LOCUS       sequence-204301-        9403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of Flag-tagged SMAD4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9403)
  AUTHORS   David CJ, Huang YH, Chen M, Su J, Zou Y, Bardeesy N, 
            Iacobuzio-Donahue CA, Massague J
  TITLE     TGF-beta Tumor Suppression through a Lethal EMT.
  JOURNAL   Cell. 2016 Feb 25;164(5):1015-30. doi: 10.1016/j.cell.2016.01.009. 
            Epub 2016 Feb 18.
  PUBMED    26898331
REFERENCE   2  (bases 1 to 9403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.01.009"; journalName: "Cell"; date: 
            "2016-02-25- 25"; volume: "164"; issue: "5"; pages: "1015-30"
COMMENT     Sequence Label: pLVX-Tight-Puro FLAG-SMAD4 TetON
FEATURES             Location/Qualifiers
     source          1..9403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2205..2519
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2213..2231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2249..2267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2284..2302
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2320..2338
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2356..2374
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2391..2409
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2427..2445
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2492..2516
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2533..2542
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2542..2565
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        4235..4734
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4270..4293)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4667..4686
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4755..5354
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4755..4774)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5251..5271
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5371..5959
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5424..5444)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5842..5853)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6166..6799
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6927..6943)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6927..6943)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6940..6962)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6951..6967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6975..6981,6982..6999,7000..7005))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7020..7041
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7158..7175)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7329..7917)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7409..7428)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8088..8879,8880..8948))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8711..8730
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8949..9053)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9101..9235
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9138..9157)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9192..9211
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(9403,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgaggccc tttcgtcttc actcgagttt actccctatc
     2221 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg atgtcgagtt
     2281 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     2341 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta tccctatcag
     2401 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgaggta
     2461 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     2521 ggagaaggat ccgccaccat ggattacaag gacgacgatg acaagatgga caatatgtct
     2581 attacgaata caccaacaag taatgatgcc tgtctgagca ttgtgcatag tttgatgtgc
     2641 catagacaag gtggagagag tgaaacattt gcaaaaagag caattgaaag tttggtaaag
     2701 aagctgaagg agaaaaaaga tgaattggat tctttaataa cagctataac tacaaatgga
     2761 gctcatccta gtaaatgtgt taccatacag agaacattgg atgggaggct tcaggtggct
     2821 ggtcggaaag gatttcctca tgtgatctat gcccgtctct ggaggtggcc tgatcttcac
     2881 aaaaatgaac taaaacatgt taaatattgt cagtatgcgt ttgacttaaa atgtgatagt
     2941 gtctgtgtga atccatatca ctacgaacga gttgtatcac ctggaattga tctctcagga
     3001 ttaacactgc agagtaatgc tccatcaagt atgatggtga aggatgaata tgtgcatgac
     3061 tttgagggac agccatcgtt gtccactgaa ggacattcaa ttcaaaccat ccagcatcca
     3121 ccaagtaatc gtgcatcgac agagacatac agcaccccag ctctgttagc cccatctgag
     3181 tctaatgcta ccagcactgc caactttccc aacattcctg tggcttccac aagtcagcct
     3241 gccagtatac tggggggcag ccatagtgaa ggactgttgc agatagcatc agggcctcag
     3301 ccaggacagc agcagaatgg atttactggt cagccagcta cttaccatca taacagcact
     3361 accacctgga ctggaagtag gactgcacca tacacaccta atttgcctca ccaccaaaac
     3421 ggccatcttc agcaccaccc gcctatgccg ccccatcccg gacattactg gcctgttcac
     3481 aatgagcttg cattccagcc tcccatttcc aatcatcctg ctcctgagta ttggtgttcc
     3541 attgcttact ttgaaatgga tgttcaggta ggagagacat ttaaggttcc ttcaagctgc
     3601 cctattgtta ctgttgatgg atacgtggac ccttctggag gagatcgctt ttgtttgggt
     3661 caactctcca atgtccacag gacagaagcc attgagagag caaggttgca cataggcaaa
     3721 ggtgtgcagt tggaatgtaa aggtgaaggt gatgtttggg tcaggtgcct tagtgaccac
     3781 gcggtctttg tacagagtta ctacttagac agagaagctg ggcgtgcacc tggagatgct
     3841 gttcataaga tctacccaag tgcatatata aaggtctttg atttgcgtca gtgtcatcga
     3901 cagatgcagc agcaggcggc tactgcacaa gctgcagcag ctgcccaggc agcagccgtg
     3961 gcaggaaaca tccctggccc aggatcagta ggtggaatag ctccagctat cagtctgtca
     4021 gctgctgctg gaattggtgt tgatgacctt cgtcgcttat gcatactcag gatgagtttt
     4081 gtgaaaggct ggggaccgga ttacccaaga cagagcatca aagaaacacc ttgctggatt
     4141 gaaattcact tacaccgggc cctccagctc ctagacgaag tacttcatac catgccgatt
     4201 gcagacccac aacctttaga ctgagaattc taccgggtag gggaggcgct tttcccaagg
     4261 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     4321 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     4381 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     4441 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     4501 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     4561 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     4621 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     4681 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     4741 agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     4801 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     4861 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     4921 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     4981 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     5041 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     5101 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     5161 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     5221 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     5281 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     5341 agcccggtgc ctgacgggcg cgtctggaac aatcaacctc tggattacaa aatttgtgaa
     5401 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     5461 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     5521 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     5581 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     5641 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     5701 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     5761 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     5821 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     5881 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     5941 ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag
     6001 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     6061 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
     6121 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc
     6181 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     6241 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     6301 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
     6361 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
     6421 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     6481 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     6541 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     6601 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     6661 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     6721 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6781 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     6841 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt accgtcgacc
     6901 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6961 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7021 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7081 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7141 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7201 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7261 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7321 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7381 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7441 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7501 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7561 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7621 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7681 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7741 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7801 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7861 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7921 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7981 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8041 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8101 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8161 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8221 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8281 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8341 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8401 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8461 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8521 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8581 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8641 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8701 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8761 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8821 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8881 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8941 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9001 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9061 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta
     9121 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9181 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
     9241 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
     9301 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
     9361 aagaaattgt atttgttaaa tatgtactac aaacttagta gtt
//
