccccac
     1621 cgagaaggat tgcggtcccg tcccgctcgt cggccagcgt gccgaagcct gcgccgcagc
     1681 cctacccctt cacgtcgagc ctgagcacaa tcaattatga cgagttcccg acgatggtgt
     1741 tcccctcggg acaaatctca caagcctcgg cgctcgcacc agcgcctccc caagtccttc
     1801 cgcaagcgcc tgccccagcg cctgcaccgg caatggtgtc cgccctcgca caggcccctg
     1861 cgcccgtccc cgtgctcgcg cctggaccgc cccaggcggt cgctccaccg gctccgaagc
     1921 cgacgcaggc cggagaggga acactctccg aagcacttct tcaactccag tttgatgacg
     1981 aggatcttgg agcactcctt ggaaactcga cagaccctgc ggtgtttacc gacctcgcgt
     2041 cagtagataa ctccgaattt cagcagcttt tgaaccaggg tatcccggtc gcgccacata
     2101 caacggagcc catgttgatg gaataccccg aagcaatcac gagacttgtg acgggagcgc
     2161 agcggcctcc cgatcccgca cccgcacctt tgggggcacc tggcctccct aacggacttt
     2221 tgagcggcga cgaggatttc tcctccatcg ccgatatgga tttctcagcc ttgctgtcac
     2281 agatttccag cggctctggc agcggcagcc gggattccag ggaagggatg tttttgccga
     2341 agcctgaggc cggctccgct attagtgacg tgtttgaggg ccgcgaggtg tgccagccaa
     2401 aacgaatccg gccatttcat cctccaggaa gtccatgggc caaccgccca ctccccgcca
     2461 gcctcgcacc aacaccaacc ggtccagtac atgagccagt cgggtcactg accccggcac
     2521 cagtccctca gccactggat ccagcgcccg cagtgactcc cgaggccagt cacctgttgg
     2581 aggatcccga tgaagagacg agccaggctg tcaaagccct tcgggagatg gccgatactg
     2641 tgattcccca gaaggaagag gctgcaatct gtggccaaat ggacctttcc catccgcccc
     2701 caaggggcca tctggatgag ctgacaacca cacttgagtc catgaccgag gatctgaacc
     2761 tggactcacc cctgaccccg gaattgaacg agattctgga taccttcctg aacgacgagt
     2821 gcctcttgca tgccatgcat atcagcacag gactgtccat cttcgacaca tctctgtttt
     2881 gaatttaaat aattcgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     2941 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     3001 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     3061 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     3121 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     3181 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     3241 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     3301 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     3361 atctggggcc tcggtgcaca tgctttacgt gtgtttagtc gaggttaaaa aacgtctagg
     3421 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     3481 ccggatccgg agctaccatg accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     3541 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     3601 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     3661 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     3721 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     3781 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     3841 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     3901 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     3961 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     4021 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     4081 tgacccgcaa gcccggtgcc tgagtttaaa ccatgcaagc ttgatatcaa gcttatcgat
     4141 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4201 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4261 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4321 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4381 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4441 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4501 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     4561 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4621 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4681 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcctgcaggt
     4741 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     4801 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     4861 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     4921 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     4981 aagtagcacg tctcactagg ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     5041 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     5101 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     5161 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     5221 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttgtcg acgccaccat
     5281 gaagagagat catcatcatc atcatcatca agataagaag actatgatga tgaatgaaga
     5341 agacgacggt aacggcatgg atgagcttct agctgttctt ggttacaagg ttaggtcatc
     5401 cgaaatggct gatgttgctc agaaactcga gcagcttgaa gttatgatgt ctaatgttca
     5461 agaagacgat ctttctcaac tcgctactga gactgttcac tataatccgg cggagcttta
     5521 cacgtggctt gattctatgc tcaccgacct taatcatatg tcaagcgctg caggtgctac
     5581 catgagcgag ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga
     5641 caaccatcac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac
     5701 catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac
     5761 tagcttcctc tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt
     5821 caagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg
     5881 cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa
     5941 gatcagaggg gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg
     6001 ggaggccttc accgagacgc tgtaccccgc tgacggcggc ctggaaggca gaaacgacat
     6061 ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac atcaagacca catatagatc
     6121 caagaaaccc gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga
     6181 aagaatcaag gaggccaaca acgagaccta cgtcgagcag cacgaggtgg cagtggccag
     6241 atactgcgac ctccctagca aactggggca caagcttaat tcaagcgctg caggtgccac
     6301 catggacaag aagtacagca tcggcctggc catcggcacc aactctgtgg gctgggccgt
     6361 gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg
     6421 gcacagcatc aagaagaacc tgatcggcgc cctgctgttc gacagcggag aaacagccga
     6481 ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg
     6541 ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag
     6601 actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg
     6661 caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa
     6721 gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca
     6781 catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga
     6841 cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc
     6901 catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag
     6961 acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggcaa
     7021 cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga
     7081 ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc
     7141 ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat
     7201 cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc
     7261 tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg
     7321 gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc
     7381 cggctacatc gatggcggag ccagccagga agagttctac aagttcatca agcccatcct
     7441 ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg
     7501 gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca
     7561 cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat
     7621 cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag
     7681 cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga
     7741 agtggtggac aagggcgcca gcgcccagag cttcatcgag cggatgacca acttcgataa
     7801 gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt
     7861 gtacaacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct
     7921 gagcggcgag cagaaaaaag ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac
     7981 cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat
     8041 ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat
     8101 tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt
     8161 gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc
