LOCUS       sequence-204366-        7848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of myc-His tagged AXL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Krishnamoorthy GP, Guida T, Alfano L, Avilla E, Santoro M, 
            Carlomagno F, Melillo RM
  TITLE     Molecular mechanism of 17-allylamino-17-demethoxygeldanamycin 
            (17-AAG)-induced AXL receptor tyrosine kinase degradation.
  JOURNAL   J Biol Chem. 2013 Jun 14;288(24):17481-94. doi: 
            10.1074/jbc.M112.439422. Epub 2013 Apr 29.
  PUBMED    23629654
REFERENCE   2  (bases 1 to 7848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.439422"; journalName: "J Biol Chem"; date: 
            "2013-06-14- 14"; volume: "288"; issue: "24"; pages: "17481-94"
COMMENT     Sequence Label: pcDNA AXL
FEATURES             Location/Qualifiers
     source          1..7848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        170..549
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        550..753
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     704..724
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    755..773
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    776..794
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     804..828
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3706..3735
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3751..3768
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3791..3808)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3797..4021
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4067..4495
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4154..4173)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4364..4385
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4504..4524)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4509..4838
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4689..4824
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4751..4770
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4886..4933
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4952..5326
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5456..5577
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5493..5512)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5547..5566
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5626..5642)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5626..5642)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5639..5661)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5650..5666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5674..5680,5681..5698,5699..5704))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5719..5740
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5857..5874)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6028..6616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6108..6127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6787..7578,7579..7647))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7410..7429
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7648..7752)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7827..7846)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
       61 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
      121 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
      181 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      241 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      301 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      361 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      421 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      481 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      541 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      601 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      661 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      721 cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag agatctccct
      781 atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      841 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta
      901 gcgtttaaac ttaagcttgg taccgagctc ggatccagcc caacaacttc tgaggaaagt
      961 ttggcaccca tggcgtggcg gtgccccagg atgggcaggg tcccgctggc ctggtgcttg
     1021 gcgctgtgcg gctgggcgtg catggccccc aggggcacgc aggctgaaga aagtcccttc
     1081 gtgggcaacc cagggaatat cacaggtgcc cggggactca cgggcaccct tcggtgtcag
     1141 ctccaggttc agggagagcc ccccgaggta cattggcttc gggatggaca gatcctggag
     1201 ctcgcggaca gcacccagac ccaggtgccc ctgggtgagg atgaacagga tgactggata
     1261 gtggtcagcc agctcagaat cacctccctg cagctttccg acacgggaca gtaccagtgt
     1321 ttggtgtttc tgggacatca gaccttcgtg tcccagcctg gctatgttgg gctggagggc
     1381 ttgccttact tcctggagga gcccgaagac aggactgtgg ccgccaacac ccccttcaac
     1441 ctgagctgcc aagctcaggg acccccagag cccgtggacc tactctggct ccaggatgct
     1501 gtccccctgg ccacggctcc aggtcacggc ccccagcgca gcctgcatgt tccagggctg
     1561 aacaagacat cctctttctc ctgcgaagcc cataacgcca agggggtcac cacatcccgc
     1621 acagccacca tcacagtgct cccccagcag ccccgtaacc tccacctggt ctcccgccaa
     1681 cccacggagc tggaggtggc ttggactcca ggcctgagcg gcatctaccc cctgacccac
     1741 tgcaccctgc aggctgtgct gtcagacgat gggatgggca tccaggcggg agaaccagac
     1801 cccccagagg agcccctcac ctcgcaagca tccgtgcccc cccatcagct tcggctaggc
     1861 agcctccatc ctcacacccc ttatcacatc cgcgtggcat gcaccagcag ccagggcccc
     1921 tcatcctgga cccactggct tcctgtggag acgccggagg gagtgcccct gggcccccct
     1981 gagaacatta gtgctacgcg gaatgggagc caggccttcg tgcattggca agagccccgg
     2041 gcgcccctgc agggtaccct gttagggtac cggctggcgt atcaaggcca ggacacccca
