LOCUS       sequence-204318-        5021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  hMEF2C phosphomimetic obtained from Quikchange Lightning 
            Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Brown FC, Still E, Koche RP, Yim CY, Takao S, Cifani P, Reed C, 
            Gunasekera S, Ficarro SB, Romanienko P, Mark W, McCarthy C, de 
            Stanchina E, Gonen M, Seshan V, Bhola P, O'Donnell C, Spitzer B, 
            Stutzke C, Lavallee VP, Hebert J, Krivtsov AV, Melnick A, Paietta 
            EM, Tallman MS, Letai A, Sauvageau G, Pouliot G, Levine R, Marto JA,
            Armstrong SA, Kentsis A
  TITLE     MEF2C Phosphorylation Is Required for Chemotherapy Resistance in 
            Acute Myeloid Leukemia.
  JOURNAL   Cancer Discov. 2018 Apr;8(4):478-497. doi: 
            10.1158/2159-8290.CD-17-1271. Epub 2018 Feb 5.
  PUBMED    29431698
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-17-1271"; journalName: "Cancer Discov"; date: 
            "2018-04"; volume: "8"; issue: "4"; pages: "478-497"
COMMENT     Sequence Label: MSCV-IRES_Tomato MEF2C S222D
FEATURES             Location/Qualifiers
     source          1..5021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    262..603
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             670..1086
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1016..1038
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1054..1070
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1104..1113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(2558..3131,3132..3134,3135..3143)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2725..2742)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2952..2971
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3144..4574
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggcgcgccag
       61 tcctccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc ttgctgtttg
      121 catccgaatc gtggactcgc tgatccttgg gagggtctcc tcagattgat tgactgccca
      181 cctcgggggt ctttcatttg gaggttccac cgagatttgg agacccctgc ctagggacca
      241 ccgacccccc cgccgggagg taagctggcc agcggtcgtt tcgtgtctgt ctctgtcttt
      301 gtgcgtgttt gtgccggcat ctaatgtttg cgcctgcgtc tgtactagtt agctaactag
      361 ctctgtatct ggcggacccg tggtggaact gacgagttct gaacacccgg ccgcaaccct
      421 gggagacgtc ccagggactt tgggggccgt ttttgtggcc cgacctgagg aagggagtcg
      481 atgtggaatc cgaccccgtc aggatatgtg gttctggtag gagacgagaa cctaaaacag
      541 ttcccgcctc cgtctgaatt tttgctttcg gtttggaacc gaagccgcgc gtcttgtctg
      601 ctgcagcgct gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct
      661 gaaaattagg gccagactgt taccactccc ttaagtttga ccttaggtca ctggaaagat
      721 gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg ggttaccttc
      781 tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac ctttaaccga
      841 gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg acacccagac
      901 caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc ctgggtcaag
      961 ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc tctccccctt
     1021 gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac tccttctcta
     1081 ggcgccggaa ttagatctct cgagccacca tggggagaaa aaagattcag attacgagga
     1141 ttatggatga acgtaacaga caggtgacat ttacaaagag gaaatttggg ttgatgaaga
     1201 aggcttatga gctgagcgtg ctgtgtgact gtgagattgc gctgatcatc ttcaacagca
     1261 ccaacaagct gttccagtat gccagcaccg acatggacaa agtgcttctc aagtacacgg
     1321 agtacaacga gccgcatgag agccggacaa actcagacat cgtggagacg ttgagaaaga
     1381 agggccttaa tggctgtgac agcccagacc ccgatgcgga cgattccgta ggtcacagcc
     1441 ctgagtctga ggacaagtac aggaaaatta acgaagatat tgatctaatg atcagcaggc
     1501 aaagattgtg tgctgttcca cctcccaact tcgagatgcc agtctccatc ccagtgtcca
     1561 gccacaacag tttggtgtac agcaaccctg tcagctcact gggaaacccc aacctattgc
     1621 cactggctca cccttctctg cagaggaata gtatgtctcc tggtgtaaca catcgacctc
     1681 caagtgcagg taacacaggt ggtctgatgg gtggagacct cacgtctggt gcaggcacca
     1741 gtgcagggaa cgggtatggc aatccccgaa acgacccagg tctgctggtc tcacctggta
     1801 acttgaacaa gaatatgcaa gcaaaatctc ctcccccaat gaatttagga atgaataacc
     1861 gtaaaccaga tctccgagtt cttattccac caggcagcaa gaatacgatg ccatcagtgt
     1921 ctgaggatgt cgacctgctt ttgaatcaaa ggataaataa ctcccagtcg gctcagtcat
     1981 tggctacccc agtggtttcc gtagcaactc ctactttacc aggacaagga atgggaggat
     2041 atccatcagc catttcaaca acatatggta cggagtactc tctgagtagt gcagacctgt
     2101 catctctgtc tgggtttaac accgccagcg ctcttcacct tggttcagta actggctggc
     2161 aacagcaaca cctacataac atgccaccat ctgccctcag tcagttggga gcttgcacta
     2221 gcactcattt atctcagagt tcaaatctct ccctgccttc tactcaaagc ctcaacatca
     2281 agtcagaacc tgtttctcct cctagagacc gtaccaccac cccttcgaga tacccacaac
     2341 acacgcgcca cgaggcgggg agatctcctg ttgacagctt gagcagctgt agcagttcgt
     2401 acgacgggag cgaccgagag gatcaccgga acgaattcca ctcccccatt ggactcacca
     2461 gaccttcgcc ggacgaaagg gaaagtccct cagtcaagcg catgcgactt tctgaaggat
     2521 gggcaacata aggatccccc gggctgcagg aattccgccc ctctccctcc ccccccccta
     2581 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     2641 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     2701 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     2761 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     2821 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     2881 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     2941 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     3001 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     3061 cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     3121 acgatgataa tatggccaca accatggtga gcaagggcga ggaggtcatc aaagagttca
     3181 tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
     3241 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg
     3301 gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccaaggcgt
     3361 acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc gagggcttca
     3421 agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc caggactcct
     3481 ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg
     3541 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc
     3601 cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag gacggcggcc
     3661 actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa ctgcccggct
     3721 actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg
     3781 aacagtacga gcgctccgag ggccgccacc acctgttcct ggggcatggc accggcagca
     3841 ccggcagcgg cagctccggc accgcctcct ccgaggacaa caacatggcc gtcatcaaag
     3901 agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg
     3961 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     4021 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca
     4081 aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg
     4141 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg
     4201 actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc
     4261 cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc
     4321 tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg
     4381 gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc
     4441 ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca
     4501 tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgtac ggcatggacg
     4561 agctgtacaa gtaagaattc gatatcaagc ttatcgataa aataaaagat tttatttagt
     4621 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaatta
     4681 acgccatttt gcaaggcatg gaaaatacat aactgagaat agagaagttc agatcaaggt
     4741 taggaacaga gagacagcag aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     4801 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tcccgccctc agcagtttct
     4861 agagaaccat cagatgtttc cagggtgccc caaggacctg aaaatgaccc tgtgccttat
     4921 ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc
     4981 aataaaagag cccacaaccc ctcactcggc gcgccagtcc t
//
