LOCUS       sequence-204453-        4552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish codon optimized B3 recombinase that acts on blown-out 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Tabor KM, Smith TS, Brown M, Bergeron SA, Briggman KL, Burgess HA
  TITLE     Presynaptic Inhibition Selectively Gates Auditory Transmission to 
            the Brainstem Startle Circuit.
  JOURNAL   Curr Biol. 2018 Aug 20;28(16):2527-2535.e8. doi: 
            10.1016/j.cub.2018.06.020. Epub 2018 Aug 2.
  PUBMED    30078569
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2018.06.020"; journalName: "Curr Biol"; date: 
            "2018-08-20- 20"; volume: "28"; issue: "16"; pages: "2527-2535.e8"
COMMENT     Sequence Label: B3rec.zf1
FEATURES             Location/Qualifiers
     source          1..4552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2331..2347)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2331..2347)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2344..2366)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2355..2371
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2379..2385,2386..2403,2404..2409))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2424..2445
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2562..2579)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2733..3321)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2813..2832)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3492..4283,4284..4352))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4115..4134
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4353..4457)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4525..4543
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tccaatccaa tatgagtagt tatatggatc tagtggacga cgagcctgct
      481 accctgtacc acaagtttgt ggagtgcctg aaggctggag agaacttctg tggagacaag
      541 ctgtctggaa tcatcactat ggccatcctg aaggccatca aggctctgac tgaggtgaag
      601 aagaccacct tcaacaagta caagaccacc atcaagcagg gactccagta tgatgtggga
      661 tctagcacca tctcttttgt gtaccacctg aaggactgtg atgagctgag cagaggactg
      721 tctgatgctt ttgagcctta caagttcaag atcaagagca acaaggaggc cacctctttc
      781 aagaccctgt tcagaggacc ttcttttgga tctcagaaga actggagaaa gaaggaggtg
      841 gacagagagg tggacaacct gttccacagc actgagactg atgagagcat cttcaagttc
      901 atcctgaaca ccctggacag cattgagacc cagaccaaca ctgacagaca gaagactgtg
      961 ctgaccttca tcctgctgat gaccttcttc aactgctgca ggaacaatga cctgatgaat
     1021 gtggacccta gcaccttcaa gattgtgaag aacaagtttg tgggatacct gctccaggct
     1081 gaggtgaagc agaccaagac cagaaagagc agaaacatct tcttcttccc aatcagagag
     1141 aacagatttg acctgttcct ggctctgcat gacttcttca gaacctgcca gccaacccca
     1201 aagagcagac tgtctgacca ggtgtctgag cagaagtggc agctgttcag agacagcatg
     1261 gtgattgact acaacagatt cttcagaaag ttccctgctt ctccaatctt tgccatcaag
     1321 catggaccaa agtctcacct gggaagacac ctgatgaact ctttcctgca caagaatgag
     1381 ctggactctt gggccaactc tctgggaaac tggtcttctt ctcagaacca gagagagtct
     1441 ggagccagac tgggatacac tcatggagga agagacctgc ctcagcctct gtttggattc
     1501 ctggctggat actgtgtgag aaatgaggag ggacacattg tgggactggg actggagaag
     1561 gacatcaatg acctgtttga tggaatcatg gaccctctga atgagaagga ggacactgaa
     1621 atctgtgagt cttatggaga gtgggccaag attgtgagca aggatgtgct gatcttcctg
     1681 aagagatacc acagcaagaa tgcttgcaga agataccaga acagcaccct gtatgccaga
     1741 accttcctga agactgagtc tgtgaccctg tctggaagca agggatctga ggagccttct
     1801 tctcctgtga gaatcccaat cctgagcatg ggaaaggctt ctccttctga gggaagaaag
     1861 ctgagagctt ctgagcatgc caatgatgac aatgagattg agaagattga ctctgactct
     1921 tctcagtctg aggagatccc aattgagatg tctgactctg aggatgagac cactgctagc
     1981 aacatctctg gaatctacct ggacatgagc aaggccaaca gcaatgtggt gtactctcct
     2041 ccttctcaga ctggaagagc tgctggagct ggaagaaaga gaggagtggg aggaagaaga
     2101 actgtggaga gcaagagaag aagagtgctg gctccaatca acagaggatc ccacggattc
     2161 cctcctgagg tggaggagca ggatgatgga accctgccaa tgtcttgtgc tcaggagtct
     2221 ggaatggaca gacaccctgc tgcttgtgct tctgccagaa tcaatgtgta aaaatcggat
     2281 cccgggcccg tcgactgcag aggcctgcat gcaagcttgg cgtaatcatg gtcatagctg
     2341 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     2401 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     2461 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     2521 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     2581 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2641 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2701 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2761 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2821 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2881 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2941 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     3001 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     3061 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     3121 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     3181 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     3241 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3301 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3361 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3421 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3481 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3541 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3601 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3661 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3721 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3781 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3841 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3901 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3961 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     4021 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     4081 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     4141 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     4201 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     4261 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     4321 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4381 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4441 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     4501 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