     2101 gaggtgctaa tggacatagg gctaaggcaa gaggtgaccc tggagctgca gggggacggg
     2161 tctgtgtcca atctgacagt gtgtgtggca gcctacactg ctgctgggga tggaccctgg
     2221 agcctcccag tacccctgga ggcctggcgc ccagggcaag cacagccagt ccaccagctg
     2281 gtgaaggaac cttcaactcc tgccttctcg tggccctggt ggtatgtact gctaggagca
     2341 gtcgtggccg ctgcctgtgt cctcatcttg gctctcttcc ttgtccaccg gcgaaagaag
     2401 gagacccgtt atggagaagt gtttgaacca acagtggaaa gaggtgaact ggtagtcagg
     2461 taccgcgtgc gcaagtccta cagtcgtcgg accactgaag ctaccttgaa cagcttgggc
     2521 atcagtgaag agctgaagga gaagctgcgg gatgtgatgg tggaccggca caaggtggcc
     2581 ctggggaaga ctctgggaga gggagagttt ggagctgtga tggaaggcca gctcaaccag
     2641 gacgactcca tcctcaaggt ggctgtgaag acgatgaaga ttgccatctg cacgaggtca
     2701 gagctggagg atttcctgag tgaagcggtc tgcatgaagg aatttgacca tcccaacgtc
     2761 atgaggctca tcggtgtctg tttccagggt tctgaacgag agagcttccc agcacctgtg
     2821 gtcatcttac ctttcatgaa acatggagac ctacacagct tcctcctcta ttcccggctc
     2881 ggggaccagc cagtgtacct gcccactcag atgctagtga agttcatggc agacatcgcc
     2941 agtggcatgg agtatctgag taccaagaga ttcatacacc gggacctggc ggccaggaac
     3001 tgcatgctga atgagaacat gtccgtgtgt gtggcggact tcgggctctc caagaagatc
     3061 tacaatgggg actactaccg ccagggacgt atcgccaaga tgccagtcaa gtggattgcc
     3121 attgagagtc tagctgaccg tgtctacacc agcaagagcg atgtgtggtc cttcggggtg
     3181 acaatgtggg agattgccac aagaggccaa accccatatc cgggcgtgga gaacagcgag
     3241 atttatgact atctgcgccg gggaaatcgc ctgaagcagc ctgcggactg tctggatgga
     3301 ctgtatgcct tgatgtcgcg gtgctgggag ctaaatcccc aggaccggcc aagttttaca
     3361 gagctgcggg aagatttgga gaacacactg aaggccttgc ctcctgccca ggagcctgac
     3421 gaaatcctct atgtcaacat ggatgagggt ggaggttatc ctgaaccccc tggagctgca
     3481 ggaggagctg accccccaac ccagccagac cctaaggatt cctgtagctg cctcactgcg
     3541 gctgaggtcc atcctgctgg acgctatgtc ctctgccctt ccacaacccc tagccccgct
     3601 cagcctgctg ataggggctc cccagcagcc ccagggcagg aggatggtgc cgaattctgc
     3661 agatatccag cacagtggcg gccgctcgag tctagagggc ccttcgaaca aaaactcatc
     3721 tcagaagagg atctgaatat gcataccggt catcatcacc atcaccattg agtttaaacc
     3781 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     3841 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     3901 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     3961 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     4021 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     4081 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4141 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4201 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4261 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     4321 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4381 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     4441 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     4501 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     4561 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     4621 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     4681 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     4741 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     4801 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     4861 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     4921 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
     4981 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     5041 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     5101 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     5161 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     5221 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     5281 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
     5341 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     5401 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     5461 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5521 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5581 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     5641 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     5701 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     5761 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     5821 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     5881 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     5941 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6001 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6061 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     6481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     6781 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6841 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6901 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6961 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7021 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7081 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7141 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7201 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7261 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7321 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7381 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7441 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7501 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7561 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7621 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7681 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     7741 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     7801 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgca
//