     8221 ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg
     8281 caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct
     8341 ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga
     8401 cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct
     8461 gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac
     8521 agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt
     8581 gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag
     8641 aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc
     8701 cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg
     8761 ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg atgtggacgc
     8821 tatcgtgcct cagagctttc tgaaggacga ctccatcgat aacaaagtgc tgactcggag
     8881 cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa
     8941 gaactactgg cgccagctgc tgaatgccaa gctgattacc cagaggaagt tcgacaatct
     9001 gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca
     9061 gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa
     9121 cactaagtac gacgagaacg acaaactgat ccgggaagtg aaagtgatca ccctgaagtc
     9181 caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa
     9241 ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa
     9301 gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa
     9361 gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag
     9421 caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg
     9481 gcctctgatc gagacaaacg gcgaaacagg cgagatcgtg tgggataagg gccgggactt
     9541 tgccaccgtg cggaaagtgc tgtctatgcc ccaagtgaat atcgtgaaaa agaccgaggt
     9601 gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg acaagctgat
     9661 cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc ccaccgtggc
     9721 ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt
     9781 gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga
     9841 ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa
     9901 gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccggcgaact
     9961 gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag
    10021 ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga
    10081 acagcacaaa cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt
    10141 gatcctggcc gacgctaatc tggacaaggt gctgagcgcc tacaacaagc acagagacaa
    10201 gcctatcaga gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc
    10261 ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca ccagcaccaa
    10321 agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat
    10381 cgacctgtct cagctgggag gcgacgccta tccctatgac gtgcccgatt atgccagcct
    10441 gggcagcggc tcccccaaga aaaaacgcaa ggtggaagat cctaagaaaa agcggaaagt
    10501 ggacggcatt ggtagtggga gcaacggagg tggaggctct ggacctagga cgcgtgtgcc
    10561 tttgtatggt tttacttcga tttgtggaag aagacctgag atggaagctg ctgtttcgac
    10621 tataccaaga ttccttcaat cttcctctgg ttcgatgtta gatggtcggt ttgatcctca
    10681 atccgccgct catttcttcg gtgtttacga cggccatggc ggttctcagg tagcgaacta
    10741 ttgtagagag aggatgcatt tggctttggc ggaggagata gctaaggaga aaccgatgct
    10801 ctgcgatggt gatacgtggc tggagaagtg gaagaaagct cttttcaact cgttcctgag
    10861 agttgactcg gagattgagt cagttgcgcc ggagacggtt gggtcaacgt cggtggttgc
    10921 cgttgttttc ccgtctcaca tcttcgtcgc taactgcggt gactctagag ccgttctttg
    10981 ccgcggcaaa actgcacttc cattatccgt tgaccataaa ccggatagag aagatgaagc
    11041 tgcgaggatt gaagccgcag gagggaaagt gattcagtgg aatggagctc gtgttttcgg
    11101 tgttctcgcc atgtcgagat ccattggcga tagatacttg aaaccatcca tcattcctga
    11161 tccggaagtg acggctgtga agagagtaaa agaagatgat tgtctgattt tggcgagtga
    11221 cggggtttgg gatgtaatga cggatgaaga agcgtgtgag atggcaagga agcggattct
    11281 cttgtggcac aagaaaaacg cggtggctgg ggatgcatcg ttgctcgcgg atgagcggag
    11341 aaaggaaggg aaagatcctg cggcgatgtc cgcggctgag tatttgtcaa agctggcgat
    11401 acagagagga agcaaagaca acataagtgt ggtggtggtt gatttgaagt gactcgagct
    11461 agttaaaagt tttgttactt tatagaagaa attttgagtt tttgtttttt tttaataaat
    11521 aaataaacat aaataaattg tttgttgaat ttattattag tatgtaagtg taaatataat
    11581 aaaacttaat atctattcaa attaataaat aaacctcgat atacagaccg ataaaacaca
    11641 tgcgtcaatt ttacgcatga ttatctttaa cgtacgtcac aatatgatta tctttctagg
    11701 gttaatctag tatacgcgta tgcggccgct taattaatcc agcttttgtt ccctttagtg
    11761 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    11821 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    11881 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    11941 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    12001 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    12061 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    12121 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    12181 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    12241 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    12301 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    12361 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    12421 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    12481 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    12541 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    12601 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
    12661 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    12721 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    12781 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    12841 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    12901 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    12961 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    13021 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    13081 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    13141 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    13201 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    13261 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    13321 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    13381 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    13441 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    13501 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    13561 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    13621 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    13681 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    13741 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    13801 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    13861 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    13921 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
    13981 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
    14041 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
    14101 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
    14161 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
    14221 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
    14281 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
    14341 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
    14401 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
    14461 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
    14521 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
    14581 tagggcgaat tggggcgcgc cattctagat taaccctaga aagatagtct gcgtaaaatt
    14641 gacgcatgca ttcttgaaat attgctctct ctttctaaat agcgcgaatc cgtcgctgtg
    14701 catttaggac atctcagtcg ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta
    14761 agtgtcactg attttgaact ataacgaccg cgtgagtcaa aatgacgcat gattatcttt
    14821 tacgtgactt ttaagattta actcatacga taattatatt gttatttcat gttctactta
    14881 cgtgataact tattatatat atattttctt gttatagata tcaactagaa tgctagcatg
    14941 ggcccagatc t
//
