LOCUS       sequence-20437-e         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG303GAL-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(38..162)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(170..186)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(170..186)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(201..642)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(207..230)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    525..642
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(661..680)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(662..680)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(687..704)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(687..703)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taaagtgtaa agcctggggg tggcctaatg cggccgccat agtgactgga tatgttgtgt
       61 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
      121 atcattttac gtttctcgtt cagctttttt gtacaaactt gtgatggggg atccactagt
      181 tctagaatcc ggggtttttt ctccttgacg ttaaagtata gaggtatatt aacaattttt
      241 tgttgatact tttattacat ttgaataaga agtaatacaa accgaaaatg ttgaaagtat
      301 tagttaaagt ggttatgcag tttttgcatt tatatatctg ttaatagatc aaaaatcatc
      361 gcttcgctga ttaattaccc cagaaataag gctaaaaaac taatcgcatt atcatcctat
      421 ggttgttaat ttgattcgtt catttgaagg tttgtggggc caggttactg ccaatttttc
      481 ctcttcataa ccataaaagc tagtattgta gaatctttat tgttcggagc agtgcggcgc
      541 gaggcacatc tgcgtttcag gaacgcgacc ggtgaagacg aggacgcacg gaggagagtc
      601 ttccttcgga gggctgtcac ccgctcggcg gcttctaatc cgtactagag ctccaattcg
      661 ccctatagtg agtcgtatta caattcactg gccgtcgttt taca
//
